breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 426,675 A→C S280R (AGC→CGC)  tig → trigger factor
JC 474,234 Δ4 bp coding (1069‑1072/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
JC JC 1,306,035 IS150 (+) +3 bp coding (203‑205/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,131,466 (A)9→8 intergenic (‑125/+175) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,558,224 G→A intergenic (‑49/+69) yfgK ← / ← yfgL GTP‑binding protein EngA/protein assembly complex, lipoprotein component
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
RA 3,928,593 A→T N126I (AAC→ATC)  rho → transcription termination factor Rho
RA 4,108,495 T→G E3A (GAA→GCA)  metJ ← transcriptional repressor protein MetJ
RA 4,138,988 C→T A213V (GCG→GTG)  oxyR → DNA‑binding transcriptional dual regulator
RA 4,164,097 C→T P1081L (CCG→CTG)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3895000 3901930 6931 13 [0] [0] 56 rbsD–[yieO] rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 1884735 =38 (0.700)122 (2.280) 74/148 0.0 74.7% coding (469/567 nt) yebN conserved inner membrane protein
?REL606 = 1889406 45 (0.840)coding (554/1374 nt) yebQ predicted transporter
* ? REL606 = 3652533NA (NA)54 (1.020) 43/146 0.2 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system