Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A131 F1 I1 R1
|
13 |
244.3 |
15300636 |
95.6% |
14627408 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,595 |
G→A |
A127T (GCC→ACC) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,595 | 0 | G | A | 98.9%
| 1041.2
/ NA
| 277 | A127T (GCC→ACC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (137/136); minor base C (0/3); total (137/140) |
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 279 total aligned reads displayed.
CCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTA > REL606/3928522‑3928667
|
cccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACAcc > 1:4313616/1‑76 (MQ=255)
cgccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCg < 1:4581128/76‑1 (MQ=255)
gccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGc < 2:4308352/76‑1 (MQ=255)
aaaGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaaca > 2:4623266/1‑76 (MQ=255)
aagaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaacaa > 2:5444508/1‑76 (MQ=255)
aagaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaacaa < 1:4897166/76‑1 (MQ=255)
aagaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaacaa < 1:4069232/76‑1 (MQ=255)
agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaa > 1:4198169/1‑76 (MQ=255)
agaagGAGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaa > 2:5182765/1‑76 (MQ=255)
gaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaaa > 2:5305162/1‑76 (MQ=255)
gaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaaa < 1:3039217/76‑1 (MQ=255)
aagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAAt < 1:1510587/76‑1 (MQ=255)
gTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCct < 2:2910637/76‑1 (MQ=255)
gTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCct < 2:3893499/76‑1 (MQ=255)
gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCctct < 2:79198/76‑1 (MQ=255)
gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCctct > 2:6579642/1‑76 (MQ=255)
gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCctct > 1:747138/1‑76 (MQ=255)
cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 1:1185060/76‑1 (MQ=255)
cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 2:1538853/76‑1 (MQ=255)
cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 2:1261368/76‑1 (MQ=255)
cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 2:892259/76‑1 (MQ=255)
gCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTga < 1:982801/76‑1 (MQ=255)
cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgag < 1:5617501/76‑1 (MQ=255)
cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgag < 1:1577293/76‑1 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAGAAACCTGAAAACACCCGCAACAAAATCCTCTTTgaga < 1:1053599/76‑1 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgaga > 2:3342160/1‑76 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgaga > 1:5681478/1‑76 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTAAACTTCGACAAACCAGAAAACACCCGCAACAAAATCCTCTTTgaga < 2:3172193/76‑1 (MQ=255)
aTTTTNCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGaa > 2:2840412/1‑76 (MQ=255)
aTTTTGCGCTGCTGAATGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGaa < 1:3551903/76‑1 (MQ=255)
aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGaa < 1:6888798/76‑1 (MQ=255)
ttGCGCTGCTGAAAGTTAACGAAGTTAGCTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt < 2:931068/76‑1 (MQ=255)
ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt < 1:5837850/76‑1 (MQ=255)
ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt > 2:661349/1‑76 (MQ=255)
ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt > 1:3936767/1‑76 (MQ=255)
tGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACCCCCGCAACAAAATCCTCTTTGAGAACTTa < 2:2487634/76‑1 (MQ=255)
tGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTa > 2:7117376/1‑76 (MQ=255)
gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTaa < 1:596738/76‑1 (MQ=255)
cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 1:173415/76‑1 (MQ=255)
cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 1:6532902/76‑1 (MQ=255)
cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 2:395034/76‑1 (MQ=255)
cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 2:1847602/76‑1 (MQ=255)
gctgctGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAcc < 2:4871640/76‑1 (MQ=255)
gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 2:735735/1‑76 (MQ=255)
gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg < 2:1773407/76‑1 (MQ=255)
ctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGc > 2:1607599/1‑76 (MQ=255)
gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:3245516/1‑76 (MQ=255)
aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa > 2:5014876/1‑76 (MQ=255)
aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa < 2:3448573/76‑1 (MQ=255)
aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa > 1:2049814/1‑76 (MQ=255)
aGTTAACGTAGTCAACTTCGACAAACCTGAAAGCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc < 2:4501309/76‑1 (MQ=255)
aGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc < 1:5094990/76‑1 (MQ=255)
tAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 1:800513/1‑76 (MQ=255)
tAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 2:1934102/1‑76 (MQ=255)
aaCGAATTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa < 1:4838071/76‑1 (MQ=255)
aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 1:2558934/1‑76 (MQ=255)
aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 2:5790914/1‑76 (MQ=255)
aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 1:3744725/1‑76 (MQ=255)
aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa < 1:5208427/76‑1 (MQ=255)
aCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa < 2:5413181/76‑1 (MQ=255)
aCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa < 2:1636281/76‑1 (MQ=255)
cGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc < 1:4438408/76‑1 (MQ=255)
gAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct > 1:2827858/1‑76 (MQ=255)
gAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct > 2:4011710/1‑76 (MQ=255)
aGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct > 1:7042649/1‑76 (MQ=255)
gTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc < 2:7042749/76‑1 (MQ=255)
ttAACTTCTACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 1:1867167/1‑76 (MQ=255)
ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 1:6443982/1‑76 (MQ=255)
ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 2:418220/1‑76 (MQ=255)
ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 2:5601625/1‑76 (MQ=255)
ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 1:3405925/1‑76 (MQ=255)
tAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt < 2:5695774/76‑1 (MQ=255)
tAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt > 2:6317006/1‑76 (MQ=255)
aCTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:6029308/76‑1 (MQ=255)
aCTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:2167525/76‑1 (MQ=255)
aCTTCCACAAACCTGAAACCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:6322194/76‑1 (MQ=255)
cTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg < 1:3180226/76‑1 (MQ=255)
ttCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc > 1:5428027/1‑76 (MQ=255)
cGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt > 1:720721/1‑76 (MQ=255)
cGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt < 2:7467669/76‑1 (MQ=255)
gACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa < 2:2752768/76‑1 (MQ=255)
gACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa > 2:2775845/1‑76 (MQ=255)
aCAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTAt > 1:4996996/1‑76 (MQ=255)
cAAACCTGAAAACACCCGCAACAAAATCTTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg > 2:1368624/1‑76 (MQ=255)
aaaCCTGAAAACCCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg < 1:927177/76‑1 (MQ=255)
aaaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg > 2:3736734/1‑76 (MQ=255)
aaaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg < 2:2370122/76‑1 (MQ=255)
aaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa < 2:6700511/76‑1 (MQ=255)
aaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa < 2:2206721/76‑1 (MQ=255)
aCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa > 1:4379071/1‑76 (MQ=255)
ccTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc > 1:266196/1‑76 (MQ=255)
cTGAAAACCCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg < 1:626247/76‑1 (MQ=255)
cTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg < 2:6184487/76‑1 (MQ=255)
cTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg > 2:2684706/1‑76 (MQ=255)
taaaaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt < 1:5858939/74‑1 (MQ=255)
gAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAATCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg < 2:567408/76‑1 (MQ=255)
aaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt > 1:1418055/1‑76 (MQ=255)
aaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt < 2:3851866/76‑1 (MQ=255)
aaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt < 1:668775/76‑1 (MQ=255)
aaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTa > 1:4712480/1‑76 (MQ=255)
|
CCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTA > REL606/3928522‑3928667
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
