Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A45 F1 I1 R1
|
14 |
53.1 |
3307936 |
97.7% |
3231853 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,601 |
A→G |
N129D (AAC→GAC) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,601 | 0 | A | G | 98.1%
| 214.7
/ ‑4.2
| 52 | N129D (AAC→GAC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (32/19); total (33/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTT > REL606/3928528‑3928673
|
gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcgac < 2:1184438/76‑1 (MQ=255)
gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcgac > 2:1534188/1‑76 (MQ=255)
aaaGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcgaca > 2:1384093/1‑76 (MQ=255)
agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACaaa < 1:40574/76‑1 (MQ=255)
agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACaaa > 1:1644625/1‑76 (MQ=255)
tGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctc > 1:1031147/1‑76 (MQ=255)
tGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctc > 2:1523730/1‑76 (MQ=255)
tGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctc < 2:898664/76‑1 (MQ=255)
gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctct > 1:389599/1‑76 (MQ=255)
gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctct > 1:1300499/1‑76 (MQ=255)
aaCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCtt > 2:1447820/1‑76 (MQ=255)
aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCttt < 2:687784/76‑1 (MQ=255)
cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgag < 1:296370/76‑1 (MQ=255)
ttttttGCGCTGCTAAAATTTAACAAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgaga < 1:1146271/74‑1 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgaga > 1:491892/1‑76 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgaga < 2:1238234/76‑1 (MQ=255)
aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGaa > 1:295316/1‑76 (MQ=255)
aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGaa < 2:120685/76‑1 (MQ=255)
tttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACt > 2:1376432/1‑76 (MQ=255)
tttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACt > 1:674868/1‑76 (MQ=255)
tGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTa < 2:289132/76‑1 (MQ=255)
gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTaa > 1:1442141/1‑76 (MQ=255)
gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTaa < 2:1143266/76‑1 (MQ=255)
gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:747810/1‑76 (MQ=255)
gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:1337386/1‑76 (MQ=255)
gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:506539/1‑76 (MQ=255)
gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:282126/1‑76 (MQ=255)
aaaGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGc > 1:799095/1‑76 (MQ=255)
aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa < 1:695500/76‑1 (MQ=255)
aGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc > 2:1034224/1‑76 (MQ=255)
aGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc < 1:49945/76‑1 (MQ=255)
gTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACg > 2:1403376/1‑76 (MQ=255)
ttAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGc > 1:39186/1‑76 (MQ=255)
aaCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 1:834827/1‑76 (MQ=255)
aaCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa < 2:790667/76‑1 (MQ=255)
cGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc > 2:1559948/1‑76 (MQ=255)
gAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct > 2:943710/1‑76 (MQ=255)
ttAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg < 2:738588/76‑1 (MQ=255)
aaCTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc > 2:252801/1‑76 (MQ=255)
aaCTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc > 1:1451827/1‑76 (MQ=255)
ttCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc < 1:1534188/76‑1 (MQ=255)
ttCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc > 2:841601/1‑76 (MQ=255)
cGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt > 1:181702/1‑76 (MQ=255)
cAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg > 1:1132908/1‑76 (MQ=255)
cAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg < 1:710923/76‑1 (MQ=255)
aaCCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa > 2:1056844/1‑76 (MQ=255)
aCCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa < 1:1642904/76‑1 (MQ=255)
cTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg < 2:1515860/76‑1 (MQ=255)
cTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg > 1:447167/1‑76 (MQ=255)
tGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt > 2:1115321/1‑76 (MQ=255)
gAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 2:934833/1‑76 (MQ=255)
gAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 1:708528/1‑76 (MQ=255)
aaaCGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt < 1:1565454/76‑1 (MQ=255)
aCGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa > 1:923053/1‑76 (MQ=255)
aCGCCCGCGACAAAAACCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa < 2:324544/76‑1 (MQ=255)
cgcgACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt < 2:458501/76‑1 (MQ=255)
cgcgACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt > 2:1088206/1‑76 (MQ=255)
|
GAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTT > REL606/3928528‑3928673
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
