Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A133 F1 I1 R1
|
21 |
238.6 |
15028552 |
95.5% |
14352267 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,164,917 |
A→C |
S309A (TCG→GCG) |
yehU ← |
predicted sensory kinase in two‑component system with YehT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,164,917 | 0 | A | C | 99.5%
| 817.9
/ ‑3.9
| 189 | S309A (TCG→GCG) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (84/104); total (84/105) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 |
Only 100 of 194 total aligned reads displayed.
TTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCACC > REL606/2164844‑2164989
|
ttttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGcccc > 1:2601219/1‑76 (MQ=255)
tttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCcccc > 1:402690/1‑76 (MQ=255)
tttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCcccc < 2:6215995/76‑1 (MQ=255)
ttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCa > 2:495166/1‑76 (MQ=255)
ttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGCCCCCa < 2:3982028/76‑1 (MQ=255)
tGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAg > 2:6585289/1‑76 (MQ=255)
gCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGtt > 1:4202893/1‑76 (MQ=255)
cTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGttt < 2:7164360/76‑1 (MQ=255)
tCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGc > 2:591422/1‑76 (MQ=255)
tCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGc < 1:7400928/76‑1 (MQ=255)
cATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCa < 2:48640/76‑1 (MQ=255)
aTACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAgcatgcat > 2:1808743/1‑76 (MQ=255)
tACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCAtt < 1:5768075/76‑1 (MQ=255)
tACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCAtt < 2:6347841/76‑1 (MQ=255)
aaTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCg > 1:1001943/1‑76 (MQ=255)
aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCgg < 1:2104612/76‑1 (MQ=255)
aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCgg > 1:1166282/1‑76 (MQ=255)
aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCgg > 2:250096/1‑76 (MQ=255)
tttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGa > 1:4340284/1‑76 (MQ=255)
tttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGa > 1:1423228/1‑76 (MQ=255)
ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt < 2:6856713/76‑1 (MQ=255)
ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt > 2:3214404/1‑76 (MQ=255)
ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt < 1:2882266/76‑1 (MQ=255)
ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt < 1:1651051/76‑1 (MQ=255)
gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc < 2:5268759/76‑1 (MQ=255)
gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc > 2:1023153/1‑76 (MQ=255)
gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc > 1:5589595/1‑76 (MQ=255)
gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc > 1:3596060/1‑76 (MQ=255)
aTGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCa < 1:6057156/76‑1 (MQ=255)
ggTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCaaa < 1:5306661/76‑1 (MQ=255)
gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag < 1:2311298/76‑1 (MQ=255)
gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag > 1:3290609/1‑76 (MQ=255)
gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag < 1:235936/76‑1 (MQ=255)
gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag < 2:1430663/76‑1 (MQ=255)
tGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAaga < 1:1937931/76‑1 (MQ=255)
tGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAaga > 2:3689152/1‑76 (MQ=255)
gCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAagag > 1:6415949/1‑76 (MQ=255)
gCCCATCACGCACTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAagag > 1:856132/1‑76 (MQ=255)
cccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGc < 2:3576713/76‑1 (MQ=255)
ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCTCCCCCAGTTTGCATTGCGGATGCAAAGAGCa < 2:4612952/76‑1 (MQ=255)
ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCa < 2:2298505/76‑1 (MQ=255)
ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCa < 2:6041762/76‑1 (MQ=255)
ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCa > 2:2763742/1‑76 (MQ=255)
cATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCaa > 2:6947918/1‑76 (MQ=255)
cATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCaa > 2:1288603/1‑76 (MQ=255)
cATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCaa < 2:5771617/76‑1 (MQ=255)
tCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACg < 1:1796524/76‑1 (MQ=255)
tCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACg < 1:5405264/76‑1 (MQ=255)
aCGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGAt < 1:203585/76‑1 (MQ=255)
cgcgCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATa > 1:6584167/1‑76 (MQ=255)
gcgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATaa < 1:4569165/76‑1 (MQ=255)
gcgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATaa > 1:2931618/1‑76 (MQ=255)
cgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAg > 1:705800/1‑76 (MQ=255)
cgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAg < 2:1052847/76‑1 (MQ=255)
gcTGATTTTCGCCACTCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg < 2:2846534/76‑1 (MQ=255)
gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 2:2001703/1‑76 (MQ=255)
gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 1:2138870/1‑76 (MQ=255)
gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 1:2666542/1‑76 (MQ=255)
gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 2:758490/1‑76 (MQ=255)
cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 2:345168/76‑1 (MQ=255)
cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 1:2443013/76‑1 (MQ=255)
cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 2:5077101/76‑1 (MQ=255)
cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt > 1:5265935/1‑76 (MQ=255)
cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 2:4577710/76‑1 (MQ=255)
tGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTa < 1:6600532/76‑1 (MQ=255)
aTTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACt < 1:409410/76‑1 (MQ=255)
ttttCGCGACGCAACGAAATTACCAGCCTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACtt < 1:7365718/76‑1 (MQ=255)
ttttCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACtt > 2:1599819/1‑76 (MQ=255)
ttttCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACtt < 1:5875962/76‑1 (MQ=255)
tttCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTc > 2:7440215/1‑76 (MQ=255)
cGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGtt < 1:3393222/76‑1 (MQ=255)
cGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGtt < 1:5776347/76‑1 (MQ=255)
gCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTg < 1:2758685/76‑1 (MQ=255)
ccACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGc > 1:3929251/1‑76 (MQ=255)
cGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCAt > 1:1004180/1‑76 (MQ=255)
gCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATc < 2:3902450/76‑1 (MQ=255)
cAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCa < 1:2849960/76‑1 (MQ=255)
cAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCa < 1:315885/76‑1 (MQ=255)
cAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCa > 1:5194834/1‑76 (MQ=255)
aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg < 1:6935240/76‑1 (MQ=255)
aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg > 1:2590938/1‑76 (MQ=255)
aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg > 2:6087161/1‑76 (MQ=255)
aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg > 2:120111/1‑76 (MQ=255)
aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg < 2:2629693/76‑1 (MQ=255)
aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg < 2:3643225/76‑1 (MQ=255)
aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 2:2653786/1‑76 (MQ=255)
aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 1:1490515/1‑76 (MQ=255)
aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 2:1925880/1‑76 (MQ=255)
aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 2:4160400/1‑76 (MQ=255)
tcccAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc < 1:6781603/74‑1 (MQ=255)
tACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc > 2:2033476/1‑76 (MQ=255)
tACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc > 1:6117618/1‑76 (MQ=255)
aCCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCa > 1:4851873/1‑76 (MQ=255)
ccAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCAc < 2:4892187/76‑1 (MQ=255)
cAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACt < 1:5445608/76‑1 (MQ=255)
cAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACt < 1:929203/76‑1 (MQ=255)
cAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACt > 1:7376786/1‑76 (MQ=255)
gCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTc < 2:2687783/76‑1 (MQ=255)
cGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCa < 1:1730642/76‑1 (MQ=255)
gTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCAc < 2:4183053/76‑1 (MQ=255)
|
TTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCACC > REL606/2164844‑2164989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
