Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A133 F1 I1 R1
|
21 |
238.6 |
15028552 |
95.5% |
14352267 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,762,213 |
T→G |
Y67S (TAT→TCT) |
rpoS ← |
RNA polymerase sigma factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,762,213 | 0 | T | G | 99.2%
| 1019.2
/ ‑3.4
| 238 | Y67S (TAT→TCT) | rpoS | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (136/100); total (138/100) |
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 247 total aligned reads displayed.
GCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCT > REL606/2762140‑2762285
|
gcgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTgaagaa < 1:3526046/76‑1 (MQ=255)
cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAAc > 2:6027470/1‑76 (MQ=255)
cgAGAGGCGACATCTCCACGCAGGGCGTGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGCGGTGAAGAAc > 1:7152923/1‑76 (MQ=255)
gagaGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAAcc < 2:2782214/76‑1 (MQ=255)
gagaGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAAcc < 1:6216609/76‑1 (MQ=255)
agagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCa > 2:5755905/1‑76 (MQ=255)
gagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCaa > 1:5664142/1‑76 (MQ=255)
gagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCaa > 1:2558758/1‑76 (MQ=255)
agGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAAt > 1:5225506/1‑76 (MQ=255)
agGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAt < 2:4848616/76‑1 (MQ=255)
gCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtct < 2:4379516/76‑1 (MQ=255)
gCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtct < 1:3868156/76‑1 (MQ=255)
cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtctc < 1:2226240/76‑1 (MQ=255)
cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtctc > 1:3371453/1‑76 (MQ=255)
cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtctc > 1:2313019/1‑76 (MQ=255)
gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCa < 1:3578036/76‑1 (MQ=255)
gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCa < 1:5007641/76‑1 (MQ=255)
gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCa < 1:2287135/76‑1 (MQ=255)
cATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCAca > 2:5448822/1‑75 (MQ=255)
aTCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCa < 1:6636731/76‑1 (MQ=255)
tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAgg < 2:2926062/76‑1 (MQ=255)
tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAgg > 2:7393813/1‑76 (MQ=255)
ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGt > 2:6938358/1‑76 (MQ=255)
cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTa < 2:2351880/76‑1 (MQ=255)
aCGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaa < 2:1131120/76‑1 (MQ=255)
cGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaaa < 1:6724648/76‑1 (MQ=255)
cGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaaa > 1:5376181/1‑76 (MQ=255)
gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTCAAg > 2:7269783/1‑76 (MQ=255)
gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAg > 1:4910967/1‑76 (MQ=255)
gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAg > 2:822081/1‑76 (MQ=255)
cAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGc > 2:3315463/1‑76 (MQ=255)
cAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGc > 2:1190058/1‑76 (MQ=255)
cAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGCAAAGc > 2:7372310/1‑76 (MQ=255)
aGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCt < 1:3135283/76‑1 (MQ=255)
gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGTCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 2:3037763/76‑1 (MQ=255)
gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 1:1725711/76‑1 (MQ=255)
gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 2:5780837/76‑1 (MQ=255)
gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 2:3433203/76‑1 (MQ=255)
cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGt > 2:5141553/1‑76 (MQ=255)
cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGt < 1:2798545/76‑1 (MQ=255)
gcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTc < 1:4173905/76‑1 (MQ=255)
aCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTcgcg < 1:1410243/76‑1 (MQ=255)
aCGCGCAAAATAAACTTCTTCTCCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTcgcg < 1:4557648/76‑1 (MQ=255)
gcgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc > 1:1094695/1‑76 (MQ=255)
gcgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc > 1:6806813/1‑76 (MQ=255)
gcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCa > 2:5800030/1‑76 (MQ=255)
aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 2:366074/1‑76 (MQ=255)
aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 2:7078107/1‑76 (MQ=255)
aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 1:2887162/1‑76 (MQ=255)
aaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAaca > 2:3852154/1‑76 (MQ=255)
aaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAaca < 1:3919470/76‑1 (MQ=255)
aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac > 2:3291613/1‑76 (MQ=255)
aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac < 1:5145496/76‑1 (MQ=255)
aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac < 1:7045278/76‑1 (MQ=255)
aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg < 2:4169055/76‑1 (MQ=255)
aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg > 2:3153051/1‑76 (MQ=255)
aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg > 2:6820030/1‑76 (MQ=255)
aaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGc < 2:1653097/76‑1 (MQ=255)
aCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCt > 2:6174019/1‑76 (MQ=255)
cttcttcttCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtg > 1:4503186/1‑76 (MQ=255)
tcttcttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtg < 2:1440025/76‑1 (MQ=255)
cttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGc > 2:4318006/1‑76 (MQ=255)
cttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGc > 1:3272872/1‑76 (MQ=255)
cttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGc > 1:5813595/1‑76 (MQ=255)
ttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt > 2:3552839/1‑76 (MQ=255)
ttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt < 2:6358138/76‑1 (MQ=255)
tcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTc > 1:1973345/1‑76 (MQ=255)
tcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTc > 1:1166802/1‑76 (MQ=255)
cGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTcc > 1:4317404/1‑76 (MQ=255)
gCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCt > 2:2837899/1‑76 (MQ=255)
gCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCt < 2:5900728/76‑1 (MQ=255)
ccGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTg < 2:4535836/76‑1 (MQ=255)
cGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGc < 1:4759415/76‑1 (MQ=255)
cGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGc > 2:6959972/1‑76 (MQ=255)
gTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCttgg > 2:3738862/1‑73 (MQ=255)
gTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCg > 1:3198749/1‑76 (MQ=255)
gTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCg < 1:1015854/76‑1 (MQ=255)
ttAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:1785966/1‑76 (MQ=255)
ttAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:5550111/1‑76 (MQ=255)
ttAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:2133143/1‑76 (MQ=255)
tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 2:2230044/1‑76 (MQ=255)
tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 2:2622288/1‑76 (MQ=255)
tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 2:1281423/1‑76 (MQ=255)
tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGACCAACACACGCTGTGTGGCTCCCTGCGAt < 2:7505290/76‑1 (MQ=255)
aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:1716306/1‑76 (MQ=255)
aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:6723273/1‑76 (MQ=255)
aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:4791299/1‑76 (MQ=255)
aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa < 1:5949366/76‑1 (MQ=255)
aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa < 1:2635876/76‑1 (MQ=255)
aCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATaa < 2:2522535/76‑1 (MQ=255)
cAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAAc < 1:6819739/76‑1 (MQ=255)
cAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAAc < 1:2961920/76‑1 (MQ=255)
aGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa < 1:1031929/76‑1 (MQ=255)
aGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa < 1:193221/76‑1 (MQ=255)
aGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa < 2:3215832/76‑1 (MQ=255)
gtggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAg < 1:871294/76‑1 (MQ=255)
tggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGt > 2:1997548/1‑76 (MQ=255)
tggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGt < 1:938621/76‑1 (MQ=255)
ggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGtt < 2:246654/76‑1 (MQ=255)
gaagaaCCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCct < 2:3919538/76‑1 (MQ=255)
|
GCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCT > REL606/2762140‑2762285
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
