Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A133 F1 I1 R1
|
21 |
238.6 |
15028552 |
95.5% |
14352267 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,027,164 |
(A)6→5 |
coding (507/660 nt) |
ECB_02827 ← |
Polysialic acid transport ATP‑binding KpsT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,027,159 | 0 | A | . | 95.9%
| 694.2
/ 21.5
| 146 | coding (512/660 nt) | ECB_02827 | Polysialic acid transport ATP‑binding KpsT |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base . (81/59); total (83/63) |
| Fisher's exact test for biased strand distribution p-value = 4.03e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
Only 100 of 168 total aligned reads displayed.
ACCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACTG > REL606/3027084‑3027231
|
aCCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTa < 2:3081674/76‑1 (MQ=255)
aCCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTa < 1:852213/76‑1 (MQ=255)
ccGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaa < 1:2103063/76‑1 (MQ=255)
cgTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaa < 1:5256383/76‑1 (MQ=255)
ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact > 1:501685/1‑74 (MQ=255)
ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact < 1:5871711/76‑3 (MQ=255)
ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact > 1:6972551/1‑74 (MQ=255)
ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact > 2:225087/1‑74 (MQ=255)
tATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaacta > 2:1835078/1‑73 (MQ=255)
tATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATCCAAACTATGTGAAACCATTaaaaacta > 2:5219596/1‑73 (MQ=255)
aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt > 1:1752199/1‑76 (MQ=255)
aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt < 1:661210/76‑1 (MQ=255)
aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt < 1:3529445/76‑1 (MQ=255)
aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt < 1:5943175/76‑1 (MQ=255)
ttttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCt < 1:3038305/76‑1 (MQ=255)
tttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCtt < 2:7233639/76‑1 (MQ=255)
ttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCttt > 1:6335743/1‑76 (MQ=255)
ttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCttt > 1:6042181/1‑76 (MQ=255)
ttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCttt > 1:4912005/1‑76 (MQ=255)
tAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTa > 1:6832602/1‑76 (MQ=255)
aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt < 2:7089334/76‑1 (MQ=255)
aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt > 2:5435000/1‑76 (MQ=255)
aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt < 1:1134192/76‑1 (MQ=255)
aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt > 1:1402641/1‑76 (MQ=255)
aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt > 1:2179664/1‑76 (MQ=255)
aaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATg > 2:4241686/1‑76 (MQ=255)
aaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATg > 2:4397120/1‑76 (MQ=255)
aTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGc > 2:2700937/1‑76 (MQ=255)
aTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGc > 2:6447183/1‑76 (MQ=255)
aTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGc > 1:7506529/1‑76 (MQ=255)
aCAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCct < 1:6922577/76‑1 (MQ=255)
aCAAAGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCct > 1:5079954/1‑76 (MQ=255)
cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt < 2:6987992/76‑1 (MQ=255)
cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt > 1:1586668/1‑76 (MQ=255)
cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt > 1:3475078/1‑76 (MQ=255)
cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt > 1:329117/1‑76 (MQ=255)
ggCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttctt > 1:2266732/1‑76 (MQ=255)
ggCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttctt > 1:4483795/1‑76 (MQ=255)
gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 1:3116442/76‑1 (MQ=255)
gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 2:744120/76‑1 (MQ=255)
gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 2:4849979/76‑1 (MQ=255)
gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 2:2333286/76‑1 (MQ=255)
cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa > 2:5913769/1‑76 (MQ=255)
cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa < 1:2832092/76‑1 (MQ=255)
cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa > 2:1241057/1‑76 (MQ=255)
cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa < 2:4793364/76‑1 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa > 1:6694491/1‑76 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:3213671/76‑1 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:2665324/76‑1 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:4406166/76‑1 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 1:5932742/76‑1 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:4529008/76‑1 (MQ=255)
acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa > 1:7395617/1‑76 (MQ=255)
caTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAAc < 2:5083541/76‑1 (MQ=255)
caTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAAc > 2:6627761/1‑76 (MQ=255)
aTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACa > 2:2090003/1‑76 (MQ=255)
aTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACa < 2:5111184/76‑1 (MQ=255)
cGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAt > 2:5350775/1‑76 (MQ=255)
cGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAt > 1:3225419/1‑76 (MQ=255)
gCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAtt < 1:2062286/76‑1 (MQ=255)
gCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAtt > 1:97422/1‑76 (MQ=255)
cAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTg < 2:50214/76‑1 (MQ=255)
aaaCTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAg < 1:4020977/76‑1 (MQ=255)
aaaCTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAg > 2:1293403/1‑76 (MQ=255)
aCTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAgcg > 1:5013188/1‑76 (MQ=255)
tctTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCa > 2:6594402/1‑76 (MQ=255)
tctTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCa > 1:95118/1‑76 (MQ=255)
tttCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAtt > 2:3527318/1‑76 (MQ=255)
tCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAtttt < 2:6257056/76‑1 (MQ=255)
tCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAtttt < 1:6033117/76‑1 (MQ=255)
cAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAttttt < 2:4640446/76‑1 (MQ=255)
aaTGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTc > 2:6237492/1‑76 (MQ=255)
aaTGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTc > 2:1659653/1‑76 (MQ=255)
gAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCttt > 2:855305/1‑76 (MQ=255)
aaTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTa < 1:630819/76‑1 (MQ=255)
aaTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTa < 2:1501532/76‑1 (MQ=255)
aTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTaa > 1:4151539/1‑76 (MQ=255)
aTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTaa > 1:5743788/1‑76 (MQ=255)
ttCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTaaa > 1:5524204/1‑76 (MQ=255)
tCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAc > 2:3041722/1‑76 (MQ=255)
cAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAcc > 1:67905/1‑76 (MQ=255)
cAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAcc < 2:5789676/76‑1 (MQ=255)
cAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAcc > 2:5298015/1‑76 (MQ=255)
aaaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCt > 2:3857681/1‑76 (MQ=255)
aaaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCt > 2:2554798/1‑76 (MQ=255)
aaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTg > 1:6203290/1‑76 (MQ=255)
aaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTg < 2:2847412/76‑1 (MQ=255)
tATGTGAAACCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGc > 2:5478355/1‑76 (MQ=255)
tATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCa > 2:2790273/1‑76 (MQ=255)
tATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCa > 1:6450079/1‑76 (MQ=255)
tATGTGAAACCATT‑AAAAACTAGATTCTTTAGGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCa > 2:5805140/1‑76 (MQ=255)
aTGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCAt < 1:1250876/76‑1 (MQ=255)
gtgAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCa > 2:4017279/1‑76 (MQ=255)
gAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCAcc > 2:5508415/1‑76 (MQ=255)
aaaCCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCAcc < 2:1716487/76‑1 (MQ=255)
aaCCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGa < 2:1499975/76‑1 (MQ=255)
aCCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGAc < 1:2843386/76‑1 (MQ=255)
ccATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACt < 2:5566628/76‑1 (MQ=255)
ccATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACt < 1:5347097/76‑1 (MQ=255)
aTTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACTg > 2:3437921/1‑76 (MQ=255)
|
ACCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACTG > REL606/3027084‑3027231
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
