Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
18 |
38.4 |
2430560 |
95.7% |
2326045 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,096,926 |
A→G |
L216P (CTG→CCG) |
glpF ← |
glycerol facilitator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,096,926 | 0 | A | G | 97.1%
| 125.9
/ ‑3.4
| 34 | L216P (CTG→CCG) | glpF | glycerol facilitator |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (18/15); total (18/16) |
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01 |
GCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGAT > REL606/4096856‑4096997
|
gcaaaaaaGCGCCGCGAGGAAGTAAGGAATGTCTCGGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGccggccag < 1:364038/73‑1 (MQ=255)
caaaaaaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAgg < 2:814171/74‑1 (MQ=255)
caaaaaaGCGGCACCAGGAAGTAAGGAATGTCTCGGCCGCCGGTAAAGGCGACATTGCCCCAGCACGCCGGCCAgg < 1:344274/74‑1 (MQ=255)
cAAAAAGCGGCACCAGAAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCGAGCCCGCCAGCCAGGc < 2:1011493/76‑1 (MQ=255)
aaaaaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCa > 2:463591/1‑76 (MQ=255)
aggggCACCAGGAATAAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGa < 1:168592/73‑1 (MQ=255)
aGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGGCTTTCGGAc < 2:549987/76‑1 (MQ=255)
tAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGt > 2:217734/1‑76 (MQ=255)
aaTGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCAcgc < 1:252908/76‑1 (MQ=255)
gTCTCTGNCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCt > 2:980490/1‑76 (MQ=255)
tcncCGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTTACGCCNTg < 2:993565/76‑1 (MQ=255)
ntctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTgg > 1:352197/2‑76 (MQ=255)
ctctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTgg > 2:303889/1‑76 (MQ=255)
ctctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTgg < 2:1061241/76‑1 (MQ=255)
ctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGt > 2:498483/1‑76 (MQ=255)
ctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGt > 2:340335/1‑76 (MQ=255)
ctGCCGCCGCTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGt > 2:212433/1‑76 (MQ=255)
gccgccGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTc > 2:121964/1‑76 (MQ=255)
gccgccGGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTc > 2:431510/1‑76 (MQ=255)
gccgGTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATg > 2:870614/1‑76 (MQ=255)
ccgGTAAAGGCGACATTGCCCCAGCNCTCCGGCCAGGCAAAGACTTANGGAGCGAAGTCACGCGCTGGGTTCATgg < 1:175082/76‑1 (MQ=255)
cgGTAAAGGCGACATTGCCCCAGCCCGCCGGCCGGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGc > 2:666982/1‑76 (MQ=255)
ggTAAAGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCa > 1:53335/1‑76 (MQ=255)
aaaGGCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCaaaa > 2:442567/1‑76 (MQ=255)
gCGACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCg < 2:493108/76‑1 (MQ=255)
gACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTc > 1:559629/1‑76 (MQ=255)
gACATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTc > 2:747617/1‑76 (MQ=255)
aCATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCa < 1:748347/76‑1 (MQ=255)
aCATTGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCa < 1:870614/76‑1 (MQ=255)
ttGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATg > 1:485101/1‑76 (MQ=255)
tGCCCCAGCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATgg > 2:403854/1‑76 (MQ=255)
cccACCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGcc < 2:630718/76‑1 (MQ=255)
gCCCGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATa < 2:625983/76‑1 (MQ=255)
cccGCCGGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAg < 1:618865/76‑1 (MQ=255)
ccgccgGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAACGGGCCCATAGa > 1:332568/1‑76 (MQ=255)
cGCCGGCCAGGCAAAGACTTTCGGACCGAGGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGAt < 2:390770/76‑1 (MQ=255)
|
GCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGAT > REL606/4096856‑4096997
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
