Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
14 |
24.7 |
1576304 |
96.5% |
1521133 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,489,130 |
A→G |
V26A (GTG→GCG) |
glpR ← |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,489,130 | 0 | A | G | 100.0%
| 109.3
/ NA
| 31 | V26A (GTG→GCG) | glpR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/17); total (14/17) |
CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTG > REL606/3489055‑3489200
|
gtgaGGATCAGGGGTTGCTCCGACAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTcca < 1:568588/73‑1 (MQ=255)
aGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTccgcca > 2:770502/1‑76 (MQ=255)
gATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGc > 1:60056/1‑76 (MQ=255)
aTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGGGCTCCGCCCGct > 1:357561/1‑76 (MQ=255)
cAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGcgcc > 2:283532/1‑76 (MQ=255)
aGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGctccn > 1:683107/1‑75 (MQ=255)
tttGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGt > 2:106735/1‑76 (MQ=255)
tCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGa > 2:521378/1‑76 (MQ=255)
gCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACAt < 1:242638/76‑1 (MQ=255)
ccAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATa < 1:299008/76‑1 (MQ=255)
ccAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATa < 2:574367/76‑1 (MQ=255)
cAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATaa < 1:399977/76‑1 (MQ=255)
cTCATTGAGGTCGCGGAGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAAccc < 2:329155/76‑1 (MQ=255)
tCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCct > 2:295303/1‑76 (MQ=255)
tGAGGTCGCGGCGAATAGTCTGCNGTCTGACNGNGAAGTGCTCCGTCGGCTCCTCGGTGCAGACGTAACCctgctg > 1:730284/1‑76 (MQ=255)
tGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCctgctg < 1:362230/76‑1 (MQ=255)
gAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGt < 2:488776/76‑1 (MQ=255)
cgcgGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGGACTGACATAACCCCGCTGTTTAAc > 2:262326/1‑76 (MQ=255)
gtgcgAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCa < 1:713523/74‑1 (MQ=255)
ggcgAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAg > 1:225140/1‑76 (MQ=255)
gAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc > 2:419512/1‑76 (MQ=255)
aaTAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg < 1:253826/76‑1 (MQ=255)
aaTAGTCGGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCTTGCTGTTTAACCAGTTCg < 2:430465/76‑1 (MQ=255)
aTAGTCTGCGGGCTGACGGGGAAGGGCTCCGCCAGCTCCTCGGGACTGACATAAACCTGCCGTTTAACCAGATCGa > 2:194951/1‑76 (MQ=255)
aTAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTAGCGACATAACCCTGCTGTTTAACCAGTTCGa < 1:770506/76‑1 (MQ=255)
tggTCTGCGGGCTGACGGAGAANTGATCCGCCANCTCCTCGGNACTGACATAAGCCTGCTGGTTANCCAGTTCGat < 2:684962/74‑1 (MQ=255)
gTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa < 1:391760/76‑1 (MQ=255)
cTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata > 2:40828/1‑76 (MQ=255)
tGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc > 2:300016/1‑76 (MQ=255)
gCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc > 2:79162/1‑76 (MQ=255)
ggAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt > 2:152944/1‑76 (MQ=255)
ggAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTGAACCAGTTCGATAATACCGTTGTGACGt > 2:708432/1‑76 (MQ=255)
agaAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg < 2:204672/76‑1 (MQ=255)
agaAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg < 1:289802/76‑1 (MQ=255)
aaGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg < 2:579529/76‑1 (MQ=255)
tGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt > 2:387407/1‑76 (MQ=255)
gCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg < 2:183819/76‑1 (MQ=255)
|
CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTG > REL606/3489055‑3489200
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
