Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A135 F1 I1 R1
|
14 |
200.0 |
12709596 |
94.6% |
12023277 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,489,199 |
T→C |
Q3R (CAA→CGA) |
glpR ← |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,489,199 | 0 | T | C | 98.9%
| 764.5
/ ‑2.3
| 184 | Q3R (CAA→CGA) | glpR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (86/95); total (88/96) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 |
Only 100 of 194 total aligned reads displayed.
TCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAA > REL606/3489127‑3489270
|
ccaccaGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgttcgttt > 1:3050046/1‑76 (MQ=255)
ccaccaGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgttcgttt > 2:5530047/1‑76 (MQ=255)
accaGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCa < 1:5942264/76‑1 (MQ=255)
caGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAtt > 2:230507/1‑76 (MQ=255)
caGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAtt < 1:5058550/76‑1 (MQ=255)
caGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAtt > 1:807596/1‑76 (MQ=255)
aGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAttt < 2:5831390/76‑1 (MQ=255)
gCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTc < 1:4002690/76‑1 (MQ=255)
ctcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCt < 1:4352025/76‑1 (MQ=255)
ctcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCt < 1:4019007/76‑1 (MQ=255)
ctcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCt < 1:6231801/76‑1 (MQ=255)
tcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTa > 2:1850060/1‑76 (MQ=255)
cctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaa > 1:1879003/1‑76 (MQ=255)
cctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaa < 1:4983582/76‑1 (MQ=255)
cctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaa < 1:5696771/76‑1 (MQ=255)
ctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaaa < 2:1174114/76‑1 (MQ=255)
ctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaaa < 1:943250/76‑1 (MQ=255)
ctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaaa > 1:456472/1‑76 (MQ=255)
tcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAAt < 1:3671258/76‑1 (MQ=255)
tcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAAt < 2:149681/76‑1 (MQ=255)
tcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTGCATTTCTAAAt < 1:2217619/76‑1 (MQ=255)
gTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATccc > 1:5637215/1‑76 (MQ=255)
gTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATccc < 1:1410866/76‑1 (MQ=255)
aCTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTg < 1:6003324/76‑1 (MQ=255)
aCTAACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTg < 1:1717127/76‑1 (MQ=255)
cTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTgg > 1:70241/1‑76 (MQ=255)
cTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTgg < 2:294862/76‑1 (MQ=255)
gACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGaa > 2:4843297/1‑76 (MQ=255)
aCATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAat < 1:1091671/76‑1 (MQ=255)
aCATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAat < 2:6178103/76‑1 (MQ=255)
cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAatt < 1:1609596/76‑1 (MQ=255)
cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAatt > 2:5804383/1‑76 (MQ=255)
cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAatt < 1:4660158/76‑1 (MQ=255)
cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTCGTGTTCGTTTCATTTCTAAATCCCTGGAatt < 2:4909395/76‑1 (MQ=255)
aaCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAattatt > 2:5256069/1‑76 (MQ=255)
ctgctgTTTAACCAGTTCGATAATACCGTTGTGACGTGGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcg < 2:44070/76‑1 (MQ=255)
gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt < 1:1209337/76‑1 (MQ=255)
gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 1:851754/1‑76 (MQ=255)
gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 1:1352024/1‑76 (MQ=255)
gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 1:2444121/1‑76 (MQ=255)
gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 2:5871600/1‑76 (MQ=255)
tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtttt < 2:4509617/76‑1 (MQ=255)
tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtttt > 1:4681325/1‑76 (MQ=255)
tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtttt > 1:6128488/1‑76 (MQ=255)
gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgttttc > 2:5980370/1‑76 (MQ=255)
gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgttttc > 1:322059/1‑76 (MQ=255)
ttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTcgc > 2:4446864/1‑76 (MQ=255)
aaCCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCgcgc < 2:4351365/76‑1 (MQ=255)
aCCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCa < 1:1002165/76‑1 (MQ=255)
ccAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCAt > 1:5335304/1‑76 (MQ=255)
aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:6182479/76‑1 (MQ=255)
aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:2273644/76‑1 (MQ=255)
aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:3201096/76‑1 (MQ=255)
aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 2:3088022/76‑1 (MQ=255)
aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:1467129/76‑1 (MQ=255)
aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 2:462009/76‑1 (MQ=255)
tCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc > 2:3517180/1‑76 (MQ=255)
ataataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa > 1:5471160/1‑76 (MQ=255)
ataataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa > 1:4929482/1‑76 (MQ=255)
taataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 2:1299982/76‑1 (MQ=255)
taataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 2:1630089/76‑1 (MQ=255)
aataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc < 1:3470224/76‑1 (MQ=255)
aataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc > 2:4235821/1‑76 (MQ=255)
aataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc > 1:2550461/1‑76 (MQ=255)
ataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca < 1:5495984/76‑1 (MQ=255)
ataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca < 1:4417708/76‑1 (MQ=255)
taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:1821849/1‑76 (MQ=255)
taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:3709876/1‑76 (MQ=255)
taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:2525761/1‑76 (MQ=255)
taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:3183275/1‑76 (MQ=255)
taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 2:2343860/1‑76 (MQ=255)
aCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac > 2:777893/1‑76 (MQ=255)
aCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac > 1:2542413/1‑76 (MQ=255)
aCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac < 1:2589176/76‑1 (MQ=255)
ccGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca < 1:5852543/76‑1 (MQ=255)
cGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa > 1:3871701/1‑76 (MQ=255)
cGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa > 2:3957767/1‑76 (MQ=255)
gTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa > 1:1992194/1‑76 (MQ=255)
gTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa < 1:4222779/76‑1 (MQ=255)
ttGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa < 2:1499047/76‑1 (MQ=255)
tgtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg < 2:1987754/76‑1 (MQ=255)
tgtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg > 1:4012323/1‑76 (MQ=255)
tgtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg > 2:3297361/1‑76 (MQ=255)
gtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc < 2:2171627/76‑1 (MQ=255)
tgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 1:3325863/76‑1 (MQ=255)
aCGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt < 2:2812520/76‑1 (MQ=255)
cGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc < 1:2151103/76‑1 (MQ=255)
gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATca < 1:4164339/76‑2 (MQ=255)
gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg > 2:5489394/1‑76 (MQ=255)
gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg > 2:1126780/1‑76 (MQ=255)
gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg < 1:3571037/76‑1 (MQ=255)
ttGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc > 2:4879042/1‑76 (MQ=255)
ttGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc > 2:5843166/1‑76 (MQ=255)
tgtgTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt > 1:5981156/1‑76 (MQ=255)
tgtgTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt > 1:6116334/1‑76 (MQ=255)
gtgtTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCGATCGCTa > 1:6250090/1‑76 (MQ=255)
gtgtTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa < 1:2739613/76‑1 (MQ=255)
gtgtTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa > 1:4052589/1‑76 (MQ=255)
tgttcgttTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa < 2:3345345/76‑1 (MQ=255)
tgttcgttTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa < 1:2407415/76‑1 (MQ=255)
|
TCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAA > REL606/3489127‑3489270
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
