Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A110 F1 I1 R1
|
14 |
278.2 |
17968888 |
92.5% |
16621221 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,268,951 |
T→G |
I129S (ATC→AGC) |
atoC → |
fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,268,951 | 0 | T | G | 99.6%
| 931.1
/ ‑5.0
| 225 | I129S (ATC→AGC) | atoC | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (112/112); total (113/112) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 |
Only 100 of 231 total aligned reads displayed.
AAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGAT > REL606/2268878‑2269023
|
aaaCCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGcc > 1:2629660/1‑76 (MQ=255)
aaCCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCg > 1:7503090/1‑76 (MQ=255)
ccGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTc < 2:3841992/76‑1 (MQ=255)
cGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCa > 2:183156/1‑76 (MQ=255)
cGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCa < 1:5815656/76‑1 (MQ=255)
tttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATc < 2:3328759/76‑1 (MQ=255)
ttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCt < 2:6208768/76‑1 (MQ=255)
ttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCt < 1:1117681/76‑1 (MQ=255)
ttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCt > 1:8685725/1‑76 (MQ=255)
tGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTg > 2:4829872/1‑76 (MQ=255)
tGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTg > 1:3571985/1‑76 (MQ=255)
gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGc < 1:7637332/76‑1 (MQ=255)
gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGc > 1:6203603/1‑76 (MQ=255)
aTCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCa > 1:1544426/1‑76 (MQ=255)
tctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAc < 2:7049610/76‑1 (MQ=255)
tctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAc > 2:218287/1‑76 (MQ=255)
ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAcc > 1:2571666/1‑76 (MQ=255)
ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAcc > 1:7178281/1‑76 (MQ=255)
ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAcc > 1:5908348/1‑76 (MQ=255)
tcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCa > 1:180832/1‑76 (MQ=255)
cGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAg > 1:8171779/1‑76 (MQ=255)
cGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAg > 2:1350787/1‑76 (MQ=255)
cGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAg < 2:4652153/76‑1 (MQ=255)
gATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAgg < 1:6701225/76‑1 (MQ=255)
gATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAgg < 1:5133705/76‑1 (MQ=255)
gATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACACCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAgg > 1:4600788/1‑76 (MQ=255)
aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc > 1:7432305/1‑76 (MQ=255)
aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc > 1:6809184/1‑76 (MQ=255)
aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc > 1:7967890/1‑76 (MQ=255)
aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc < 1:5367973/76‑1 (MQ=255)
aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc < 2:1323373/76‑1 (MQ=255)
tGNNNNGAATTTAATCGTTCAGCGCGCTTTACAACNCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCa > 2:4133173/1‑76 (MQ=255)
tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCa < 2:2913737/76‑1 (MQ=255)
tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCa < 2:8279862/76‑1 (MQ=255)
gAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCAc > 1:5482126/1‑76 (MQ=255)
gAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCAc > 1:8456238/1‑76 (MQ=255)
aGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACt > 1:6557186/1‑76 (MQ=255)
tGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAg < 2:5589722/76‑1 (MQ=255)
tttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCAcc > 1:8672382/1‑76 (MQ=255)
tttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCAcc > 2:316040/1‑76 (MQ=255)
ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCa < 2:258107/76‑1 (MQ=255)
ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCa > 2:2317087/1‑76 (MQ=255)
ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCa < 2:1276486/76‑1 (MQ=255)
tAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAg > 1:120360/1‑76 (MQ=255)
aTCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCt > 2:1138583/1‑76 (MQ=255)
cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTgg > 2:815714/1‑76 (MQ=255)
cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTgg < 2:7773869/76‑1 (MQ=255)
cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTgg > 1:165143/1‑76 (MQ=255)
ttCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCa > 1:1337281/1‑76 (MQ=255)
tccAGCGCGCTTTCCAACTCCAGTCAAGGAAAAAAAAGAGCCGTCATCTGCTCCAGGCACTGAGCACCAGCTGGCa < 2:5479041/74‑1 (MQ=255)
tCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCaa > 2:6246016/1‑76 (MQ=255)
cAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAAt < 2:8710076/76‑1 (MQ=255)
cAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAAt > 1:3022135/1‑76 (MQ=255)
gcgcgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATgg < 2:847978/76‑1 (MQ=255)
gcgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATgggg < 1:7215749/76‑1 (MQ=255)
cgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 2:8944602/76‑1 (MQ=255)
cgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 1:2880536/76‑1 (MQ=255)
cgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 2:1474186/76‑1 (MQ=255)
gcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc > 2:4295393/1‑76 (MQ=255)
cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca < 2:6588714/76‑1 (MQ=255)
cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca < 1:6585156/76‑1 (MQ=255)
cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca < 1:3924184/76‑1 (MQ=255)
cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca > 1:2673706/1‑76 (MQ=255)
tttACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac < 2:6972066/76‑1 (MQ=255)
tttACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac > 1:5851680/1‑76 (MQ=255)
tttACAACTCCAGTCAAGGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac < 1:2200078/76‑1 (MQ=255)
tACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACAt > 2:6876875/1‑76 (MQ=255)
tACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACAt > 1:6412541/1‑76 (MQ=255)
aCAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACAtt > 1:5232500/1‑76 (MQ=255)
aCAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGAACAtt > 1:7828879/1‑76 (MQ=255)
cAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtc > 2:4944852/1‑76 (MQ=255)
aaCTCCAGTGAATGAAAAAAGAGAGCCGTCTTCTGCCCCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:316040/76‑1 (MQ=255)
aaCTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:8294691/76‑1 (MQ=255)
aaCTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:2225849/76‑1 (MQ=255)
aaCCCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:4937733/76‑1 (MQ=255)
aCTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtctc < 2:8685725/76‑1 (MQ=255)
tCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCAc > 2:8355690/1‑76 (MQ=255)
ccAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCAcc > 1:974484/1‑76 (MQ=255)
aGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCaa > 1:4478700/1‑76 (MQ=255)
aGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCaa < 2:2827732/76‑1 (MQ=255)
gTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAAc < 1:3218331/76‑1 (MQ=255)
gTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAAc > 1:3745816/1‑76 (MQ=255)
tCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa < 2:5415466/76‑1 (MQ=255)
tCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa > 1:433854/1‑76 (MQ=255)
tCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa < 2:6448892/76‑1 (MQ=255)
cAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg > 1:1637801/1‑59 (MQ=255)
cAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 2:1637801/59‑1 (MQ=255)
aaTGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc < 1:6264139/76‑1 (MQ=255)
aTGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGcc > 1:2067569/1‑76 (MQ=255)
tGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGccc < 1:7317072/76‑1 (MQ=255)
gAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg < 1:6079737/76‑1 (MQ=255)
gAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg < 2:7486950/76‑1 (MQ=255)
gAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg < 2:5961939/76‑1 (MQ=255)
aaaaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCgg < 2:3441663/76‑1 (MQ=255)
aaaaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCgg > 1:4543831/1‑76 (MQ=255)
aaaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGc < 1:4822702/76‑1 (MQ=255)
aaaaGAGAGCCGTCATCTGCACGAGGCACTGAGCAACAGCTGGCAATGGGGGCACATGCTCACAAACAGCCCGTCg > 2:1887844/1‑76 (MQ=255)
aaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCg > 2:2387785/1‑76 (MQ=255)
aaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCga > 1:6785349/1‑76 (MQ=255)
agagCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCgatgat > 2:3587057/1‑76 (MQ=255)
|
AAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGAT > REL606/2268878‑2269023
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
