Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A77 F1 I1 R1
|
21 |
53.0 |
3608474 |
95.6% |
3449701 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,268,960 |
T→G |
L132R (CTG→CGG) |
atoC → |
fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,268,960 | 0 | T | G | 100.0%
| 147.8
/ NA
| 36 | L132R (CTG→CGG) | atoC | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (18/18); total (18/18) |
GATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTG > REL606/2268887‑2269032
|
gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGc < 1:1109740/76‑1 (MQ=255)
gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGc > 1:502154/1‑76 (MQ=255)
aTCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCa < 1:41028/76‑1 (MQ=255)
aTCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCa < 1:33330/76‑1 (MQ=255)
ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCAcc < 1:1237723/76‑1 (MQ=255)
tcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCa < 2:1505940/76‑1 (MQ=255)
tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCa < 2:884889/76‑1 (MQ=255)
tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCa < 2:367041/76‑1 (MQ=255)
tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCa > 1:248680/1‑76 (MQ=255)
ttGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGa > 2:796784/1‑76 (MQ=255)
ttGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGa < 1:1704114/76‑1 (MQ=255)
tGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAg < 1:323908/76‑1 (MQ=255)
gAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGc > 1:25771/1‑76 (MQ=255)
ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCa > 1:330878/1‑76 (MQ=255)
tCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTg < 1:1354982/76‑1 (MQ=255)
cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTgg > 1:613679/1‑76 (MQ=255)
ttCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCa < 2:927080/76‑1 (MQ=255)
aGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTAGCAATg > 2:1471556/1‑76 (MQ=255)
gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc < 1:1544627/76‑1 (MQ=255)
gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc < 1:1281416/76‑1 (MQ=255)
gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc > 1:153329/1‑76 (MQ=255)
gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc > 1:970668/1‑76 (MQ=255)
aaCTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 2:636941/76‑1 (MQ=255)
tCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCAc < 1:780752/76‑1 (MQ=255)
tCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa < 1:838684/76‑1 (MQ=255)
aaTGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc < 1:129844/76‑1 (MQ=255)
aaTGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc < 1:250914/76‑1 (MQ=255)
aTGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGcc < 1:1653838/76‑1 (MQ=255)
tGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGccc > 2:175147/1‑76 (MQ=255)
gAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg > 1:1744501/1‑76 (MQ=255)
aaaaGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCg > 2:655006/1‑76 (MQ=255)
agagATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCgatg < 2:365367/76‑1 (MQ=255)
tCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGa > 1:1287214/1‑76 (MQ=255)
ccGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGAc > 2:750324/1‑76 (MQ=255)
cGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGTGGCACATTCTCACCAACAGCCCGGCGATGATGGACa > 2:1257972/1‑76 (MQ=255)
cGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACa > 1:684121/1‑76 (MQ=255)
cATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCt > 2:980911/1‑76 (MQ=255)
cATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCt > 1:1421782/1‑76 (MQ=255)
aTCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTg > 1:378855/1‑76 (MQ=255)
|
GATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTG > REL606/2268887‑2269032
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
