breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 350,174 C→T A185T (GCT→ACT)  frmB ← predicted esterase
RA 474,210 Δ1 bp coding (1096/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 1,004,196 T→A intergenic (‑122/+480) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
JC JC 1,305,298 IS150 (–) +3 bp coding (940‑942/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,332,148 (T)6→7 intergenic (+131/‑79) topA → / → cysB DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,949,211 G→A Q182* (CAG→TAG)  tap ← methyl‑accepting protein IV
RA 2,141,329 Δ1 bp intergenic (‑84/+110) ECB_02035 ← / ← ECB_02036 hypothetical protein/hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,268,960 T→G L132R (CTG→CGG)  atoC → fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein
RA 3,145,769 C→T intergenic (+100/‑95) dnaG → / → rpoD DNA primase/RNA polymerase sigma factor
RA 3,613,043 +TT intergenic (‑104/+79) dctA ← / ← yhjK C4‑dicarboxylate transport protein/predicted diguanylate cyclase
RA 3,633,651 T→C E219G (GAA→GGA)  dppD ← dipeptide transporter
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,101,777 A→C intergenic (‑7/+86) hslV ← / ← ftsN ATP‑dependent protease peptidase subunit/essential cell division protein
RA 4,162,570 T→A I572N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,202,004 A→G Y186H (TAT→CAT)  iclR ← DNA‑binding transcriptional repressor
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 4217526 =49 (0.880)52 (1.030) 40/136 0.2 51.6% coding (961/2097 nt) yjbH predicted porin
?REL606 = 4440926 53 (1.050)coding (190/552 nt) yjgA hypothetical protein