breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,631,7720AC35.2% 108.7 / 17.6 54intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6063,983,7000TG35.0% 66.6 / 18.5 41T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6064,574,2520TG32.6% 79.3 / 18.4 46S5S (TCA→TCChpaXhypothetical 4‑hydroxyphenylacetate permease
*REL6061,747,9030GT32.2% 171.5 / 79.4 87A8S (GCA→TCA) ydiLhypothetical protein
*REL6061,004,1060AC32.1% 115.1 / 12.7 54intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6064,393,0610TG31.0% 112.7 / 21.7 58V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL606154,4970TG30.2% 106.9 / 12.6 53A191A (GCA→GCCyadLpredicted fimbrial‑like adhesin protein
*REL6062,296,3290AC26.9% 193.3 / 23.9 78intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6064,169,2320AT25.0% 163.0 / 14.7 72intergenic (+48/‑165)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6064,322,5650TG22.4% 184.4 / 10.6 67G157G (GGT→GGGmelBmelibiose:sodium symporter
*REL6062,417,4950TA21.8% 149.7 / 13.4 55L24F (TTA→TTTemrKEmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 379283275 (1.340)4 (0.090)
+GACTATCTTTGTTGA
4/120 5.2 6.5% coding (62/357 nt) ykgN predicted IS protein
?REL606 = 4302778 70 (1.260)coding (389/1017 nt) phnD phosphonate/organophosphate ester transporter subunit
* ? REL606 3708220 =46 (0.820)4 (0.090)
+GACTATCTTTGTTGA
4/120 5.2 8.0% coding (1247/1914 nt) mtlA fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
?REL606 = 4302778 70 (1.260)coding (389/1017 nt) phnD phosphonate/organophosphate ester transporter subunit
* ? REL606 3708220 =46 (0.820)4 (0.090)
+TCAACAAAGATAGTC
4/120 5.2 7.7% coding (1247/1914 nt) mtlA fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
?REL606 = 3792832 75 (1.340)coding (62/357 nt) ykgN predicted IS protein
* ? REL606 3708220 =46 (0.820)4 (0.090)
+GACTATCTTTGTTGA
4/120 5.2 7.7% coding (1247/1914 nt) mtlA fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
?REL606 = 3792832 75 (1.340)coding (62/357 nt) ykgN predicted IS protein
* ? REL606 1312267 =55 (0.990)4 (0.080) 4/132 5.6 7.3% intergenic (+15/+45) yciD/yciE outer membrane protein W/hypothetical protein
?REL606 1312288 = 54 (1.100)intergenic (+36/+24) yciD/yciE outer membrane protein W/hypothetical protein
* ? REL606 1520237 =81 (1.450)4 (0.090) 3/118 5.7 5.7% intergenic (‑58/+201) yddL/yddG predicted lipoprotein/predicted methyl viologen efflux pump
?REL606 1520253 = 69 (1.580)intergenic (‑74/+185) yddL/yddG predicted lipoprotein/predicted methyl viologen efflux pump
* ? REL606 = 205628756 (1.000)4 (0.080)
+CTTA
4/142 5.9 3.0% intergenic (‑191/+2) cpsG/cpsB phosphomannomutase/mannose‑1‑phosphate guanyltransferase
?REL606 4274785 = 219 (3.930)intergenic (+58/+473) gltP/yjcO glutamate/aspartate:proton symporter/hypothetical protein