breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,631,772 | 0 | A | C | 35.2% | 108.7 / 17.6 | 54 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 3,983,700 | 0 | T | G | 35.0% | 66.6 / 18.5 | 41 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 4,574,252 | 0 | T | G | 32.6% | 79.3 / 18.4 | 46 | S5S (TCA→TCC) | hpaX | hypothetical 4‑hydroxyphenylacetate permease |
* | REL606 | 1,747,903 | 0 | G | T | 32.2% | 171.5 / 79.4 | 87 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 1,004,106 | 0 | A | C | 32.1% | 115.1 / 12.7 | 54 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 4,393,061 | 0 | T | G | 31.0% | 112.7 / 21.7 | 58 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 154,497 | 0 | T | G | 30.2% | 106.9 / 12.6 | 53 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
* | REL606 | 2,296,329 | 0 | A | C | 26.9% | 193.3 / 23.9 | 78 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 4,169,232 | 0 | A | T | 25.0% | 163.0 / 14.7 | 72 | intergenic (+48/‑165) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 4,322,565 | 0 | T | G | 22.4% | 184.4 / 10.6 | 67 | G157G (GGT→GGG) | melB | melibiose:sodium symporter |
* | REL606 | 2,417,495 | 0 | T | A | 21.8% | 149.7 / 13.4 | 55 | L24F (TTA→TTT) | emrK | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 3792832 | 75 (1.340) | 4 (0.090) +GACTATCTTTGTTGA |
4/120 | 5.2 | 6.5% | coding (62/357 nt) | ykgN | predicted IS protein |
? | REL606 | = 4302778 | 70 (1.260) | coding (389/1017 nt) | phnD | phosphonate/organophosphate ester transporter subunit | |||||
* | ? | REL606 | 3708220 = | 46 (0.820) | 4 (0.090) +GACTATCTTTGTTGA |
4/120 | 5.2 | 8.0% | coding (1247/1914 nt) | mtlA | fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components |
? | REL606 | = 4302778 | 70 (1.260) | coding (389/1017 nt) | phnD | phosphonate/organophosphate ester transporter subunit | |||||
* | ? | REL606 | 3708220 = | 46 (0.820) | 4 (0.090) +TCAACAAAGATAGTC |
4/120 | 5.2 | 7.7% | coding (1247/1914 nt) | mtlA | fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components |
? | REL606 | = 3792832 | 75 (1.340) | coding (62/357 nt) | ykgN | predicted IS protein | |||||
* | ? | REL606 | 3708220 = | 46 (0.820) | 4 (0.090) +GACTATCTTTGTTGA |
4/120 | 5.2 | 7.7% | coding (1247/1914 nt) | mtlA | fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components |
? | REL606 | = 3792832 | 75 (1.340) | coding (62/357 nt) | ykgN | predicted IS protein | |||||
* | ? | REL606 | 1312267 = | 55 (0.990) | 4 (0.080) | 4/132 | 5.6 | 7.3% | intergenic (+15/+45) | yciD/yciE | outer membrane protein W/hypothetical protein |
? | REL606 | 1312288 = | 54 (1.100) | intergenic (+36/+24) | yciD/yciE | outer membrane protein W/hypothetical protein | |||||
* | ? | REL606 | 1520237 = | 81 (1.450) | 4 (0.090) | 3/118 | 5.7 | 5.7% | intergenic (‑58/+201) | yddL/yddG | predicted lipoprotein/predicted methyl viologen efflux pump |
? | REL606 | 1520253 = | 69 (1.580) | intergenic (‑74/+185) | yddL/yddG | predicted lipoprotein/predicted methyl viologen efflux pump | |||||
* | ? | REL606 | = 2056287 | 56 (1.000) | 4 (0.080) +CTTA |
4/142 | 5.9 | 3.0% | intergenic (‑191/+2) | cpsG/cpsB | phosphomannomutase/mannose‑1‑phosphate guanyltransferase |
? | REL606 | 4274785 = | 219 (3.930) | intergenic (+58/+473) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein |