Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A110 F1 I1 R1
|
14 |
278.2 |
17968888 |
92.5% |
16621221 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,326,724 |
A→G |
S15P (TCT→CCT) |
mreD ← |
cell wall structural complex MreBCD transmembrane component MreD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,326,724 | 0 | A | G | 98.8%
| 1013.3
/ 0.4
| 251 | S15P (TCT→CCT) | mreD | cell wall structural complex MreBCD transmembrane component MreD |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (137/111); total (138/113) |
| Fisher's exact test for biased strand distribution p-value = 5.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01 |
Only 100 of 272 total aligned reads displayed.
ACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTT > REL606/3326651‑3326797
|
acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGACaggga > 1:2125917/1‑76 (MQ=255)
acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 2:848508/76‑1 (MQ=255)
acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 2:2781974/76‑1 (MQ=255)
acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 2:6956901/76‑1 (MQ=255)
acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 1:3454891/76‑1 (MQ=255)
cccAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAagggag < 2:3662796/76‑1 (MQ=255)
cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGcc > 1:6848734/1‑76 (MQ=255)
cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGcc > 1:8032336/1‑76 (MQ=255)
cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGcc < 1:8874926/76‑1 (MQ=255)
gTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAg < 1:3205770/76‑1 (MQ=255)
gTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAg > 1:8403443/1‑76 (MQ=255)
tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGa > 2:5509054/1‑76 (MQ=255)
tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGa < 2:5955816/76‑1 (MQ=255)
tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGa > 2:1927607/1‑76 (MQ=255)
ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt > 1:463357/1‑76 (MQ=255)
ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt < 1:5885943/76‑1 (MQ=255)
ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt < 2:8222072/76‑1 (MQ=255)
ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt > 2:7098853/1‑76 (MQ=255)
ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt < 2:3184905/76‑1 (MQ=255)
tGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAtt > 1:2155015/1‑76 (MQ=255)
ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTa > 2:7845775/1‑76 (MQ=255)
ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTa > 2:5092718/1‑76 (MQ=255)
ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTa < 1:7034313/76‑1 (MQ=255)
gCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTAc < 1:8531951/76‑1 (MQ=255)
ggAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCa < 1:5586544/76‑1 (MQ=255)
gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAg < 2:2998952/76‑1 (MQ=255)
gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAg > 1:1920687/1‑76 (MQ=255)
aaaaCAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGc > 2:1081428/1‑76 (MQ=255)
aaaCAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCCATGAGGAAAGGGAGCCAGATTACCCAGCg > 2:2684030/1‑76 (MQ=255)
cAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTcc < 2:1226132/76‑1 (MQ=255)
cAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTcc > 1:1091085/1‑76 (MQ=255)
aTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCt < 1:1496730/76‑1 (MQ=255)
aTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCt > 1:7379414/1‑76 (MQ=255)
aTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCt < 1:6965582/76‑1 (MQ=255)
tCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTg < 2:662395/76‑1 (MQ=255)
cAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTgg > 1:2867018/1‑76 (MQ=255)
cAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTgg > 1:6671108/1‑76 (MQ=255)
ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCt < 2:5016740/76‑1 (MQ=255)
gTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTa < 2:8895418/76‑1 (MQ=255)
gTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTa < 2:6765198/76‑1 (MQ=255)
ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCTTGGCTAc > 1:5729497/1‑76 (MQ=255)
ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTAc < 2:7767748/76‑1 (MQ=255)
ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTAc < 1:3047978/76‑1 (MQ=255)
ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTAc > 2:8739795/1‑76 (MQ=255)
tATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACg < 2:6229096/76‑1 (MQ=255)
aTCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGa > 2:6475195/1‑76 (MQ=255)
tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCGGGCTACGAt < 2:2118480/76‑1 (MQ=255)
ccGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATa > 1:4523728/1‑76 (MQ=255)
cGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAg > 1:6747228/1‑76 (MQ=255)
cGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAg > 1:7882632/1‑76 (MQ=255)
gCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCt > 1:6600647/1‑76 (MQ=255)
cAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTcc > 2:8040173/1‑75 (MQ=255)
aGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGc > 1:8350613/1‑76 (MQ=255)
gCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCAc > 2:6108610/1‑76 (MQ=255)
gCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCAc > 1:7180946/1‑76 (MQ=255)
cATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACt > 2:4826802/1‑76 (MQ=255)
aTGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTTGCCACTa > 2:7187443/1‑76 (MQ=255)
aTGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCCCTa > 1:1773730/1‑76 (MQ=255)
aTGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTa > 2:4793232/1‑76 (MQ=255)
gATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAtt > 2:2731566/1‑76 (MQ=255)
gATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAtt < 2:3987467/76‑1 (MQ=255)
gATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAtt < 1:6827454/76‑1 (MQ=255)
aTTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTg < 2:2313583/76‑1 (MQ=255)
aTTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTg < 2:186/76‑1 (MQ=255)
aTTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTg > 1:6392165/1‑76 (MQ=255)
tttGCAGAAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACCATAGCTCGCCACTATTGc > 2:7479917/1‑76 (MQ=255)
tGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGccc > 2:3593974/1‑76 (MQ=255)
tGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGccc < 2:4362295/76‑1 (MQ=255)
cggcaACAGCGCAAGGAGGAAAGGGAGCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTc < 1:599764/74‑1 (MQ=255)
cagcaACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTc < 2:3098944/76‑1 (MQ=255)
gcaACAGCGCAATGAGGAAAGGGAGCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTccc < 1:3581958/76‑1 (MQ=255)
gcaACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTccc > 1:492003/1‑76 (MQ=255)
caACAGCGCAATGAGGAAAGGGAGCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCg < 1:1331775/76‑1 (MQ=255)
caACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCg < 2:1468276/76‑1 (MQ=255)
aaCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCgg > 1:4187216/1‑76 (MQ=255)
aaCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCgg > 2:8420241/1‑76 (MQ=255)
aaCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCgg < 1:1061663/76‑1 (MQ=255)
aCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgc < 1:5336512/76‑1 (MQ=255)
cAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgcg > 1:6564168/1‑76 (MQ=255)
aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgcgc > 1:2726370/1‑76 (MQ=255)
aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgcgc < 2:34438/76‑1 (MQ=255)
aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCCgcgc > 1:509027/1‑76 (MQ=255)
aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCATGGCTACGATAGCTCGCCACGATTGCCCTCCCGgcgc > 1:8933470/1‑76 (MQ=255)
gcgcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCa < 2:5226568/76‑1 (MQ=255)
gcgcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCa > 1:6243546/1‑76 (MQ=255)
cgcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCAc > 1:8191041/1‑76 (MQ=255)
gcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCAcg > 1:7709948/1‑76 (MQ=255)
cAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCAcgc < 1:1285676/76‑1 (MQ=255)
aaTGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCCTcccgcgcccgcg > 2:1623649/1‑76 (MQ=255)
aggAGGAAGGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACgcgc < 1:42758/74‑1 (MQ=255)
aTGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACgcgc > 1:4803383/1‑76 (MQ=255)
aTGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCCCCCGG‑GCAcgccaa > 1:3802591/1‑73 (MQ=255)
tGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCa < 1:5249776/76‑1 (MQ=255)
tGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCa < 1:7944484/76‑1 (MQ=255)
gAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAg < 1:873430/76‑1 (MQ=255)
gAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAg < 2:6670475/76‑1 (MQ=255)
aGGAAAGGGACCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAgg < 2:2535304/76‑1 (MQ=255)
gAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCg > 1:755942/1‑76 (MQ=255)
aaaGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCgg > 1:2626411/1‑76 (MQ=255)
agagagCCAGCTTCCCCAGCGGCCCTGGCTACAATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGtt < 1:6916355/76‑1 (MQ=255)
|
ACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTT > REL606/3326651‑3326797
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
