Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A48 F1 I1 R1
|
19 |
65.6 |
4133022 |
97.0% |
4009031 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,959,951 |
C→T |
A44A (GCC→GCT) |
uvrD → |
DNA‑dependent ATPase I and helicase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,959,951 | 0 | C | T | 100.0%
| 224.1
/ NA
| 57 | A44A (GCC→GCT) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/39); total (18/39) |
CGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCG > REL606/3959883‑3960020
|
cGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCtga < 2:1662213/76‑1 (MQ=255)
cGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCtga < 2:136413/76‑1 (MQ=255)
cGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCtga > 2:1147306/1‑76 (MQ=255)
cGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCtga > 1:1865583/1‑76 (MQ=255)
cagcaACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCtgatg < 1:1933046/76‑1 (MQ=255)
caacaACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCtgatg > 2:220285/4‑76 (MQ=255)
gcaACCTTCTGGTGCTGGCGGGCGCGGGCTGTGGTAAGACTCGCGTACTGGTGGATCGTATCGCTTGGCtgatggg > 2:951705/1‑74 (MQ=38)
aaCCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGGGCg > 2:914574/1‑76 (MQ=255)
aaCCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCg > 2:482565/1‑76 (MQ=255)
aaCCTTCTGGGGCTGGCGGGCGCGGGCAGAGGTAAGACGCGCGTCCTGGTGCATCGTATCGTTTGGGTGCTGAGCg > 2:218070/1‑76 (MQ=255)
aCCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGt > 2:1618268/1‑76 (MQ=255)
aCCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGt > 1:1643756/1‑76 (MQ=255)
cgttCTGGTGCGGGGGGGCGGGGGCAGTGGTAAGACGTGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTg < 2:520854/74‑1 (MQ=255)
ggggTGCGGGGGGGCGGGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGaaa < 2:990741/74‑1 (MQ=255)
cTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGaaa < 2:65075/76‑1 (MQ=255)
ggggggcgggggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGctct < 2:799131/73‑1 (MQ=255)
ggcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCAt < 1:1829872/76‑1 (MQ=255)
gcgggggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATa < 1:1268200/76‑1 (MQ=255)
gcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATa < 1:1306344/76‑1 (MQ=255)
gcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATa < 2:306773/76‑1 (MQ=255)
ggggggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATAc < 1:910921/73‑1 (MQ=255)
gggggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACt < 2:320116/74‑1 (MQ=255)
gcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACt < 1:2064645/76‑1 (MQ=255)
cgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTc > 2:286983/1‑76 (MQ=255)
gggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCg < 1:100229/76‑1 (MQ=255)
gcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGAt > 2:1617129/1‑76 (MQ=255)
cAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGAtt < 1:1367035/76‑1 (MQ=255)
aGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTa < 2:365329/76‑1 (MQ=255)
aGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTa < 2:858338/76‑1 (MQ=255)
gTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTAt < 1:1251065/76‑1 (MQ=255)
gTGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTAt < 2:1269850/76‑1 (MQ=255)
tGGTAAGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATg > 2:1311588/1‑76 (MQ=255)
aGACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCggg > 1:1545832/1‑75 (MQ=255)
gACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTg < 2:851994/76‑1 (MQ=255)
gACGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTg < 2:1848942/76‑1 (MQ=255)
aCGCGCGTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGa < 1:568530/76‑1 (MQ=255)
cgcgcgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGAc < 1:502279/76‑1 (MQ=255)
cgcgcgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGAc < 2:1952827/76‑1 (MQ=255)
cgcgcgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGAc < 1:703766/76‑1 (MQ=255)
cgcgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGGGACGt > 2:2025557/1‑76 (MQ=255)
gcgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGtt < 1:1455004/76‑1 (MQ=255)
gcgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGtt < 2:503517/76‑1 (MQ=255)
cgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGttt < 2:1142392/76‑1 (MQ=255)
cgTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGGGACGctt > 1:905925/1‑76 (MQ=255)
gTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGAGGTTTa > 1:1132691/1‑76 (MQ=255)
gTACTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGGGGCGtttt > 1:1197379/1‑75 (MQ=255)
ttcTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTAc < 1:1582537/74‑1 (MQ=255)
cTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGAGGTTTACca > 1:29946/1‑76 (MQ=255)
cTGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACca < 1:1914809/76‑1 (MQ=255)
tGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACcaa < 2:1046871/76‑1 (MQ=255)
tGGTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACGCGATTATGGCGGTGACGTTTACcaa < 1:1324055/76‑1 (MQ=255)
ggTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACcaaa > 1:1998661/1‑75 (MQ=255)
gTGCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACcaaca < 2:24387/76‑1 (MQ=255)
gCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACaaa < 1:1259766/76‑1 (MQ=255)
gCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACaaa < 1:298885/76‑1 (MQ=255)
gCATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACaaa < 1:130369/76‑1 (MQ=255)
cATCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTTCCAACaaaa > 2:779833/1‑75 (MQ=255)
aTCGTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgc < 2:1899215/76‑1 (MQ=255)
gTATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggc < 1:866268/76‑1 (MQ=255)
tATCGCTTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggcg > 2:756062/1‑76 (MQ=255)
|
CGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGCTGATGAGCGTGGAAAACTGCTCTCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCG > REL606/3959883‑3960020
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
