Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A126 F1 I1 R1
|
14 |
223.8 |
14402144 |
95.1% |
13696438 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,333,651 |
G→A |
S400F (TCT→TTT) |
nuoN ← |
NADH dehydrogenase subunit N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,333,651 | 0 | G | A | 99.5%
| 766.3
/ NA
| 187 | S400F (TCT→TTT) | nuoN | NADH dehydrogenase subunit N |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (76/110); minor base C (1/0); total (77/110) |
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 202 total aligned reads displayed.
CAGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGG > REL606/2333578‑2333723
|
cAGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCGCCAGTACCAACAGTGCaaaa < 1:6218261/76‑1 (MQ=255)
cAGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCaaaa > 1:3860457/1‑76 (MQ=255)
cAGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCaaaa > 1:4501961/1‑76 (MQ=255)
cAGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCaaaa > 1:2119359/1‑76 (MQ=255)
aGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAaaaa > 1:3401939/1‑76 (MQ=255)
cGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATc > 2:76985/1‑76 (MQ=255)
cGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATc > 2:7111910/1‑76 (MQ=255)
ggCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCa < 2:4647003/76‑1 (MQ=255)
aTTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGca > 2:2251905/1‑76 (MQ=255)
aTTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGca > 2:1966418/1‑76 (MQ=255)
aTTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGca > 2:3514546/1‑76 (MQ=255)
aTTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGca > 2:3814079/1‑76 (MQ=255)
ttaccAAACGCACAAAACTAATCAGAGGTTGTCGCCAAACACCCAGGACCAGTACCAACAGTGCAAAAATCTGcac > 2:3488618/4‑76 (MQ=255)
tGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATAGACCCAGCACCAGTACCAACAGTGCAAAACTCAGcacc > 1:5015834/1‑76 (MQ=255)
tGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGcacc > 1:755338/1‑76 (MQ=255)
tGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGcacc < 1:3017334/76‑1 (MQ=255)
gCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGcacca < 1:1075669/76‑1 (MQ=255)
cAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACg < 2:3137468/76‑1 (MQ=255)
cAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACg < 1:965600/76‑1 (MQ=255)
aaCGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGAt < 1:5971639/76‑1 (MQ=255)
aCGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATa < 2:6302099/76‑1 (MQ=255)
cacaATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccg > 1:5723387/1‑76 (MQ=255)
cacaATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccg > 1:4183914/1‑76 (MQ=255)
cacaATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccg > 2:6285679/1‑76 (MQ=255)
cacaATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccg > 1:2185802/1‑76 (MQ=255)
acaATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccgc > 2:3909723/1‑76 (MQ=255)
aaTACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccgccc > 1:5463660/1‑76 (MQ=255)
aaTACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccgccc < 1:6042178/76‑1 (MQ=255)
aTACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccgcccg < 2:7196928/76‑1 (MQ=255)
aTACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccgcccg < 1:4609610/76‑1 (MQ=255)
tACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATAccgcccgc > 1:2934681/1‑76 (MQ=255)
aCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCcgcg < 2:5487241/76‑1 (MQ=255)
aCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCcgcg < 1:5178635/76‑1 (MQ=255)
cTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCgcgc > 2:5150326/1‑76 (MQ=255)
tAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCt < 1:5834160/76‑1 (MQ=255)
aaTCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTg < 2:6744051/76‑1 (MQ=255)
tCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTa > 1:3423544/1‑76 (MQ=255)
tCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTa > 2:2428612/1‑76 (MQ=255)
tCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTa > 2:4275201/1‑76 (MQ=255)
cAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTAc < 1:973508/76‑1 (MQ=255)
gCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTg < 2:3204974/76‑1 (MQ=255)
gCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTg < 1:44778/76‑1 (MQ=255)
gCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTg < 1:2427454/76‑1 (MQ=255)
cGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGc > 1:1835385/1‑76 (MQ=255)
cGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGc < 1:1516999/76‑1 (MQ=255)
cGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGc > 1:7103837/1‑76 (MQ=255)
cGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGGACTGc > 2:6031971/1‑76 (MQ=255)
ggTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGcc < 2:2699206/76‑1 (MQ=255)
ggTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGcc < 2:5362170/76‑1 (MQ=255)
ggTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGcc < 2:1853023/76‑1 (MQ=255)
gTTGTGGCCATACACCCAGCACCAGTACCACCAGTGCACAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCa > 2:3576558/1‑76 (MQ=255)
gTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCa < 1:3331781/76‑1 (MQ=255)
gTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCa < 1:446097/76‑1 (MQ=255)
gTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCa < 2:460418/76‑1 (MQ=255)
tgtgGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGt > 1:3485329/1‑76 (MQ=255)
gtgGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGtt > 1:3165004/1‑76 (MQ=255)
tgGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGttt > 1:5625474/1‑76 (MQ=255)
tgGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGttt < 2:6628476/76‑1 (MQ=255)
tgGCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGttt < 2:3091847/76‑1 (MQ=255)
ggCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTg < 1:3701936/76‑1 (MQ=255)
ggCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTg < 1:6306034/76‑1 (MQ=255)
ggCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTg > 1:147506/1‑76 (MQ=255)
ggCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTg < 1:4986430/76‑1 (MQ=255)
ggCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTg < 2:2795312/76‑1 (MQ=255)
ggCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTg < 1:1655835/76‑1 (MQ=255)
gCCATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGa < 1:186869/76‑1 (MQ=255)
cATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATg < 2:4633787/76‑1 (MQ=255)
cATACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATg < 2:5903314/76‑1 (MQ=255)
aTACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATgg < 1:4025476/76‑1 (MQ=255)
aTACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATgg < 1:7124169/76‑1 (MQ=255)
aTACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATgg < 1:2759321/76‑1 (MQ=255)
tACACCCAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGt < 1:1048036/76‑1 (MQ=255)
cccAGCACCAGTACCAACAGTGCAAAGATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCAt > 1:4769928/1‑76 (MQ=255)
cccAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCAt > 2:2262741/1‑76 (MQ=255)
cAGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATcg < 2:5703007/76‑1 (MQ=255)
aGCACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCAgcgc > 1:5418243/1‑76 (MQ=255)
cACCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGa > 1:3655441/1‑76 (MQ=255)
aCCAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGAc < 1:4142781/76‑1 (MQ=255)
ccAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGccc > 2:1986833/1‑76 (MQ=255)
ccAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGAcc < 2:4357836/76‑1 (MQ=255)
ccAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGAcc > 2:6901206/1‑76 (MQ=255)
cAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGAccc < 2:3008249/76‑1 (MQ=255)
cAGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGAccc < 1:1197656/76‑1 (MQ=255)
aGTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCg < 1:605157/76‑1 (MQ=255)
gTACCAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCgg < 1:4700365/76‑1 (MQ=255)
ccAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGgttg > 2:2193372/1‑76 (MQ=255)
ccAACAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGgttg > 1:2216473/1‑76 (MQ=255)
aCAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTc < 2:126578/76‑1 (MQ=255)
aCAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTc < 1:6523323/76‑1 (MQ=255)
aCAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTc < 1:4287358/76‑1 (MQ=255)
cAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTcc < 2:574194/76‑1 (MQ=255)
cAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTcc > 1:3755201/1‑76 (MQ=255)
cAGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTcc < 1:3925474/76‑1 (MQ=255)
aGTGCAAAAATCAGCCCCACGATACCGCCCGCGCTGTACTGCCAGTTTGGTGGTGCATCGCGACCCGGTTGTTCCg < 1:5700393/76‑1 (MQ=255)
aGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCg < 1:1710103/76‑1 (MQ=255)
aGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCg < 1:4398280/76‑1 (MQ=255)
aGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCg < 2:1463283/76‑1 (MQ=255)
aGTGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCg > 2:4724444/1‑76 (MQ=255)
tGCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCggg < 2:7047029/76‑1 (MQ=255)
gCAAAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCgggg < 2:5959903/76‑1 (MQ=255)
|
CAGCGGCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAAATCAGCACCACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGG > REL606/2333578‑2333723
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
