Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A83 F1 I1 R1
|
17 |
39.7 |
2446701 |
98.6% |
2412447 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,336,054 |
G→A |
A171V (GCA→GTA) |
nuoM ← |
NADH dehydrogenase subunit M |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,336,054 | 0 | G | A | 100.0%
| 81.9
/ NA
| 23 | A171V (GCA→GTA) | nuoM | NADH dehydrogenase subunit M |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/13); total (10/13) |
ACCAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAC > REL606/2335988‑2336124
|
aCCAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGa > 1:839882/1‑76 (MQ=255)
ccAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGAt > 2:221810/1‑76 (MQ=255)
ccAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGAt < 1:843705/76‑1 (MQ=255)
ggATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGtttt < 2:823789/76‑1 (MQ=255)
aTGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTAc > 1:1136531/1‑76 (MQ=255)
aTGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTAc > 2:247468/1‑76 (MQ=255)
ggCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCg < 1:1191843/76‑1 (MQ=255)
cATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCt < 1:128294/76‑1 (MQ=255)
aCCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt < 2:66801/76‑1 (MQ=255)
gACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGccc < 1:1110794/76‑1 (MQ=255)
cACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCca < 1:343265/76‑1 (MQ=255)
aCTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcac < 1:963887/76‑1 (MQ=255)
tCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg < 2:931968/76‑1 (MQ=255)
cGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt > 2:55104/1‑76 (MQ=255)
ccTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGc < 1:346175/76‑1 (MQ=255)
tgtAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg < 2:1206013/76‑1 (MQ=255)
tgtAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg > 1:58908/1‑76 (MQ=255)
gtAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg > 1:616277/1‑76 (MQ=255)
tAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa > 2:367583/1‑76 (MQ=255)
aaTGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg > 1:616213/1‑76 (MQ=255)
agaaCTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACAt < 2:484522/76‑1 (MQ=255)
aCTTGGTTACCGCCGTGATACGCGTTTTACCGTTAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCgg < 2:579119/76‑1 (MQ=255)
aCTTGGTTACCGCCGTGATAAGCGTTTTAACGTCAGAGGCTTTATGCCAAAACAGTGAGATCAGGAAGTACATCgg > 1:988647/1‑76 (MQ=255)
tGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATAAGGAAGAACATCGGCAc > 1:173764/1‑76 (MQ=255)
|
ACCAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAC > REL606/2335988‑2336124
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
