Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A75 F1 I1 R1
|
16 |
50.8 |
3316832 |
95.7% |
3174208 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,610,811 |
C→T |
P329P (CCG→CCA) |
yfhK ← |
predicted sensory kinase in two‑component system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,610,811 | 0 | C | T | 100.0%
| 205.4
/ NA
| 52 | P329P (CCG→CCA) | yfhK | predicted sensory kinase in two‑component system |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/29); total (23/29) |
TGAGATCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGCGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAGTTGT > REL606/2610738‑2610880
|
tGAGATCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTgg > 2:832603/1‑76 (MQ=255)
agaTCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAgtggtg < 2:204912/76‑1 (MQ=255)
gaTCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGc > 2:911733/1‑76 (MQ=255)
aTCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCt > 2:1643741/1‑76 (MQ=255)
aTCGACGTCGGTACGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCt < 1:803970/76‑1 (MQ=255)
cGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTaa > 2:1578112/1‑76 (MQ=255)
gTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCa > 1:981846/1‑76 (MQ=255)
gCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATtctc > 1:1103419/1‑76 (MQ=255)
catcatTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTcc < 2:826596/76‑1 (MQ=255)
tttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTcc < 1:1284874/76‑1 (MQ=255)
tttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTcc > 2:891669/1‑76 (MQ=255)
tttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTcc > 1:817474/1‑76 (MQ=255)
ttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCa > 1:1116980/1‑76 (MQ=255)
tAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCAc > 1:1241379/1‑76 (MQ=255)
gTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACt > 2:1100175/1‑76 (MQ=255)
cAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGcc > 2:996858/1‑76 (MQ=255)
ggCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTg < 2:799627/76‑1 (MQ=255)
ggCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTg < 2:464507/76‑1 (MQ=255)
cTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGtt > 1:974427/1‑76 (MQ=255)
cTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTCtt > 1:1282062/1‑76 (MQ=255)
tATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGttt < 2:275369/76‑1 (MQ=255)
tATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGttt < 2:1580841/76‑1 (MQ=255)
aTGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTa > 1:748176/1‑76 (MQ=255)
aTGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTa > 2:732890/1‑76 (MQ=255)
tGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTAc < 2:571201/76‑1 (MQ=255)
gAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACg > 1:1273032/1‑76 (MQ=255)
gAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACg > 1:174117/1‑76 (MQ=255)
gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt < 1:287351/76‑1 (MQ=255)
gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt < 1:974244/76‑1 (MQ=255)
gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt < 1:1596466/76‑1 (MQ=255)
gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt < 1:3419/76‑1 (MQ=255)
aGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTg < 2:347673/76‑1 (MQ=255)
aaaCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTa > 2:1496662/1‑76 (MQ=255)
aaaCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTa > 2:891977/1‑76 (MQ=255)
aaCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTaa < 1:147240/76‑1 (MQ=255)
aCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAAt < 1:456017/76‑1 (MQ=255)
ccACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATc < 2:1404969/76‑1 (MQ=255)
ccACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATc > 2:707526/1‑76 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:895333/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:465632/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:709362/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:137673/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:791308/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:367404/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 2:499491/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:93289/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:937441/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:321120/76‑1 (MQ=255)
cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg < 1:986879/76‑1 (MQ=255)
tGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGc < 2:804620/76‑1 (MQ=255)
ccaccaGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAgttg > 2:333955/1‑76 (MQ=255)
ccaccaGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAgttg > 2:1529965/1‑76 (MQ=255)
caccaGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAgttgt < 1:1595386/76‑1 (MQ=255)
|
TGAGATCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGCGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAGTTGT > REL606/2610738‑2610880
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
