Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A41 F1 I1 R1
|
21 |
57.1 |
3643130 |
97.0% |
3533836 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,616,302 |
(G)7→6 |
intergenic (‑42/‑354) |
purL ← / → yfhD |
phosphoribosylformylglycinamidine synthase/putative periplasmic binding protein of transport system; b2558_1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,616,296 | 0 | G | . | 94.7%
| 259.7
/ 8.5
| 57 | intergenic (‑36/‑360) | purL/yfhD | phosphoribosylformylglycinamidine synthase/putative periplasmic binding protein of transport system; b2558_1 |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base . (31/23); total (32/25) |
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 |
ATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACC > REL606/2616221‑2616367
|
aTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAg < 2:939039/76‑1 (MQ=255)
aTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAg > 1:1367557/1‑76 (MQ=255)
aTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAg < 1:932048/76‑1 (MQ=255)
tcccGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgg < 1:727272/74‑1 (MQ=255)
tGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgg > 1:955807/1‑76 (MQ=255)
tGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgg > 2:357562/1‑76 (MQ=255)
gCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAggg < 1:438024/76‑1 (MQ=255)
gACCGTGCCGGCGAACCCCGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgggggg < 1:751214/76‑1 (MQ=255)
aGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGaaa < 1:1528478/76‑1 (MQ=255)
gTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGaaac > 1:1627434/1‑75 (MQ=255)
gTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGaaac < 1:240499/76‑2 (MQ=255)
tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTTCCCAA‑GGGGGGAAACg < 2:1147057/76‑1 (MQ=255)
tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACg > 1:1166773/1‑76 (MQ=255)
tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACg > 1:755363/1‑76 (MQ=255)
tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACg < 2:1267318/76‑1 (MQ=255)
gCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACgg < 1:1525080/76‑1 (MQ=255)
cAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACggg > 2:599031/1‑76 (MQ=255)
aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGc < 2:145847/76‑1 (MQ=255)
aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGc > 2:587216/1‑76 (MQ=255)
gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGt > 2:337160/1‑76 (MQ=255)
gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGt < 1:213505/76‑1 (MQ=255)
gAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTCCCCAA‑GGGGGGAAACGGGCGTCa < 2:682530/76‑1 (MQ=255)
gAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCa < 2:981392/76‑1 (MQ=255)
gAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCa < 2:994179/76‑1 (MQ=255)
aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCAt > 1:1241639/1‑76 (MQ=255)
cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATtat < 1:497144/76‑1 (MQ=255)
cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATtat < 1:990117/76‑1 (MQ=255)
gCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATaaa < 1:1024749/76‑1 (MQ=255)
gCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATaaa > 2:1776358/1‑76 (MQ=255)
cAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAg < 2:1690769/76‑1 (MQ=255)
aaTTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAAt > 1:667761/1‑76 (MQ=255)
tttCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCt < 1:907931/76‑1 (MQ=255)
ttCAATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTg > 2:1622661/1‑76 (MQ=255)
atcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATgc > 1:826071/1‑76 (MQ=255)
catAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCCTTATAAAGAATCTGATgcgc > 2:416324/1‑76 (MQ=255)
atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCt > 2:1430265/1‑76 (MQ=255)
tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTg > 2:1429051/1‑76 (MQ=255)
ctctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaa > 2:1087924/1‑76 (MQ=255)
ctctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaa < 2:1347823/76‑1 (MQ=255)
ctctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaa < 2:344122/76‑1 (MQ=255)
ctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc > 1:198865/1‑76 (MQ=255)
ctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc > 2:1812753/1‑76 (MQ=255)
tcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAcc < 2:361240/76‑1 (MQ=255)
cGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCg > 2:263612/1‑76 (MQ=255)
cGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCg > 1:1821524/1‑76 (MQ=255)
gTCTTCTTAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGt > 2:1138890/1‑76 (MQ=255)
gTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGt > 2:1256329/1‑76 (MQ=255)
tcttctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGtt < 2:762550/76‑1 (MQ=255)
tcttctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGtt > 1:1444216/1‑76 (MQ=255)
tctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGc > 1:363225/1‑76 (MQ=255)
ctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg > 1:934636/1‑76 (MQ=255)
ctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg > 1:1214928/1‑76 (MQ=255)
aaGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg < 2:1719814/76‑1 (MQ=255)
aaGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg < 2:307974/76‑1 (MQ=255)
aGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTgg < 2:355449/76‑1 (MQ=255)
cTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa < 1:335422/76‑1 (MQ=255)
cTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa > 1:968863/1‑76 (MQ=255)
tttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaaa > 1:900819/1‑76 (MQ=255)
tttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaaa > 1:822967/1‑76 (MQ=255)
ttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAAt > 1:652521/1‑76 (MQ=255)
ttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAAt < 1:1187957/76‑1 (MQ=255)
tgtACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAAt > 2:1297074/1‑76 (MQ=255)
tgtACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAAt < 1:750788/76‑1 (MQ=255)
tACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCa > 2:471053/1‑76 (MQ=255)
cccAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCAcc > 1:312352/1‑76 (MQ=255)
|
ATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACC > REL606/2616221‑2616367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
