Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A75 F1 I1 R1
|
16 |
50.8 |
3316832 |
95.7% |
3174208 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,028,245 |
Δ1 bp |
coding (217/777 nt) |
ECB_02828 ← |
Polysialic acid transport protein kpsM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,028,243 | 0 | G | . | 93.1%
| 232.8
/ 18.0
| 61 | coding (219/777 nt) | ECB_02828 | Polysialic acid transport protein kpsM |
| Reads supporting (aligned to +/- strand): ref base G (1/2); major base . (25/29); minor base A (4/0); total (30/31) |
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 |
CGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCAAA > REL606/3028169‑3028314
|
cGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAgg > 1:1120431/1‑76 (MQ=255)
cGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAgg > 1:112766/1‑76 (MQ=255)
aTAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAggaa > 1:1315365/1‑74 (MQ=255)
aTTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATc < 1:199607/76‑1 (MQ=255)
aTTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCa < 1:1196315/76‑1 (MQ=255)
ttAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAg > 1:237962/1‑76 (MQ=255)
ttAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAg > 1:268822/1‑76 (MQ=255)
ttAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAg > 1:945075/1‑76 (MQ=255)
aaaCAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAgg > 2:260425/1‑76 (MQ=255)
acaacCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCa < 2:1057293/76‑1 (MQ=255)
caacCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCAt > 2:1244119/1‑76 (MQ=255)
aacCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCAtt > 2:1409684/1‑76 (MQ=255)
acCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTa < 1:580464/76‑1 (MQ=255)
ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa < 2:1184870/76‑1 (MQ=255)
ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:127217/1‑76 (MQ=255)
ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:1406557/1‑76 (MQ=255)
ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:1443498/1‑76 (MQ=255)
ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:996889/1‑76 (MQ=255)
ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa < 1:1296459/76‑1 (MQ=255)
ccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGt < 2:497249/76‑1 (MQ=255)
cTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTa < 2:704044/76‑1 (MQ=255)
gATTCGCTTCAATAGCACCTATAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTaaa > 1:811991/1‑76 (MQ=255)
ttCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTaaaaa < 1:51943/76‑1 (MQ=255)
gCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAacac < 1:1086857/76‑1 (MQ=255)
ttCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCg > 1:1573921/1‑76 (MQ=255)
cAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAGGCCATTAAGTAAAAACACCgg < 1:137524/76‑1 (MQ=255)
cAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCggg > 1:352426/1‑76 (MQ=255)
aTAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGaa < 2:1250476/76‑1 (MQ=255)
tAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAAt < 2:333109/76‑1 (MQ=255)
aGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATg < 2:743149/76‑1 (MQ=255)
aGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATg < 1:553512/76‑1 (MQ=255)
gCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGaa > 1:681911/1‑73 (MQ=255)
gCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGaa < 2:681911/73‑1 (MQ=255)
aCCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATgaga > 1:1512532/1‑76 (MQ=255)
cTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATg < 2:1606243/76‑1 (MQ=255)
cTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATg < 2:959383/76‑1 (MQ=255)
aCAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTc > 2:1483515/1‑76 (MQ=255)
cAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCt > 2:1148798/1‑76 (MQ=255)
aGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTg < 2:785099/76‑1 (MQ=255)
aaCGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGc < 1:1267563/76‑1 (MQ=255)
aaCGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGc < 1:19557/76‑1 (MQ=255)
aaCGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGc < 1:224411/76‑1 (MQ=255)
cGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCAt > 2:1237195/1‑76 (MQ=255)
tGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTg < 2:789332/76‑1 (MQ=255)
tGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTg < 2:246124/76‑1 (MQ=255)
tGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTg > 1:1117560/1‑76 (MQ=255)
tGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCgg > 1:1095376/1‑76 (MQ=255)
tACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGc < 1:455642/76‑1 (MQ=255)
tGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTATAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATa < 2:1421117/76‑1 (MQ=255)
tGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATa < 1:1072184/76‑1 (MQ=255)
cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGcatcat > 1:272899/1‑76 (MQ=255)
cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAAt > 1:272914/1‑76 (MQ=255)
cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAAt > 1:1198994/1‑76 (MQ=255)
cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAAt < 2:613476/76‑1 (MQ=255)
tAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATg < 2:55636/76‑1 (MQ=255)
aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt > 2:537796/1‑76 (MQ=255)
aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt < 2:268822/76‑1 (MQ=255)
aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt > 2:1534424/1‑76 (MQ=255)
aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt > 2:1115741/1‑76 (MQ=255)
aGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTaa < 2:127363/76‑1 (MQ=255)
ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 2:462068/74‑1 (MQ=255)
ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:1054805/74‑1 (MQ=255)
ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:1647008/74‑1 (MQ=255)
ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:1054814/74‑1 (MQ=255)
ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:524131/74‑1 (MQ=255)
ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc > 1:825842/3‑76 (MQ=255)
taaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCa < 1:433079/75‑1 (MQ=255)
taaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCa > 2:779789/2‑76 (MQ=255)
aaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaa > 1:524043/1‑76 (MQ=255)
aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:689339/1‑76 (MQ=255)
aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:640516/1‑76 (MQ=255)
aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:760760/1‑76 (MQ=255)
aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:552595/1‑76 (MQ=255)
|
CGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCAAA > REL606/3028169‑3028314
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
