Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F1 I1 R1
|
17 |
37.2 |
2333115 |
96.5% |
2251455 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,028,358 |
G→T |
A35E (GCG→GAG) |
ECB_02828 ← |
Polysialic acid transport protein kpsM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,028,358 | 0 | G | T | 100.0%
| 165.8
/ NA
| 44 | A35E (GCG→GAG) | ECB_02828 | Polysialic acid transport protein kpsM |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/25); total (19/25) |
ATCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTG > REL606/3028289‑3028429
|
aTCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCcaca < 1:1120814/76‑1 (MQ=255)
aTCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCcaca < 1:1120831/76‑1 (MQ=255)
cGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACaaa < 1:869209/76‑1 (MQ=255)
gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat > 2:293935/1‑76 (MQ=255)
gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat < 2:346614/76‑1 (MQ=255)
gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat < 2:441654/76‑1 (MQ=255)
gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat > 1:394200/1‑76 (MQ=255)
gCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAatat < 1:526554/76‑1 (MQ=255)
tGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGaa < 2:583020/76‑1 (MQ=255)
tGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGaa > 1:554196/1‑76 (MQ=255)
gTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAAc > 2:436430/1‑76 (MQ=255)
gTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAAc > 2:768852/1‑76 (MQ=255)
gTAACCAAAAATGCCCAACAGTAGCAGCAAATGCGCAGAGGGTTCGCGAATCTCCCACAAATATCCCAGACGGAAc > 1:58001/1‑76 (MQ=255)
tAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACt < 2:262283/76‑1 (MQ=255)
aaaaaTGCCCAACAGTATCAGCAAATGCGCAGAGGGGTCGAGAATCTCCCACAAATANCCCAGACGGAACTTACCa < 1:1105445/76‑1 (MQ=255)
aaaaaTGCCCAACAGTATCAGCAAAGGCNCAGAGGGTTTCAGAATCTGCCACAAATATCCCAGACGGAACTTACCa < 1:108022/76‑1 (MQ=255)
aaaaTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCaa > 2:277056/1‑76 (MQ=255)
aaTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAg < 1:526805/76‑1 (MQ=255)
gCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCgt > 1:1125031/1‑76 (MQ=255)
aCAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCg < 1:189593/76‑1 (MQ=255)
gTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAt < 2:956974/76‑1 (MQ=255)
gTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAt < 2:430169/76‑1 (MQ=255)
gTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAt < 2:430158/76‑1 (MQ=255)
aTCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAttt < 1:442141/76‑1 (MQ=255)
tCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTtc > 2:750442/1‑76 (MQ=255)
tCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTtc > 2:932320/1‑76 (MQ=255)
gCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCg > 1:256131/1‑76 (MQ=255)
cAAATGCGCGGAGGNTTCGAGAACCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCgn < 2:164098/76‑2 (MQ=255)
cAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAANGCGTGTTCGTATTTCTCGt < 1:1082944/76‑1 (MQ=255)
aaaTGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTa < 2:1157160/76‑1 (MQ=255)
gcAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaata < 2:440055/76‑1 (MQ=255)
agagGGTTCGAGAATCTCCCACAAATATCCCAGGCGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaataat > 2:1001965/1‑76 (MQ=255)
agagGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaataat < 2:282904/76‑1 (MQ=255)
agGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGc > 1:952034/1‑76 (MQ=255)
agGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGc > 1:937546/1‑76 (MQ=255)
ttCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCctct < 2:630165/76‑1 (MQ=255)
cGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTAc > 1:588839/1‑76 (MQ=255)
gagaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACg < 1:819295/76‑1 (MQ=255)
gagaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACg < 1:436430/76‑1 (MQ=255)
agaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACgg > 2:187347/1‑76 (MQ=255)
agaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACgg > 1:249216/1‑76 (MQ=255)
aaTCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTg > 2:198652/1‑76 (MQ=255)
aaTCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTg > 2:51533/1‑76 (MQ=255)
aaTCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTg < 1:614079/76‑1 (MQ=255)
|
ATCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTG > REL606/3028289‑3028429
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
