breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 398,676 | T→A | I250N (ATC→AAC) | secF → | protein export protein SecF |
MC JC | 475,285 | Δ2,907 bp | [ybaL]–[ushA] | [ybaL], fsr, [ushA] | |
RA | 648,086 | T→C | E271G (GAG→GGG) | mrdB ← | cell wall shape‑determining protein |
RA | 798,255 | T→C | I341V (ATC→GTC) | ECB_00739 ← | hypothetical protein |
RA | 1,004,243 | A→G | intergenic (‑169/+433) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
RA | 1,415,090 | T→C | H30R (CAC→CGC) | ydaE ← | conserved protein |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,028,358 | G→T | A35E (GCG→GAG) | ECB_02828 ← | Polysialic acid transport protein kpsM |
RA | 3,145,995 | C→T | I44I (ATC→ATT) | rpoD → | RNA polymerase sigma factor |
RA | 3,490,419 | A→T | D2E (GAT→GAA) | glpE ← | thiosulfate sulfurtransferase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 4,162,569 | A→C | I572L (ATC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546984–547700 | 593461 | 45762–46478 | 12 [10] | [10] 11 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593562 | 619842–619116 | 25555–26281 | 11 [10] | [10] 11 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |
* | * | ÷ | REL606 | 2842037–2842086 | 2842254–2842226 | 141–218 | 11 [10] | [10] 11 | [metZ]–[metV] | [metZ],metW,[metV] |