breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 398,676 T→A I250N (ATC→AAC)  secF → protein export protein SecF
MC JC 475,285 Δ2,907 bp [ybaL][ushA] [ybaL], fsr, [ushA]
RA 648,086 T→C E271G (GAG→GGG)  mrdB ← cell wall shape‑determining protein
RA 798,255 T→C I341V (ATC→GTC)  ECB_00739 ← hypothetical protein
RA 1,004,243 A→G intergenic (‑169/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,415,090 T→C H30R (CAC→CGC)  ydaE ← conserved protein
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,028,358 G→T A35E (GCG→GAG)  ECB_02828 ← Polysialic acid transport protein kpsM
RA 3,145,995 C→T I44I (ATC→ATT rpoD → RNA polymerase sigma factor
RA 3,490,419 A→T D2E (GAT→GAA glpE ← thiosulfate sulfurtransferase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,162,569 A→C I572L (ATC→CTC)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546984–547700 593461 45762–46478 12 [10] [10] 11 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593562 619842–619116 25555–26281 11 [10] [10] 11 [fepA]–insA‑8 [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8
* * ÷ REL606 2842037–2842086 2842254–2842226 141–218 11 [10] [10] 11 [metZ]–[metV] [metZ],metW,[metV]