Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A134 F1 I1 R1
|
15 |
220.5 |
13917488 |
95.6% |
13305118 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,343 |
G→A |
A43T (GCA→ACA) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,343 | 0 | G | A | 99.6%
| 951.5
/ ‑5.4
| 224 | A43T (GCA→ACA) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (110/113); total (110/114) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
Only 100 of 236 total aligned reads displayed.
CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTT > REL606/3928270‑3928415
|
cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGcacaca < 2:2482233/76‑1 (MQ=255)
cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGcacaca < 2:1353931/76‑1 (MQ=255)
aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACCCaaa > 2:3740872/1‑76 (MQ=255)
aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaaa < 2:4436388/76‑1 (MQ=255)
aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaaa < 2:5409855/76‑1 (MQ=255)
aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaaa < 2:2370997/76‑1 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag < 1:1121178/76‑1 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag > 2:6226419/1‑76 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag < 1:59620/76‑1 (MQ=255)
atatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAagag > 1:3595093/1‑76 (MQ=255)
atatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAagag > 2:1208639/1‑76 (MQ=255)
atGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCCCACAAagaggg > 1:2754526/1‑74 (MQ=255)
atGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTg < 1:4897469/76‑1 (MQ=255)
tGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCCTCCTGAAGCAGCACACCAAGAgggg > 1:3321952/1‑76 (MQ=255)
tGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTgg < 2:2654583/76‑1 (MQ=255)
ggggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGc < 1:3878329/76‑1 (MQ=255)
gggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCg < 1:1515731/76‑1 (MQ=255)
gggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCg < 2:4290645/76‑1 (MQ=255)
ggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGa < 1:4772815/76‑1 (MQ=255)
gCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGaa < 2:1645461/76‑1 (MQ=255)
cTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAg < 1:4579578/76‑1 (MQ=255)
tGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGa < 2:1108520/76‑1 (MQ=255)
tGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGa > 2:6001729/1‑76 (MQ=255)
ggAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGat < 1:3402613/76‑1 (MQ=255)
aaaaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGatat < 2:5819169/76‑1 (MQ=255)
aaaaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGatat < 1:5675695/76‑1 (MQ=255)
aaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCt < 1:138164/76‑1 (MQ=255)
ccTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCCTCCTGAAGCAGCACACAAAGAGTGGCGGAGATATCttt > 2:458510/1‑76 (MQ=255)
tGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTgg < 2:2757149/76‑1 (MQ=255)
ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTggg > 2:3116325/1‑75 (MQ=255)
ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGt < 1:2149537/76‑1 (MQ=255)
ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGAAATCttttgg > 2:337311/1‑73 (MQ=255)
gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg < 1:1816457/76‑1 (MQ=255)
gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg > 2:1928287/1‑76 (MQ=255)
cTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGa > 1:4514928/1‑76 (MQ=255)
cTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGa > 2:3617458/1‑76 (MQ=255)
cTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGa > 1:5323279/1‑76 (MQ=255)
cGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATg > 2:1630874/1‑76 (MQ=255)
gTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATgg > 1:608742/1‑76 (MQ=255)
tATGCGTAAGCAGGACATTATTTTTGTCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 2:6365291/1‑76 (MQ=255)
tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 1:5180188/1‑76 (MQ=255)
tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc < 2:2933938/76‑1 (MQ=255)
tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 2:2157721/1‑76 (MQ=255)
tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc < 1:2472848/76‑1 (MQ=255)
tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 2:6895905/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 1:2374294/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 1:4327407/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg < 1:2775310/76‑1 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 2:3063351/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg < 2:2782654/76‑1 (MQ=255)
gCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGTGTa < 1:3350881/76‑1 (MQ=255)
gCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTa < 2:5304353/76‑1 (MQ=255)
cGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGGACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTAc > 1:6391227/1‑76 (MQ=255)
tAAGCAGGACATTATTTTTGCCATCCTGAAGGAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg > 1:5805392/1‑76 (MQ=255)
tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg > 1:4665827/1‑76 (MQ=255)
aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg > 2:5001064/1‑76 (MQ=255)
aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg > 2:5939052/1‑76 (MQ=255)
aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg < 2:3982926/76‑1 (MQ=255)
gCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGgag < 1:3865215/76‑1 (MQ=255)
cAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGgaga < 2:1755867/76‑1 (MQ=255)
cAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGgaga > 2:4279936/1‑76 (MQ=255)
aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat > 2:4133138/1‑76 (MQ=255)
aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat < 1:1682468/76‑1 (MQ=255)
aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat < 2:2321603/76‑1 (MQ=255)
ggACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGata > 2:3001188/1‑76 (MQ=255)
aCATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAtt > 2:4472531/1‑76 (MQ=255)
ttattTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCa < 1:6513596/76‑1 (MQ=255)
tattTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAg < 2:3263887/76‑1 (MQ=255)
attTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgg < 2:1909393/76‑1 (MQ=255)
tttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgga < 2:729511/76‑1 (MQ=255)
ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat < 2:3925381/76‑1 (MQ=255)
ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat < 2:4819127/76‑1 (MQ=255)
ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat > 2:240815/1‑76 (MQ=255)
tttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatg < 2:2248392/76‑1 (MQ=255)
tttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatg < 2:2541029/76‑1 (MQ=255)
ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgg > 1:3089018/1‑76 (MQ=255)
ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgg < 1:1764038/76‑1 (MQ=255)
ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgg > 1:6760716/1‑76 (MQ=255)
ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAAATATTGCAggatgg > 1:1453574/1‑76 (MQ=255)
tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 1:1076688/1‑76 (MQ=255)
tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 2:866086/1‑76 (MQ=255)
tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga < 1:1955324/76‑1 (MQ=255)
tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 1:3962204/1‑76 (MQ=255)
gCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat < 1:4038237/76‑1 (MQ=255)
gCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat > 1:4429135/1‑76 (MQ=255)
ccATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGAtt > 1:3263856/1‑76 (MQ=255)
aTCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTg < 2:922675/76‑1 (MQ=255)
aTCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTg < 1:1970198/76‑1 (MQ=255)
tCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTgg > 1:1982755/1‑76 (MQ=255)
ccTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGt < 1:3766308/76‑1 (MQ=255)
gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGgttt > 1:5445837/1‑75 (MQ=255)
gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGGTtc > 2:787175/1‑76 (MQ=255)
aaGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTTGttttc > 2:2832972/1‑74 (MQ=255)
aaGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGttttc > 2:6195799/1‑74 (MQ=255)
agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGGTTTGGTTtcct > 1:4457224/1‑76 (MQ=255)
agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcct < 2:4871708/76‑1 (MQ=255)
agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGGTtcct > 1:1020525/1‑76 (MQ=255)
agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGCAGATATTGCAGGATGGATTTGGTTtcct < 2:3810866/76‑1 (MQ=255)
cagcaCACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcctcc > 1:5039727/1‑76 (MQ=255)
agcaCACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCg > 1:6934215/1‑76 (MQ=255)
|
CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTT > REL606/3928270‑3928415
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
