Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A28 F1 I1 R1
|
15 |
45.6 |
2893554 |
96.7% |
2798066 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,343 |
G→A |
A43T (GCA→ACA) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,343 | 0 | G | A | 100.0%
| 187.2
/ NA
| 50 | A43T (GCA→ACA) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/23); total (27/23) |
CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCC > REL606/3928270‑3928412
|
cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGcacaca > 1:979048/1‑76 (MQ=255)
gAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaa > 2:900213/1‑76 (MQ=255)
gAAAATATGGGGCTGGAAAACCTGGCTCGAAAGGGTAAGCAGGAGATTATTTTTTGCATCCTGCACCAGCACACaa > 1:837364/1‑76 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag > 1:419335/1‑76 (MQ=255)
atatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAagag > 2:598177/1‑76 (MQ=255)
tatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGt < 1:152887/76‑1 (MQ=255)
tatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGt > 2:1072041/1‑76 (MQ=255)
tGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTgg > 1:666051/1‑76 (MQ=255)
gggCTGGAAAACCTGGCTCGTATGCGTAAGCGGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCg < 2:241646/76‑1 (MQ=255)
gCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGaa < 1:478275/76‑1 (MQ=255)
cTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAg < 1:444464/76‑1 (MQ=255)
tGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGa < 2:808230/76‑1 (MQ=255)
tGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGa < 2:1033828/76‑1 (MQ=255)
ggAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGat < 1:223364/76‑1 (MQ=255)
ggAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGat < 1:223369/76‑1 (MQ=255)
aCCTGGCTCGTATGCGTAAGCANGACATTATTTGGGCCNTCCTGAAGCAGCACACAACGGGTGGCGAAAANGTCtt > 1:26411/1‑76 (MQ=255)
ccTGGCTCGTATGCGTAAGCAGGACATTATTTTTGTCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCttt > 2:466286/1‑76 (MQ=255)
cTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTg < 1:994116/76‑1 (MQ=255)
cTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTg < 1:1033058/76‑1 (MQ=255)
gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg > 1:832170/1‑76 (MQ=255)
gCTCGTATGCGTAAGCAGGACATTATTTGTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg < 2:847702/76‑1 (MQ=255)
cGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATg < 2:610861/76‑1 (MQ=255)
cGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATg > 1:582151/1‑76 (MQ=255)
gTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATgg > 2:662450/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTNAAACAGCANACAAACAGTGGCGAAGATANCTTTGGTGATGGCg > 2:1189446/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGNTGATGGCg > 1:1267832/1‑76 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg < 1:776700/76‑1 (MQ=255)
aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 1:775836/1‑76 (MQ=255)
gTAAGCAGGACATTATTTTTGCCNTCCTGNAGCAGCACACAAAGNGTAGCGTAGATATCTTAGGTGATGGCGTACt > 2:192110/1‑76 (MQ=255)
gTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACt < 1:989387/76‑1 (MQ=255)
gTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACt < 1:989383/76‑1 (MQ=255)
tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg > 1:567416/1‑76 (MQ=255)
tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg > 2:1434880/1‑76 (MQ=255)
aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg < 2:1245321/76‑1 (MQ=255)
aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat > 2:816506/1‑76 (MQ=255)
aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat > 2:232242/1‑76 (MQ=255)
aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGNACTGGAGat < 1:35691/76‑1 (MQ=255)
aCATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAtt < 1:1145177/76‑1 (MQ=255)
aCATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAtt > 2:638521/1‑76 (MQ=255)
cATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTg > 2:110751/1‑76 (MQ=255)
ttattTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCa > 1:465372/1‑76 (MQ=255)
tttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatg < 2:893513/76‑1 (MQ=255)
tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 2:1098030/1‑76 (MQ=255)
tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 2:1041274/1‑76 (MQ=255)
tGCCATCCNGTAGCAGCACNCAAAGAGTGGCGAAGATATCTTNGGTGATGGCGTACTGGAGATATTGCAggatgga < 2:1211318/76‑1 (MQ=255)
gCCATCCTGAAGCAGCACACAAAGAGTCGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat > 2:99029/1‑76 (MQ=255)
cATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGAttt < 2:271294/76‑1 (MQ=255)
cATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGAttt > 1:748215/1‑76 (MQ=255)
tGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGttt > 2:1260661/1‑76 (MQ=255)
tGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGttt < 2:1110310/76‑1 (MQ=255)
gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtc > 2:378292/1‑76 (MQ=255)
gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtc < 1:1252058/76‑1 (MQ=255)
gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtc < 1:1141181/76‑1 (MQ=255)
cagcaCACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcctcc > 1:306615/1‑76 (MQ=255)
|
CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCC > REL606/3928270‑3928412
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
