Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A44 F1 I1 R1
|
19 |
54.5 |
3474856 |
97.7% |
3394934 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,165,556 |
C→T |
G96S (GGC→AGC) |
yehU ← |
predicted sensory kinase in two‑component system with YehT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,165,556 | 0 | C | T | 100.0%
| 97.9
/ NA
| 27 | G96S (GGC→AGC) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/11); total (16/11) |
AGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCC‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACG > REL606/2165483‑2165628
|
aGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAgct‑gc > 1:1243165/1‑76 (MQ=255)
tCCTTCAACGATGGTTGAGATNATGCTACTTAGCGCGGCCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTc < 2:283915/76‑1 (MQ=255)
ccTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCa < 2:998344/76‑1 (MQ=255)
aaCGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGacca < 2:743075/76‑1 (MQ=255)
tGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAg < 1:1303510/76‑1 (MQ=255)
tGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAg < 1:41612/76‑1 (MQ=255)
tGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCa > 2:1381423/1‑76 (MQ=255)
tGCAACTTAGCGCGGTCATTCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCgacgac > 1:1238336/1‑76 (MQ=255)
tGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCgacgac > 2:1264769/1‑76 (MQ=255)
cAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACgaccg > 1:907661/1‑76 (MQ=255)
tCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTaa > 2:1014938/1‑76 (MQ=255)
cATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg > 2:1727008/1‑76 (MQ=255)
atcccccccACCGAAAATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgc < 1:1229448/73‑1 (MQ=255)
cccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc > 2:989538/1‑76 (MQ=255)
ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc < 1:1190968/76‑1 (MQ=255)
ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc > 1:1278179/1‑76 (MQ=255)
ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc > 2:1267398/1‑76 (MQ=255)
ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc < 1:1611086/76‑1 (MQ=255)
ccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAt > 2:1443993/1‑76 (MQ=255)
cATCGAATATCGATGTACGCT‑GCCGGTCAGACCAACCAGCCCCCCGACGACCGGACCGCCGAGTAAGCCGCCCAtt < 2:1676108/76‑1 (MQ=255)
tCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc < 1:869943/76‑1 (MQ=255)
cGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc > 1:1387790/1‑76 (MQ=255)
cGAATATCGATG‑AAGCCTGCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc < 1:1158789/76‑1 (MQ=255)
gAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg > 2:990210/1‑76 (MQ=255)
aaTATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgc > 1:670937/1‑76 (MQ=255)
cGATGTAAGCT‑GCCGGTCAGACCAACCAGTCCACCGACGACCGGATCGCCGAGTAAGCCGCCCATTACCGCGCCTa < 2:295283/76‑1 (MQ=255)
gATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTAt > 1:1667823/1‑76 (MQ=255)
tAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa > 2:1478039/1‑76 (MQ=255)
aaGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCAc < 2:444937/76‑1 (MQ=255)
aGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACg > 1:772902/1‑76 (MQ=255)
|
AGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCC‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACG > REL606/2165483‑2165628
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
