Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A32 F1 I1 R1
|
13 |
41.3 |
2638873 |
95.5% |
2520123 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,886,177 |
A→C |
intergenic (‑7/+662) |
yobF ← / ← yebO |
hypothetical protein/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,886,177 | 0 | A | C | 97.4%
| 151.8
/ ‑5.4
| 39 | intergenic (‑7/+662) | yobF/yebO | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (22/16); total (23/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 |
CGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACA > REL606/1886104‑1886246
|
cGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCAc < 2:843187/76‑1 (MQ=255)
agagAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTa > 2:984351/1‑76 (MQ=255)
agagAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTa > 1:559480/1‑76 (MQ=255)
aaGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTc > 2:681955/1‑76 (MQ=255)
aaGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTc > 2:681931/1‑76 (MQ=255)
aGCGGTATTCAACGTCAACGTGGTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCg < 2:40217/76‑1 (MQ=255)
tATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTaa > 1:1174348/1‑76 (MQ=255)
aacgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGa > 2:349469/1‑76 (MQ=255)
tcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAGCACGCAATc > 2:1307675/1‑76 (MQ=255)
tcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATc < 1:51207/76‑1 (MQ=255)
caacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCt < 2:1161853/76‑1 (MQ=255)
caacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCt < 2:41252/76‑1 (MQ=255)
caacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCt > 2:327174/1‑76 (MQ=255)
caacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCt < 1:231129/76‑1 (MQ=255)
gtgtTTACTCAGGACTTCTTTACTGAAGATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAAGCTGTTa > 1:1297722/1‑76 (MQ=255)
gtgtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTa > 2:8570/1‑76 (MQ=255)
tgtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTAt < 1:960123/76‑1 (MQ=255)
tgtTTACTCAGGACNTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTan < 2:178738/76‑2 (MQ=255)
gtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATc > 2:480855/1‑76 (MQ=255)
ttACTCAGGACTTCTTTACTGTAACTGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATcac < 1:1241180/76‑1 (MQ=255)
aCTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACAt > 2:330456/1‑76 (MQ=255)
cAGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACa > 2:830845/1‑76 (MQ=255)
aGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACaa > 2:806110/1‑76 (MQ=255)
aGGACTTCTTTACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACaa < 1:1281528/76‑1 (MQ=255)
tttACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAAtt > 2:741776/1‑76 (MQ=255)
tACTGAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTAt < 1:457338/76‑1 (MQ=255)
gAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGACNTCNGTTATCACATACAANGTTAATTATGGCa > 2:1190258/1‑76 (MQ=255)
gAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCa < 1:1045861/76‑1 (MQ=255)
gAAAATGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCa < 2:468388/76‑1 (MQ=255)
aaaaTGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCaa > 1:551471/1‑76 (MQ=255)
aaaTGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAg > 1:685863/1‑76 (MQ=255)
aaTGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc > 2:367696/1‑76 (MQ=255)
aTGCCACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGcc < 1:327174/76‑1 (MQ=255)
ccACACATAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAtt > 2:943032/1‑76 (MQ=255)
acaTAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTa < 1:147543/76‑1 (MQ=255)
acaTAAACAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGCCAAGCCATTTTTa < 1:84441/76‑1 (MQ=255)
tAAACAGCACTGTACCTAGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAAc < 1:147811/76‑1 (MQ=255)
aaaCAGCACTGTACCTCGTTTAACCCGCAATCTGTTATCACATCNAANGTTAATTATGGCAANCCATTTTTAAACa > 2:1184737/1‑76 (MQ=255)
aaaCAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACa > 2:606590/1‑76 (MQ=255)
aaaCAGCACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACa > 2:1031209/1‑76 (MQ=255)
|
CGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACA > REL606/1886104‑1886246
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
