breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	1,329	G→A	A332T (GCG→ACG)	thrA →	bifunctional aspartokinase I/homeserine dehydrogenase I
JC JC	1,125,562	IS186 (+) +6 bp	coding (131‑136/2544 nt)	mdoH →	glucosyltransferase MdoH
RA	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I
JC	1,733,297	(CGTATGAA)1→2	coding (333/1413 nt)	pykF →	pyruvate kinase
RA	1,886,177	A→C	intergenic (‑7/+662)	yobF ← / ← yebO	hypothetical protein/hypothetical protein
RA	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein
JC	3,512,434	Δ6 bp	intergenic (‑176/‑152)	yhhX ← / → yhhY	predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase
RA	3,762,741	A→T	K662I (AAA→ATA)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA	3,911,972	(T)7→8	intergenic (‑173/‑150)	yifB ← / → ilvL	predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA	4,163,094	G→T	G747C (GGT→TGT)	rpoB →	DNA‑directed RNA polymerase subunit beta
JC JC	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	546991–547699	593379	45681–46389	12 [11]	[11] 12	[insB‑6]–[fepA]	[insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
*	*	÷	REL606	593570	619834–619116	25547–26265	12 [10]	[11] 12	[fepA]–insA‑8	[fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8
*	*	÷	REL606	2032350–2032441	2055696–2055574	23134–23347	14 [11]	[10] 13	[manB]–[cpsG]	[manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,wzx,rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG]