Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A69 F1 I1 R1
|
20 |
59.3 |
3764421 |
95.9% |
3610079 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,089 |
A→T |
E745V (GAA→GTA) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,089 | 0 | A | T | 93.2%
| 272.8
/ 9.7
| 74 | E745V (GAA→GTA) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base T (34/35); total (36/38) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 |
GTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGC > REL606/4163017‑4163160
|
gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGc > 1:861975/1‑76 (MQ=255)
gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGc < 2:1402695/76‑1 (MQ=255)
tggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCa > 2:236908/1‑76 (MQ=255)
ggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACAAGAAGTATCCGGGTGTAGCAg > 1:1119174/1‑76 (MQ=255)
gtgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAgg < 1:1330465/76‑1 (MQ=255)
tgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGt < 1:619240/76‑1 (MQ=255)
tCGTTCAGTACGTGGATGCTTCGCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTAt < 1:121718/76‑1 (MQ=255)
tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTAt < 1:1861625/76‑1 (MQ=255)
tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTAt < 1:670427/76‑1 (MQ=255)
cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATc > 2:805282/1‑76 (MQ=255)
gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCg < 1:912844/76‑1 (MQ=255)
gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCg < 1:986388/76‑1 (MQ=255)
ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGa < 2:21022/76‑1 (MQ=255)
tCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGAc < 1:1505530/76‑1 (MQ=255)
cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACa > 1:564924/1‑76 (MQ=255)
cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACa > 2:102786/1‑76 (MQ=255)
cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACa > 1:1720762/1‑76 (MQ=255)
aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACAt < 2:148106/76‑1 (MQ=255)
aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACAt < 2:867917/76‑1 (MQ=255)
aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACAt < 1:215255/76‑1 (MQ=255)
tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCt < 1:210433/76‑1 (MQ=255)
tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCt < 1:1467242/76‑1 (MQ=255)
aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTa > 1:763998/1‑76 (MQ=255)
aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTa < 2:1184547/76‑1 (MQ=255)
gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGACGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACa > 2:191425/1‑76 (MQ=255)
gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACa > 2:1586510/1‑76 (MQ=255)
gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACa > 1:1525435/1‑76 (MQ=255)
tGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACaa > 1:1661232/1‑76 (MQ=255)
gATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAAcc > 2:879137/1‑76 (MQ=255)
gATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAAcc > 1:1043668/1‑76 (MQ=255)
cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGAc > 2:1667912/1‑76 (MQ=255)
tCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCa > 2:98855/1‑76 (MQ=255)
ccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCaaa < 2:455564/76‑1 (MQ=255)
ccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCAGGTGTAGCAGGTATCGACATCTACAACCTGACCaaa < 2:455548/76‑1 (MQ=255)
cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAAt > 1:104544/1‑76 (MQ=255)
cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAAt < 2:460944/76‑1 (MQ=255)
tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATac > 1:582380/1‑76 (MQ=255)
tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATac > 1:47643/1‑76 (MQ=255)
aTCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATaca < 1:596579/76‑1 (MQ=255)
tCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATacac < 1:706537/76‑1 (MQ=255)
cGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACAcc < 1:399695/76‑1 (MQ=255)
gTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACAccc < 1:677794/76‑1 (MQ=255)
ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 2:222993/1‑76 (MQ=255)
ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 1:1070700/1‑76 (MQ=255)
ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 2:286825/1‑76 (MQ=255)
ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 1:285434/1‑76 (MQ=255)
tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGt > 1:158833/1‑76 (MQ=255)
tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGt > 1:1663799/1‑76 (MQ=255)
aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGtt < 2:1084357/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt < 1:928641/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt < 2:704022/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt < 1:1213603/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt > 2:1403975/1‑76 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt > 1:562292/1‑76 (MQ=255)
aaaGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTa > 1:1872712/1‑76 (MQ=255)
aaGTTAACGAAGACGATATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTaa < 1:48076/76‑1 (MQ=255)
aaGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAACTACACCCGTTCTaa > 1:1655636/1‑76 (MQ=255)
gTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc < 1:57266/76‑1 (MQ=255)
ttAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa > 1:714743/1‑76 (MQ=255)
aCGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGaa > 1:1629447/1‑76 (MQ=255)
gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca < 1:1175022/76‑1 (MQ=255)
aaGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGATCTAACCAGAacac > 1:773460/1‑76 (MQ=255)
aGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACAcc > 2:745024/1‑76 (MQ=255)
aCGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg < 2:493421/76‑1 (MQ=255)
aCGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg < 2:1019946/76‑1 (MQ=255)
gagaTGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTa < 1:131566/76‑1 (MQ=255)
agaTGTAGCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTAt < 1:16965/76‑1 (MQ=255)
gaTGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATc < 1:1724968/76‑1 (MQ=255)
tGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa < 2:707725/76‑1 (MQ=255)
tGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa > 1:1031712/1‑76 (MQ=255)
aTCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa > 1:911562/1‑76 (MQ=255)
aTCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa > 2:465052/1‑76 (MQ=255)
cGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGAt < 1:396641/76‑1 (MQ=255)
gggTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATg < 2:762804/76‑1 (MQ=255)
gggTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATg < 2:303953/76‑1 (MQ=255)
ggTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGc > 1:222645/1‑76 (MQ=255)
|
GTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGC > REL606/4163017‑4163160
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
