breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 473,924 A→C V461G (GTC→GGC)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 795,321 (G)6→7 intergenic (+26/+16) ECB_00736 → / ← ECB_00737 hypothetical protein/hypothetical protein
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
JC JC 1,941,961 IS186 (–) +8 bp :: Δ2 bp coding (1853‑1860/2079 nt) flhA ← flagellar biosynthesis protein A
MC JC 2,100,308 Δ22,146 bp ogrK[ECB_02013] 27 genes
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,562,715 G→A R317C (CGT→TGT)  yfgA ← hypothetical protein
JC JC 3,015,771 IS150 (–) +3 bp coding (1192‑1194/1677 nt) ECB_02816 → KpsD protein
JC 3,339,054 Δ5 bp coding (884‑888/966 nt) yhdG → tRNA‑dihydrouridine synthase B
RA 3,488,896 T→G E104A (GAA→GCA)  glpR ← DNA‑binding transcriptional repressor
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,972 (T)7→6 intergenic (‑173/‑150) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 4,163,089 A→T E745V (GAA→GTA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,164,845 T→G I1330M (ATT→ATG rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,262,921 C→T M305I (ATG→ATA actP ← acetate permease
RA 4,275,544 T→C D135G (GAT→GGT)  yjcO ← hypothetical protein
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546984–547700 593405 45706–46422 19 [18] [18] 19 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593573 619850–619116 25544–26278 20 [17] [18] 21 [fepA]–insA‑8 [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8