breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,492,0820TG45.8% 63.0 / 21.8 48V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6064,393,0610TG37.5% 47.8 / 12.6 32V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6061,004,1060AC36.0% 97.8 / 12.3 50intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL606273,5810TG35.2% 99.0 / 10.4 54G136G (GGT→GGGyagUconserved inner membrane protein
*REL6061,484,1050AG30.2% 65.6 / 22.5 43G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6062,296,3290AC28.8% 139.2 / 15.8 59intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6061,416,8380TG27.7% 171.5 / 21.9 83intergenic (+1/‑4)ECB_01334/ECB_01335putative Lysozyme/conserved hypothetical protein

Marginal new junction evidence (lowest skew 10 of 72 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3201814 =66 (1.120)21 (0.380) 11/142 3.9 18.6% coding (366/1104 nt) yhaC hypothetical protein
?REL606 3202251 = 121 (2.180)coding (803/1104 nt) yhaC hypothetical protein
* ? REL606 = 204227484 (1.430)15 (0.290) 8/134 4.6 21.1% coding (547/1428 nt) wzx O antigen translocase (Wzx)
?REL606 = 2042743 37 (0.710)coding (78/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2041054 =71 (1.210)13 (0.240) 8/136 4.7 15.9% coding (330/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041468 = 73 (1.370)coding (1353/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2042091 =53 (0.900)8 (0.150) 7/140 5.2 10.7% coding (730/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2042518 = 84 (1.530)coding (303/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2038432 =65 (1.110)16 (0.310) 6/134 5.4 21.6% coding (517/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 2038941 = 58 (1.110)coding (8/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 3022392 =58 (0.990)11 (0.210) 6/136 5.5 13.5% coding (293/1140 nt) ECB_02821 hypothetical protein
?REL606 3022865 = 88 (1.660)intergenic (‑181/+393) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
* ? REL606 2036832 =54 (0.920)7 (0.130) 6/136 5.5 12.4% coding (127/1113 nt) wbbC putative glycosyltransferase WbbC
?REL606 2037060 = 50 (0.940)coding (1071/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 1219479 =65 (1.110)13 (0.240) 6/136 5.5 15.6% intergenic (+117/‑283) ymgF/ycgH hypothetical protein/predicted protein; b4491
?REL606 1220055 = 82 (1.540)pseudogene (294/2622 nt) ycgH predicted protein; b4491
* ? REL606 3022508 =59 (1.010)10 (0.200) 5/126 5.6 14.0% coding (177/1140 nt) ECB_02821 hypothetical protein
?REL606 3023074 = 73 (1.480)intergenic (‑390/+184) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
* ? REL606 = 373726187 (1.480)6 (0.110) 6/142 5.7 6.7% coding (566/1254 nt) waaL Lipid A‑core, surface polymer ligase
?REL606 = 3737695 85 (1.530)coding (132/1254 nt) waaL Lipid A‑core, surface polymer ligase