Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A20 F1 I1 R1
|
17 |
44.7 |
2849266 |
96.8% |
2758089 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,762,741 |
A→T |
K662I (AAA→ATA) |
spoT → |
bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,762,741 | 0 | A | T | 100.0%
| 211.5
/ NA
| 54 | K662I (AAA→ATA) | spoT | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (27/27); total (27/27) |
GTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATGCG > REL606/3762669‑3762813
|
gTGCGCTGTCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa < 1:1148358/76‑1 (MQ=255)
gTGCGCTGTCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa < 1:1148373/76‑1 (MQ=255)
gcgcTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATg < 2:1416363/76‑1 (MQ=255)
tGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTcg > 2:1106634/1‑76 (MQ=255)
ggCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc < 1:364993/76‑1 (MQ=255)
gCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg > 1:1108758/1‑76 (MQ=255)
gCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAAGACGGAAGAGATAGATGGTcgcg < 2:1082123/76‑1 (MQ=255)
aaaCCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc > 1:572987/1‑76 (MQ=255)
aaaCCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc > 1:370093/1‑76 (MQ=255)
aCGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg > 1:1040529/1‑76 (MQ=255)
cAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAt < 2:115019/76‑1 (MQ=255)
aaTTAACACCACGACTTCGAATATTCAAAGTTTNAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt > 2:1205968/1‑76 (MQ=255)
aaTTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt < 1:1198673/76‑1 (MQ=255)
aTTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc < 2:658298/76‑1 (MQ=255)
ttAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCg < 2:105248/76‑1 (MQ=255)
tAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGt > 2:426568/1‑76 (MQ=255)
naaCACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACNGCGCCTTTATTCGt > 2:198456/2‑76 (MQ=255)
acacCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGAGGGTCGCGTCTACAGCGCCTTTATTCGTCt < 2:582651/76‑1 (MQ=255)
caccacGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTg > 2:967915/1‑76 (MQ=255)
caccacGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTg < 1:1189796/76‑1 (MQ=255)
ccacGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGAc < 2:816843/76‑1 (MQ=255)
acGACTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCg < 1:279154/76‑1 (MQ=255)
cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCg > 2:717111/1‑76 (MQ=255)
tCGAATATTCAAAGTTTGAAGACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCCGACCGCTCGTg < 1:288156/76‑1 (MQ=255)
cGAATATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGa < 2:1421360/76‑1 (MQ=255)
aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGAcc > 1:696326/1‑76 (MQ=255)
atTCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCgtg < 2:798102/76‑1 (MQ=255)
ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCgtgt < 2:170/76‑1 (MQ=255)
tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGNGCCTTTATTCGTCTGACCGCTCGTGACCGtgtg < 2:1199391/76‑1 (MQ=255)
aaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTANAGCGCCTGTACTCGTGTGACCGCTCGTGACCGTGNGCAt > 1:162884/1‑76 (MQ=255)
aaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGACTGACCGCTCNTGACCGTGTGCaa > 1:185798/1‑75 (MQ=255)
aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATc > 2:399665/1‑76 (MQ=255)
gTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCt < 2:109202/76‑1 (MQ=255)
tttGAATTCGGAAGAGATAGCTNNTCGCGTCTACNNGGCCTTTAGTCGTCGGACCGCTCGTGACCGTGTGCATCTg < 1:1272545/76‑1 (MQ=255)
tttGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTg < 1:711051/76‑1 (MQ=255)
ttGAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTgg > 1:983813/1‑76 (MQ=255)
gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCg > 1:914723/1‑76 (MQ=255)
gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCg < 1:684658/76‑1 (MQ=255)
gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCg < 1:878317/76‑1 (MQ=255)
aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGa > 2:1318799/1‑76 (MQ=255)
aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGa > 1:653357/1‑76 (MQ=255)
aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGa > 2:630766/1‑76 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAat > 2:1052024/1‑76 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAat > 2:1241057/1‑76 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAat > 2:848643/1‑76 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAat > 2:1153500/1‑76 (MQ=255)
aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAata > 2:124000/1‑76 (MQ=255)
cGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAatat < 1:1044809/76‑1 (MQ=255)
cGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAatat < 1:1179950/76‑1 (MQ=255)
cGGAAGAGATAGATGGTCGCGTCTACAGCGCCTNTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAatat > 2:1200867/1‑76 (MQ=255)
ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATc < 2:1077792/76‑1 (MQ=255)
ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATc < 2:325319/76‑1 (MQ=255)
ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATc > 1:1026819/1‑76 (MQ=255)
gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCa < 2:60115/76‑1 (MQ=255)
agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATg > 1:750041/1‑76 (MQ=255)
agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATgcg > 2:372805/1‑76 (MQ=255)
|
GTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATGCG > REL606/3762669‑3762813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
