Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F1 I1 R1
|
7 |
175.1 |
22577466 |
95.2% |
21493747 |
37.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,762,741 |
A→T |
K662I (AAA→ATA) |
spoT → |
bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,762,741 | 0 | A | T | 100.0%
| 447.5
/ NA
| 124 | K662I (AAA→ATA) | spoT | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (78/46); total (78/46) |
Only 100 of 133 total aligned reads displayed.
ACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCT > REL606/3762707‑3762774
|
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg > 2:1281721/1‑37 (MQ=255)
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg > 2:3552365/1‑37 (MQ=255)
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg > 2:9719058/1‑37 (MQ=255)
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg > 2:8825398/1‑37 (MQ=255)
cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa > 1:7427565/1‑37 (MQ=255)
cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa < 2:8078721/37‑1 (MQ=255)
cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa < 1:7222499/37‑1 (MQ=255)
ttCGAATATTCAAAGTTTGAATACGGAAGagatagat > 2:8715472/1‑37 (MQ=255)
ttCGAATATTCAAAGTTTGAATACGGAAGagatagat > 1:3240353/1‑37 (MQ=255)
ttCGAATATTCAAAGTTTGAATACGGAAGagatagat < 1:10280387/37‑1 (MQ=255)
ttCGAATATTCAAAGTTTGAATACGGAAGagatagat > 2:7270509/1‑37 (MQ=255)
tCGAATATTCAAAGTTTGAATACGGAAGAGATAGATg > 1:5947909/1‑37 (MQ=255)
tCGAATATTCAAAGTTTGAATACGGAAGAGATAGATg > 1:8675952/1‑37 (MQ=255)
cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg < 2:811415/37‑1 (MQ=255)
cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg > 2:5418283/1‑37 (MQ=255)
cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg < 2:8609225/37‑1 (MQ=255)
cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg > 1:4128152/1‑37 (MQ=255)
gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt > 1:2200022/1‑37 (MQ=255)
gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt < 2:8571716/37‑1 (MQ=255)
gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt < 1:3980088/37‑1 (MQ=255)
gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt > 2:8891453/1‑37 (MQ=255)
gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt > 2:6578214/1‑37 (MQ=255)
aaTATTCAAAGTTTTAATACGGAAGAGATAGATGGTc < 2:7086882/37‑1 (MQ=37)
aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTc < 2:482688/37‑1 (MQ=255)
aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTc > 1:9553305/1‑37 (MQ=255)
aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTc < 1:157033/37‑1 (MQ=255)
tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc > 1:10641232/1‑37 (MQ=255)
tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc > 2:2754126/1‑37 (MQ=255)
tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc < 2:7987599/37‑1 (MQ=255)
tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc < 2:6558970/37‑1 (MQ=255)
atTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg > 2:10825613/1‑37 (MQ=255)
atTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg > 1:11252403/1‑37 (MQ=255)
atTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg > 1:8106456/1‑37 (MQ=255)
ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt < 2:11068011/37‑1 (MQ=255)
ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt < 2:8836089/37‑1 (MQ=255)
ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt < 1:6391853/37‑1 (MQ=255)
ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt > 1:11169378/1‑37 (MQ=255)
tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc < 2:1573176/37‑1 (MQ=255)
tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc > 1:8979074/1‑37 (MQ=255)
tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc > 1:4617961/1‑37 (MQ=255)
cAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCt > 2:1291698/1‑37 (MQ=255)
aaaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTa < 2:468902/37‑1 (MQ=255)
aaaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTa < 1:4382037/37‑1 (MQ=255)
aaaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTa > 1:9289147/1‑37 (MQ=255)
aaaGTTTGAATACGGAAGAGATAGAGGGTCGCGTCTa < 2:6104706/37‑1 (MQ=37)
aaaGTNTGAATACGGAAGAGATAGATGGTCGCGTCTa > 2:10601390/1‑37 (MQ=38)
aaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTAc > 1:2633217/1‑37 (MQ=255)
aaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTAc > 1:7151794/1‑37 (MQ=255)
aaGTTTGAATACGGAAGAGATAGATGGTCGCGTATAc > 1:1485521/1‑37 (MQ=37)
aGTTTGCATCCGGAAGAGATAGATGGTCGCGTCTACa < 1:1935043/37‑1 (MQ=25)
aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACa < 1:11160321/37‑1 (MQ=255)
aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACa < 2:5269474/37‑1 (MQ=255)
aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACa > 2:4087698/1‑37 (MQ=255)
gTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAg > 1:6006012/1‑37 (MQ=255)
gTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAg > 2:3999915/1‑37 (MQ=255)
tttGAATACGGAAGAGATAGATGGTCGCGTCTACAgc < 2:5526039/37‑1 (MQ=255)
tttGAATACGGAAGAGATAGATGGTCGCGTCTACAgc > 2:9098768/1‑37 (MQ=255)
tttGAATACGGAAGAGATAGATGGTCGCGTCTACAgc < 2:1522098/37‑1 (MQ=255)
ttGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg > 2:4504845/1‑37 (MQ=255)
ttGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg > 1:886265/1‑37 (MQ=255)
ttGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg > 1:4941727/1‑37 (MQ=255)
tGAATACGGAAGAGATAGATGGTCGCGTCTACAgcgc < 2:7196530/37‑1 (MQ=255)
gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGcc < 1:923629/37‑1 (MQ=255)
gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGcc > 1:5131009/1‑37 (MQ=255)
aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCt > 2:1718069/1‑37 (MQ=255)
aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCt > 2:6693646/1‑37 (MQ=255)
aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCt > 2:1453505/1‑37 (MQ=255)
aTACGGAAGAGATAGATTGTCGCGTCTACAGCGCCtt < 1:8841280/37‑1 (MQ=37)
aTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCtt > 1:2004797/1‑37 (MQ=255)
aTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCtt < 2:10730402/37‑1 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt > 1:1720800/1‑37 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt < 2:11109846/37‑1 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt > 2:4118809/1‑37 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt > 1:10996051/1‑37 (MQ=255)
tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt > 1:5856348/1‑37 (MQ=255)
aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCtttc > 1:3043561/1‑36 (MQ=38)
aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTa < 2:7835761/37‑1 (MQ=255)
aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTa > 1:5026920/1‑37 (MQ=255)
aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTa > 1:6454680/1‑37 (MQ=255)
cGGAAGGGATAGATGGTCGCGTCTACAGCGCCTTTAt < 1:7187405/37‑1 (MQ=38)
cGGAAGAGATAGGGGGTCGCGTCTACAGCGCCTTTAt < 1:1419480/37‑1 (MQ=37)
cGGAAGAGATAGATGGTCGCGTCTACAGCGCCGTTAt > 1:954427/1‑37 (MQ=38)
ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt > 1:9820470/1‑37 (MQ=255)
ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt > 1:6480295/1‑37 (MQ=255)
ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt > 1:7824961/1‑37 (MQ=255)
gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc > 2:9858601/1‑37 (MQ=255)
gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc > 1:9873076/1‑37 (MQ=255)
gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc < 1:4473146/37‑1 (MQ=255)
gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc > 1:2764566/1‑37 (MQ=255)
gAAGAGATAGATGGTCGCGTCTACAGCGACTTTATTc > 2:6050411/1‑37 (MQ=37)
aaGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCg > 2:808623/1‑37 (MQ=255)
aaGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCg < 2:1854107/37‑1 (MQ=255)
agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt > 2:5046961/1‑37 (MQ=255)
agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt > 2:10841508/1‑37 (MQ=255)
agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt > 1:9493737/1‑37 (MQ=255)
agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt < 1:3057188/37‑1 (MQ=255)
gagaTAGATGGTCGCGTCTACAGCGCCTTTATTCGTc < 2:7359728/37‑1 (MQ=255)
agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCt > 2:5611678/1‑37 (MQ=255)
agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCt < 1:3999450/37‑1 (MQ=255)
agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCt < 1:10926553/37‑1 (MQ=255)
|
ACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCT > REL606/3762707‑3762774
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A