Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A20 F1 I1 R1
|
17 |
44.7 |
2849266 |
96.8% |
2758089 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,260 |
T→A |
I15N (ATC→AAC) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,260 | 0 | T | A | 98.0%
| 192.0
/ NA
| 52 | I15N (ATC→AAC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (21/29); minor base G (0/1); total (22/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCT > REL606/3928187‑3928332
|
tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACa > 2:567639/1‑76 (MQ=255)
ttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAacac > 1:41742/1‑76 (MQ=255)
aaGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGc < 1:277349/76‑1 (MQ=255)
aaGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTACTGAGCTGAACACTCTCGGc < 1:9554/76‑1 (MQ=255)
aGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCg < 2:775747/76‑1 (MQ=255)
tttAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaa > 1:810689/1‑76 (MQ=255)
tttAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaa > 2:85496/1‑76 (MQ=255)
tAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaaaa > 2:1036376/1‑76 (MQ=255)
tAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaaaa > 1:340090/1‑76 (MQ=255)
tAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaaaa < 1:220437/76‑1 (MQ=255)
aaGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAat < 1:319563/76‑1 (MQ=255)
cacacaACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGc > 2:380726/1‑76 (MQ=255)
cacaCCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGc > 2:1319128/1‑76 (MQ=255)
acacCACTATGAATCTTACCGAATTAAAGAATNCGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGNGCt > 2:1333231/1‑76 (MQ=255)
acacCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCt > 2:1031037/1‑76 (MQ=255)
acacCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCt > 1:819231/1‑76 (MQ=255)
ccacTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGNGCTGAACACTCTCGGCGAAAATATGGGGCTGGa < 2:1211749/76‑1 (MQ=255)
cacTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGaa < 2:1123517/76‑1 (MQ=255)
acTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGaaa < 1:1392110/76‑1 (MQ=255)
acTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGaaa < 1:755578/76‑1 (MQ=255)
gAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTg < 2:13435/76‑1 (MQ=255)
tCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt < 2:915497/76‑1 (MQ=255)
cTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGcac > 2:39335/1‑74 (MQ=255)
cTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTc < 1:1204231/76‑1 (MQ=255)
tACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTAGGTCGt < 1:85496/76‑1 (MQ=255)
nccGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGNAAACCTGGCTCGTa > 2:203603/2‑76 (MQ=255)
ccGAATTAAAGAGTACGCCGGTTTCTGAGCTGNACACTCTCNGCGAAAATATGGGGCTGGGNAACCTGGCTCGtct > 2:1170447/1‑74 (MQ=255)
aaTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCg < 2:19255/76‑1 (MQ=255)
aaTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCg < 2:844628/76‑1 (MQ=255)
aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGNAAACCTGGCTCGTATGCGt < 1:1325760/76‑1 (MQ=255)
aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt > 1:1064933/1‑76 (MQ=255)
aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt < 1:512438/76‑1 (MQ=255)
aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt < 2:903946/76‑1 (MQ=255)
ttAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa < 2:1369518/76‑1 (MQ=255)
ttAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa > 2:299323/1‑76 (MQ=255)
tAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGCAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa < 1:134904/76‑1 (MQ=255)
aaaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAg < 1:369248/76‑1 (MQ=255)
aaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc < 1:756293/76‑1 (MQ=255)
aaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc < 2:326897/76‑1 (MQ=255)
aaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTANGCGTAAGc > 1:1252865/1‑76 (MQ=255)
aCGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACa < 1:252363/76‑1 (MQ=255)
cGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACat < 2:562647/76‑1 (MQ=255)
ccGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACatta < 1:1202049/76‑1 (MQ=255)
ccGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACatta > 1:203567/1‑76 (MQ=255)
cGGTTTCTGAGCTGAGCACTCTGGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACattat < 2:46816/76‑1 (MQ=255)
cGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACattat < 1:867162/76‑1 (MQ=255)
cGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACattat < 1:1229162/76‑1 (MQ=255)
tCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTg > 2:1020797/1‑76 (MQ=255)
cTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGc < 2:799577/76‑1 (MQ=255)
tGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGcc > 1:348926/1‑76 (MQ=255)
gAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCa > 2:256350/1‑76 (MQ=255)
gAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCa > 1:838203/1‑76 (MQ=255)
aGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCAt < 1:1231483/76‑1 (MQ=255)
tGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCt > 2:359899/1‑76 (MQ=255)
tGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCt > 2:1023071/1‑76 (MQ=255)
|
TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCT > REL606/3928187‑3928332
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
