Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A46 F1 I1 R1
|
17 |
71.0 |
4433184 |
98.0% |
4344520 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,015,178 |
A→T |
D200V (GAT→GTT) |
ECB_02816 → |
KpsD protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,015,178 | 0 | A | T | 98.7%
| 304.7
/ ‑3.3
| 79 | D200V (GAT→GTT) | ECB_02816 | KpsD protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (40/38); total (40/39) |
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 |
TAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGTA > REL606/3015107‑3015250
|
tAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCa < 1:1628460/76‑1 (MQ=255)
aaTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCag < 1:1109554/76‑1 (MQ=255)
aTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCaga > 1:1335835/1‑76 (MQ=255)
ccTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAgc < 1:1058572/76‑1 (MQ=255)
cTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAgcg < 2:954865/76‑1 (MQ=255)
tGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAgcgc > 2:2000849/1‑76 (MQ=255)
ggTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGcgcg < 2:1588741/76‑1 (MQ=255)
ggTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGcgcg < 2:801526/76‑1 (MQ=255)
ggTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGcgcg > 2:709907/1‑76 (MQ=255)
tCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGa < 1:1260438/76‑1 (MQ=255)
cTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGaa > 1:1127057/1‑76 (MQ=255)
aTGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTa < 1:132452/76‑1 (MQ=255)
gCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGt < 1:1644194/76‑1 (MQ=255)
gtgtGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGa > 1:1626542/1‑76 (MQ=255)
tgtgACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGat > 2:653662/1‑76 (MQ=255)
aCGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATtg > 1:472742/1‑76 (MQ=255)
gTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATtgtg < 1:1166938/76‑1 (MQ=255)
gTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATtgtg > 1:2139845/1‑76 (MQ=255)
tCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATtgtag > 2:1318079/1‑74 (MQ=255)
cTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGt > 2:1613659/1‑76 (MQ=255)
gATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCa < 1:986265/76‑1 (MQ=255)
aTTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCaa < 1:1824133/76‑1 (MQ=255)
aTTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCaa > 1:637831/1‑76 (MQ=255)
aTTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCaa > 1:245554/1‑76 (MQ=255)
ttCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAg > 1:1273303/1‑76 (MQ=255)
tCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgc < 1:1893808/76‑1 (MQ=255)
cGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgcg > 1:1495718/1‑76 (MQ=255)
cGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgcg < 1:3276/76‑1 (MQ=255)
cGTCACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgcg > 2:798680/1‑76 (MQ=255)
ttACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGcgcg > 1:1955751/1‑76 (MQ=255)
tACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCgg > 1:298456/1‑76 (MQ=255)
aCTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGt > 2:1570246/1‑76 (MQ=255)
aCTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGt > 1:1545549/1‑76 (MQ=255)
aCTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGt > 2:1325983/1‑76 (MQ=255)
aCTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGt > 2:2169370/1‑76 (MQ=255)
cTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTa < 1:841756/76‑1 (MQ=255)
cTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTa < 2:2191146/76‑1 (MQ=255)
cAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAAc < 1:122773/76‑1 (MQ=255)
cAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAAc < 1:736427/76‑1 (MQ=255)
cAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAAc < 1:2089954/76‑1 (MQ=255)
aTTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcg > 1:448701/1‑76 (MQ=255)
aTTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGGGGTCAAGCGCGGTACCcg > 2:1090550/1‑76 (MQ=255)
ttATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgc < 2:1785686/76‑1 (MQ=255)
tATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgcg < 1:482014/76‑1 (MQ=255)
tATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgcg < 1:445485/76‑1 (MQ=255)
tATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgcg > 2:1545121/1‑76 (MQ=255)
tATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgcg < 2:2205528/76‑1 (MQ=255)
aTCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgcgg > 2:30309/1‑75 (MQ=255)
aTCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCACGt > 1:828687/1‑76 (MQ=255)
aTCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGGAACcgggc > 1:510150/1‑73 (MQ=255)
tCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTg > 1:931442/1‑76 (MQ=255)
tCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTg < 2:1761334/76‑1 (MQ=255)
tCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGGAACCGCGTg > 2:1576369/1‑76 (MQ=255)
tCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTg < 1:31313/76‑1 (MQ=255)
cTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTgc > 2:88606/1‑76 (MQ=255)
cTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTgc < 2:1520478/76‑1 (MQ=255)
cTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTgc < 2:1452782/76‑1 (MQ=255)
tGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTgcg > 1:990474/1‑76 (MQ=255)
gATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGCTACGTTGATATTGTGGGCAAGCGCGGTAACCGCGTgcgc > 1:1471337/1‑76 (MQ=255)
tCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTc < 1:2076498/76‑1 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa > 2:2066734/1‑76 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa < 1:1082103/76‑1 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa < 2:1483558/76‑1 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa > 1:156795/1‑76 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa < 1:1686367/76‑1 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa < 1:1686373/76‑1 (MQ=255)
aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa > 1:37097/1‑76 (MQ=255)
ggCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAAc < 1:2105467/76‑1 (MQ=255)
cTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGt < 2:1532593/76‑1 (MQ=255)
tGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTc < 2:1417054/76‑1 (MQ=255)
tGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTc > 1:1358829/1‑76 (MQ=255)
ggcggcGTTGTTCCAGAGCGCGGAAGTTATGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCa < 2:371602/76‑1 (MQ=255)
ggcggcGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCa < 2:2174832/76‑1 (MQ=255)
cggcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAAc < 1:514696/76‑1 (MQ=255)
cggcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAAc > 2:688016/1‑76 (MQ=255)
cggcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAAc > 1:899702/1‑76 (MQ=255)
ggcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAAcc < 2:505709/76‑1 (MQ=255)
gcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCt > 1:780566/1‑76 (MQ=255)
gttgttCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGt > 1:1108433/1‑76 (MQ=255)
ttGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGTa > 1:684907/1‑76 (MQ=255)
|
TAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGTA > REL606/3015107‑3015250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
