breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 649,601 | T→C | I401V (ATC→GTC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
JC | 2,103,915 | (CCAG)7→6 | coding (182‑185/216 nt) | ECB_01992 → | hypothetical protein |
RA | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,015,178 | A→T | D200V (GAT→GTT) | ECB_02816 → | KpsD protein |
RA | 3,147,777 | G→T | intergenic (+72/+7) | rpoD → / ← ygjF | RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase |
RA | 3,339,445 | A→G | Y95C (TAC→TGC) | fis → | DNA‑binding protein Fis |
RA | 3,367,985 | C→T | R317H (CGC→CAC) | rpoA ← | DNA‑directed RNA polymerase subunit alpha |
RA | 3,497,333 | T→G | S235R (AGT→CGT) | glgP ← | glycogen phosphorylase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
JC | 3,912,149 | Δ15 bp | coding (28‑42/99 nt) | ilvL → | ilvG operon leader peptide |
RA | 4,163,089 | A→C | E745A (GAA→GCA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546982–547700 | 593499 | 45800–46518 | 27 [25] | [25] 27 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593551 | 619856–619116 | 25566–26306 | 27 [26] | [26] 28 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |