Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A92 F1 I1 R1
|
15 |
58.2 |
3997918 |
96.1% |
3841999 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,162,569 |
A→C |
I572L (ATC→CTC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,162,569 | 0 | A | C | 100.0%
| 279.8
/ NA
| 67 | I572L (ATC→CTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (38/29); total (38/29) |
GAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162498‑4162641
|
gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCCGAAGGTCCGAACATCCGTCTGCTCaa > 2:1384837/1‑76 (MQ=255)
agaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAAc > 2:1199253/1‑76 (MQ=255)
aCGTACAACCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActc > 1:1276918/1‑76 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActct < 2:294330/76‑1 (MQ=255)
gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActctc > 1:1232863/1‑76 (MQ=255)
tccacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActctct < 1:1926232/74‑1 (MQ=255)
acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTg > 2:1677512/1‑76 (MQ=255)
cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGt > 2:1582808/1‑76 (MQ=255)
cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGt < 1:1783902/76‑1 (MQ=255)
cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTcc < 1:1880539/76‑1 (MQ=255)
cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCgt > 2:644616/1‑76 (MQ=255)
gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCgtg > 2:1430904/1‑76 (MQ=255)
cACTACTGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACg < 2:1363401/76‑1 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACg < 1:341809/76‑1 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACg < 2:1104419/76‑1 (MQ=255)
aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGc > 1:1728629/1‑76 (MQ=255)
cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGca < 2:390736/76‑1 (MQ=255)
cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGca < 2:110938/76‑1 (MQ=255)
tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGcac > 2:1848719/1‑76 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa < 1:533548/76‑1 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa > 1:1058645/1‑76 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa > 2:420115/1‑76 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa > 2:70127/1‑76 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa > 2:754874/1‑76 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa < 1:1767857/76‑1 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGa < 1:494347/76‑1 (MQ=255)
gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGAc < 2:1871835/76‑1 (MQ=255)
cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTa > 1:945909/1‑76 (MQ=255)
gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTaa < 2:118818/76‑1 (MQ=255)
ngTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAAc > 1:1512721/2‑76 (MQ=255)
cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCTGTGTACGCACAGACTAAc < 1:572797/76‑1 (MQ=255)
cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAAc > 2:864124/1‑76 (MQ=255)
aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 2:675863/1‑76 (MQ=255)
aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 2:186164/1‑76 (MQ=255)
tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCGCAGACTAACGAAt < 1:1306454/76‑1 (MQ=255)
tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 2:1674685/76‑1 (MQ=255)
ggccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 2:49819/74‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 2:1767214/1‑76 (MQ=255)
ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:909899/1‑76 (MQ=255)
cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 2:366094/1‑76 (MQ=255)
cGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 2:998098/1‑76 (MQ=255)
cGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 2:207189/1‑76 (MQ=255)
naaaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc > 1:1708676/2‑76 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 1:1858373/76‑1 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 2:1230983/1‑76 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 1:1484334/1‑76 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg < 1:1741127/76‑1 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg > 2:931426/1‑76 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg < 2:1831689/76‑1 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg > 1:1884291/1‑76 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:1044650/76‑1 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 2:1119920/76‑1 (MQ=255)
cTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc > 2:1325333/1‑76 (MQ=255)
gAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 1:577332/1‑76 (MQ=255)
gAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 1:1855281/1‑76 (MQ=255)
aaGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 1:1105982/1‑76 (MQ=255)
ggTCCGAACATCGGTCTGCTAAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 2:1318873/76‑1 (MQ=255)
aTCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 2:1963162/76‑1 (MQ=255)
aTCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg > 2:1884466/1‑76 (MQ=255)
aTCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 1:1285172/76‑1 (MQ=255)
tCGGTCTGCTCAACNCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGt > 1:1079971/1‑76 (MQ=255)
cGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg < 1:763038/76‑1 (MQ=255)
ggTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa < 1:884410/76‑1 (MQ=255)
ggTCTGCTCAACGCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa < 2:248929/76‑1 (MQ=255)
tCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc < 1:347060/76‑1 (MQ=255)
tCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc > 1:1832836/1‑76 (MQ=255)
cTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTCCGTATCGTAAAGAGACCg > 2:691842/1‑76 (MQ=255)
cTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg > 2:1429995/1‑76 (MQ=255)
|
GAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162498‑4162641
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
