breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 382,261 A→G I639T (ATT→ACT)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 1,004,196 T→A intergenic (‑122/+480) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
RA 1,290,813 C→T Q260* (CAG→TAG)  hnr → response regulator of RpoS
RA 1,304,966 C→T R425Q (CGG→CAG)  cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,228,283 G→T P96Q (CCG→CAG)  yraL ← predicted methyltransferase
RA 3,499,829 G→T F325L (TTC→TTA)  glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD–[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,162,569 A→C I572L (ATC→CTC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,438,531 T→G D110A (GAC→GCC)  fbp ← fructose‑1,6‑bisphosphatase
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546990–547700 573919 26220–26930 16 [15] [15] 16 [insB‑6]–ECB_00530 [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530
* * ÷ REL606 573958 593387 19430 16 [14] [15] 16 cusS–[fepA] cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593577 619822–619116 25540–26246 16 [15] [13] 16 [fepA]–[insA‑8] [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2774196NA (NA)45 (0.750) 30/144 1.0 51.1% noncoding (1343/1343 nt) IS186 repeat region
?REL606 4210241 = 43 (0.720)intergenic (+84/+49) yjbC/yjbD 23S rRNA pseudouridine synthase/hypothetical protein