Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A56 F1 I1 R1
|
16 |
62.6 |
4005459 |
97.2% |
3893306 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,162,569 |
A→T |
I572F (ATC→TTC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,162,569 | 0 | A | T | 98.7%
| 294.3
/ ‑3.7
| 74 | I572F (ATC→TTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (35/38); total (35/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 |
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641
|
cGAGACGTACACCCGACTCACTATGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 2:688751/1‑76 (MQ=255)
cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 1:682146/1‑76 (MQ=255)
cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 2:225638/1‑76 (MQ=255)
cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 2:1161305/1‑76 (MQ=255)
agaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAAc < 2:778928/76‑1 (MQ=255)
tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAActctct < 2:349393/76‑1 (MQ=255)
acccccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTg < 2:1632125/73‑1 (MQ=255)
acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTg < 2:1690448/76‑1 (MQ=255)
cccccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGt < 2:657926/74‑1 (MQ=255)
cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGCACATCGGGCTGTTCAACTCTCTGt > 1:968339/1‑76 (MQ=255)
cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGt < 1:173247/76‑1 (MQ=255)
ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCg < 2:1918022/76‑1 (MQ=255)
aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCgtgt < 2:778927/76‑1 (MQ=255)
aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCgtgt < 2:876531/76‑1 (MQ=255)
cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTa < 2:842658/76‑1 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACg > 2:1204204/1‑76 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACg < 1:542811/76‑1 (MQ=255)
aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGcaca > 2:469205/1‑76 (MQ=255)
aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGcaca < 1:1181281/76‑1 (MQ=255)
cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAg < 1:260619/76‑1 (MQ=255)
cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAg < 1:69483/76‑1 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGa > 2:962088/1‑76 (MQ=255)
gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGAc < 2:696018/76‑1 (MQ=255)
tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACt > 2:247176/1‑76 (MQ=255)
cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGCCTa < 2:1121226/76‑1 (MQ=255)
cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTa > 1:323404/1‑76 (MQ=255)
gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTaa < 2:1875481/76‑1 (MQ=255)
gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTaa < 1:74970/76‑1 (MQ=255)
gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACg > 1:1054739/1‑76 (MQ=255)
tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:1338906/1‑76 (MQ=255)
tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:1519865/1‑76 (MQ=255)
tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa < 1:1526975/76‑1 (MQ=255)
tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 1:1012789/1‑76 (MQ=255)
aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 2:997391/1‑76 (MQ=255)
gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa > 2:178747/1‑76 (MQ=255)
gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:513744/76‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 2:34406/1‑76 (MQ=255)
ctaaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:494841/3‑76 (MQ=255)
cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 2:1196839/76‑1 (MQ=255)
aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc < 2:294615/76‑1 (MQ=255)
aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCt < 2:1604464/76‑1 (MQ=255)
gAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc > 2:1097730/1‑76 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 1:1310502/1‑76 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:876062/76‑1 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:1062955/76‑1 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 1:340779/76‑1 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg < 2:450911/76‑1 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:1451833/76‑1 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 2:624781/76‑1 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:214618/76‑1 (MQ=255)
gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 2:866837/1‑76 (MQ=255)
gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc < 2:1851100/76‑1 (MQ=255)
gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc < 1:676054/76‑1 (MQ=255)
naGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 1:468519/2‑76 (MQ=255)
aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg > 2:1082964/1‑76 (MQ=255)
aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg > 2:1906926/1‑76 (MQ=255)
cGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg > 1:387903/1‑76 (MQ=255)
cGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg < 2:1449581/76‑1 (MQ=255)
gAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt > 1:1711300/1‑76 (MQ=255)
gAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt > 1:489360/1‑76 (MQ=255)
aaCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa > 1:1738463/1‑76 (MQ=255)
aCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa > 1:1200629/1‑76 (MQ=255)
aCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa < 2:1568698/76‑1 (MQ=255)
cATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa > 2:551427/1‑76 (MQ=255)
aTCGGTCTGTTCAACTCTCTGTCCGTGTACGGACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 1:1131874/76‑1 (MQ=255)
aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 2:450360/76‑1 (MQ=255)
aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg > 2:1338507/1‑76 (MQ=255)
aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg > 2:723670/1‑76 (MQ=255)
aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 1:361532/76‑1 (MQ=255)
cGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg > 1:106608/1‑76 (MQ=255)
cGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg < 1:1723056/76‑1 (MQ=255)
gTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCTTAAAGTGAc > 1:563179/1‑76 (MQ=255)
cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 1:1277145/76‑1 (MQ=255)
cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg > 1:961671/1‑76 (MQ=255)
cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 1:684812/76‑1 (MQ=255)
cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCCTGAGACTCCGTATCGTAAAGTGACCg > 1:1255337/1‑76 (MQ=255)
|
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
