Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A61 F1 I1 R1
|
19 |
50.7 |
3232285 |
96.8% |
3128851 |
74.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,162,569 |
A→T |
I572F (ATC→TTC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,162,569 | 0 | A | T | 100.0%
| 245.4
/ NA
| 60 | I572F (ATC→TTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (32/28); total (32/28) |
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACC > REL606/4162497‑4162640
|
cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTc < 2:555004/75‑1 (MQ=255)
aCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAAct < 2:1307470/75‑1 (MQ=255)
cGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAActc < 2:1019745/75‑1 (MQ=255)
gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAActct < 1:915132/75‑1 (MQ=255)
tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAActctc < 2:1602187/75‑1 (MQ=255)
acCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGt > 1:39839/1‑75 (MQ=255)
acCCGACTCACTACGGTCGCGTATGGCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGt > 1:39841/1‑75 (MQ=255)
cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTc > 1:1088548/1‑75 (MQ=255)
cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCg < 2:1259586/75‑1 (MQ=255)
tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTa > 1:835481/1‑75 (MQ=255)
tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTa < 1:1467825/75‑1 (MQ=255)
cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGc > 2:131885/1‑75 (MQ=255)
cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGc > 1:922603/1‑75 (MQ=255)
cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGcaca < 2:112979/75‑1 (MQ=255)
gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGa > 1:938186/1‑75 (MQ=255)
tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGAc < 2:1507922/75‑1 (MQ=255)
cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACt < 2:147271/75‑1 (MQ=255)
cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTaa < 1:318320/75‑1 (MQ=255)
cgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTaa < 1:1399250/75‑1 (MQ=255)
gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAAc > 1:862400/1‑75 (MQ=255)
aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:179017/1‑75 (MQ=255)
tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 1:195869/1‑75 (MQ=255)
gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 2:147415/75‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:1253176/75‑1 (MQ=255)
ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 2:861687/1‑75 (MQ=255)
cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg < 2:1313192/75‑1 (MQ=255)
cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 1:1226034/1‑75 (MQ=255)
aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 2:932930/1‑75 (MQ=255)
aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc < 2:1435375/75‑1 (MQ=255)
tCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCt < 1:776566/75‑1 (MQ=255)
gAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 2:1320886/1‑75 (MQ=255)
aaaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc < 1:645062/75‑1 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt > 2:956162/1‑75 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt > 1:1242251/1‑75 (MQ=255)
aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt > 2:1198035/1‑75 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 1:426208/75‑1 (MQ=255)
aCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 1:210372/1‑75 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga > 2:361771/1‑75 (MQ=255)
cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga > 1:430524/1‑75 (MQ=255)
cTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga > 1:1573367/1‑75 (MQ=255)
tGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc < 2:1300074/75‑1 (MQ=255)
tGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc < 2:1483524/75‑1 (MQ=255)
gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt > 1:1615746/1‑75 (MQ=255)
gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 1:840450/75‑1 (MQ=255)
aaGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACGGACTAACGAATACGGCTTCCTTGAGACTc > 1:519647/1‑75 (MQ=255)
aaGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 1:923594/1‑75 (MQ=255)
aaGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 2:1514451/1‑75 (MQ=255)
aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 1:460117/1‑75 (MQ=255)
aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 2:208679/1‑75 (MQ=255)
aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 1:1477732/1‑75 (MQ=255)
ggTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg < 1:426506/75‑1 (MQ=255)
gTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 1:384599/75‑1 (MQ=255)
tCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa < 2:110532/75‑1 (MQ=255)
aaCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt < 1:861687/75‑1 (MQ=255)
aCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa < 2:1088548/75‑1 (MQ=255)
aCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa < 1:752889/75‑1 (MQ=255)
tCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 1:592682/75‑1 (MQ=255)
ggTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAggg > 1:152993/1‑73 (MQ=255)
ggTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg > 1:815962/1‑75 (MQ=255)
gTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa > 2:950989/1‑75 (MQ=255)
tCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAc > 1:924050/1‑75 (MQ=255)
cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc > 1:1446373/1‑75 (MQ=255)
|
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACC > REL606/4162497‑4162640
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
