Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A124 F1 I1 R1
|
18 |
228.8 |
14151462 |
95.4% |
13500494 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,338,860 |
A→C |
R230S (AGA→AGC) |
yhdG → |
tRNA‑dihydrouridine synthase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,338,860 | 0 | A | C | 99.2%
| 1093.8
/ ‑5.0
| 264 | R230S (AGA→AGC) | yhdG | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (103/159); total (104/160) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 284 total aligned reads displayed.
CCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAG > REL606/3338785‑3338932
|
cGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGccc < 1:2169186/76‑1 (MQ=255)
gCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGcccc > 1:3566607/1‑76 (MQ=255)
cTTAACGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCCCAGCTCAGGGAAGCCCCt < 1:529894/76‑1 (MQ=255)
cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCCGGGAAGCCCCt > 1:1360314/1‑76 (MQ=255)
cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCt < 1:5772299/76‑1 (MQ=255)
cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCt < 1:1261973/76‑1 (MQ=255)
cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCt < 2:4714422/76‑1 (MQ=255)
ttAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATTATAAGGAACGCCGCTCAGGGAACCCCCtc > 2:5399173/1‑75 (MQ=255)
tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTgg < 2:1137035/76‑1 (MQ=255)
tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTgg < 1:3843776/76‑1 (MQ=255)
tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTgg > 1:4834080/1‑76 (MQ=255)
aaaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGa < 1:2016616/76‑1 (MQ=255)
aaaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGa > 1:6353222/1‑76 (MQ=255)
aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGAt < 2:1308984/76‑1 (MQ=255)
aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGAt < 2:6851786/76‑1 (MQ=255)
aGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCCGGGAAGCCCCTGGATc > 2:5925281/1‑76 (MQ=255)
cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCttt > 2:1735779/1‑76 (MQ=255)
cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCttt > 2:7025376/1‑76 (MQ=255)
agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTc < 2:6544407/76‑1 (MQ=255)
agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTc < 1:625824/76‑1 (MQ=255)
agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTc < 1:1855243/76‑1 (MQ=255)
gCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCggg > 1:5565238/1‑76 (MQ=255)
gCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCggg < 1:1596930/76‑1 (MQ=255)
cTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGa > 1:3584750/1‑76 (MQ=255)
cTGTGCTCGACTATACAGGGGCGGAAGCCCCGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCCTTCgggg > 1:4598942/1‑75 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGGCGCGCCACTCCGGGAAGGCCCTGGATCTTTCCGGgaa > 2:6496714/1‑76 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGaaa > 1:2330875/1‑76 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGaaa > 1:6423064/1‑76 (MQ=255)
tgCTCGACTATACAGGTGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAAt > 1:4040421/1‑76 (MQ=255)
tgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGGAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAAt > 1:6089069/1‑76 (MQ=255)
tgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAAt < 1:4002934/76‑1 (MQ=255)
tCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCa < 1:5917904/76‑1 (MQ=255)
tCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGCGAAATCCa < 1:4173624/76‑1 (MQ=255)
cGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAg < 2:2133597/76‑1 (MQ=255)
cGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAg < 1:4330743/76‑1 (MQ=255)
gACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATACAGc > 1:5341992/1‑76 (MQ=255)
aCTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCa < 2:419459/76‑1 (MQ=255)
aCTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCa < 2:6631229/76‑1 (MQ=255)
cTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCAt < 2:817761/76‑1 (MQ=255)
tataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCAtt < 1:2265296/76‑1 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTa < 1:56126/76‑1 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTa > 2:6649378/1‑76 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTa < 2:5860743/76‑1 (MQ=255)
taCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTAt < 1:2945532/76‑1 (MQ=255)
cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCt > 1:5429651/1‑76 (MQ=255)
cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCt < 1:6565010/76‑1 (MQ=255)
cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCt < 2:4064059/76‑1 (MQ=255)
aGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTg < 1:6127212/76‑1 (MQ=255)
aGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTg < 2:1497629/76‑1 (MQ=255)
gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 2:4868479/76‑1 (MQ=255)
gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 1:728076/76‑1 (MQ=255)
gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 2:2366220/76‑1 (MQ=255)
ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac > 1:824731/1‑76 (MQ=255)
ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac > 1:5422678/1‑76 (MQ=255)
ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 1:2291176/76‑1 (MQ=255)
ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 1:3126858/76‑1 (MQ=255)
cGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGacac > 1:1799086/1‑76 (MQ=255)
ggATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACt < 2:3747037/76‑1 (MQ=255)
aTGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg > 2:5574817/1‑76 (MQ=255)
tGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg > 1:750291/1‑76 (MQ=255)
gCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg > 1:1515691/1‑76 (MQ=255)
gCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg > 2:4890854/1‑76 (MQ=255)
cccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGa < 2:5791506/76‑1 (MQ=255)
cccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGa < 2:2533691/76‑1 (MQ=255)
ccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg > 2:4512570/1‑76 (MQ=255)
ccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:4521126/76‑1 (MQ=255)
cTGATGATAGGCCGCGCAGCTCAGGGAAGCGCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 2:3888146/1‑76 (MQ=255)
cTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 1:5218197/1‑76 (MQ=255)
cTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 2:4565205/1‑76 (MQ=255)
tgatgaTAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 2:2592694/1‑76 (MQ=255)
atgatAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGATACTGGGGAGTtgc > 1:3398441/1‑76 (MQ=255)
atgatAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgc < 2:4958761/76‑1 (MQ=255)
tgatAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgct > 1:3701913/1‑76 (MQ=255)
atAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctgc > 1:4089139/1‑76 (MQ=255)
atAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctgc > 2:4010811/1‑76 (MQ=255)
aGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccc < 2:4296060/76‑1 (MQ=255)
gCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 2:5733752/76‑1 (MQ=255)
gCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 1:1892477/76‑1 (MQ=255)
gCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 1:268975/76‑1 (MQ=255)
gcgcAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCt < 2:2697198/76‑1 (MQ=255)
cgcAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTg < 1:6797181/76‑1 (MQ=255)
cgcAGCCCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTg < 1:3344179/76‑1 (MQ=255)
cAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGcc > 1:885684/1‑76 (MQ=255)
aGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:3940958/1‑76 (MQ=255)
aGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:337198/1‑76 (MQ=255)
aGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:5986893/1‑76 (MQ=255)
gCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCtt < 2:3551048/76‑1 (MQ=255)
cTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt < 2:5247036/76‑1 (MQ=255)
cTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt < 1:1011216/76‑1 (MQ=255)
cAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:4822275/1‑76 (MQ=255)
cAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 1:2535818/1‑76 (MQ=255)
cAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:6822776/1‑76 (MQ=255)
aGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGc > 2:3079842/1‑76 (MQ=255)
gggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCa < 1:2820597/76‑1 (MQ=255)
ggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCag < 1:4656222/76‑1 (MQ=255)
ggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCag < 2:1997552/76‑1 (MQ=255)
ggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTNGGCag < 2:2253035/76‑1 (MQ=255)
aaGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag < 1:1043286/76‑1 (MQ=255)
aaGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag < 2:6321934/76‑1 (MQ=255)
aaGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag < 2:4622584/76‑1 (MQ=255)
|
CCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAG > REL606/3338785‑3338932
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
