Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A67 F1 I1 R1
|
17 |
65.0 |
4212288 |
95.3% |
4014310 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,841 |
A→T |
I209F (ATC→TTC) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,841 | 0 | A | T | 97.4%
| 295.5
/ ‑2.3
| 76 | I209F (ATC→TTC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (35/39); total (35/41) |
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTG > REL606/3928771‑3928913
|
cATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTcgac < 2:565747/76‑1 (MQ=255)
aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTcgacg > 1:1912712/1‑76 (MQ=255)
gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGaa > 1:965265/1‑76 (MQ=255)
ctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAAc > 2:881613/1‑76 (MQ=255)
tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACg < 2:1971261/76‑1 (MQ=255)
tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACg < 1:991212/76‑1 (MQ=255)
tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACg > 1:666893/1‑76 (MQ=255)
ctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTc > 1:1120197/1‑76 (MQ=255)
ctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTc < 1:1920484/76‑1 (MQ=255)
gcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCg > 2:78348/1‑76 (MQ=255)
aGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGga < 1:1175760/76‑1 (MQ=255)
aCATTGCTCAGAGCATTGCTTACAACCCCCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga < 2:1291113/76‑1 (MQ=255)
aCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga > 1:1759557/1‑76 (MQ=255)
aCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga < 2:632559/76‑1 (MQ=255)
aCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga > 2:701769/1‑76 (MQ=255)
aTTGCTCAGAGCCTTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGaagaag < 1:1873285/76‑1 (MQ=255)
ttGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGt < 1:463984/76‑1 (MQ=255)
ttGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGt < 1:955203/76‑1 (MQ=255)
tGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTa < 2:1458878/76‑1 (MQ=255)
gCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTaa < 2:1072543/76‑1 (MQ=255)
ccTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTaa > 1:2101567/2‑76 (MQ=255)
cTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAAc > 2:1527911/1‑76 (MQ=255)
tCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAAcc < 2:84234/76‑1 (MQ=255)
cAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCg > 1:2077725/1‑76 (MQ=255)
cAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCg < 2:1566565/76‑1 (MQ=255)
gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCgag < 1:1625432/76‑1 (MQ=255)
agCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCgaga < 2:1912712/76‑1 (MQ=255)
cATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATg > 2:303647/1‑76 (MQ=255)
aTTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGc < 1:1777298/76‑1 (MQ=255)
ttGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCa > 1:2099362/1‑76 (MQ=255)
gCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGc > 1:884974/1‑76 (MQ=255)
gCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGc < 2:965265/76‑1 (MQ=255)
cTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCg > 1:453394/1‑76 (MQ=255)
cTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCg < 2:1135259/76‑1 (MQ=255)
tACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTc < 1:195234/76‑1 (MQ=255)
cAACCCCCCGGATTGTGTGCTGATTGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTg < 1:1270746/76‑1 (MQ=255)
cAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTg < 1:445366/76‑1 (MQ=255)
accaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 2:40173/76‑1 (MQ=255)
accaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 2:307749/76‑1 (MQ=255)
ccaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTa > 2:99342/1‑76 (MQ=255)
ccaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTa < 2:2037200/76‑1 (MQ=255)
caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:1741422/76‑1 (MQ=255)
caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:545153/76‑1 (MQ=255)
caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:728676/76‑1 (MQ=255)
caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:1743744/76‑1 (MQ=255)
ccGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAg > 1:197659/1‑76 (MQ=255)
ccGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAg < 2:1501004/76‑1 (MQ=255)
ccGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAg > 2:787043/1‑76 (MQ=255)
gATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTg > 2:1548953/1‑76 (MQ=255)
gATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTg > 2:1021749/1‑76 (MQ=255)
ttGTGTGCTTATGGTTCTGCTGATCCACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGaa < 2:1847559/76‑1 (MQ=255)
ttGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGaa < 1:665906/76‑1 (MQ=255)
tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg > 1:317296/1‑76 (MQ=255)
tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg < 1:268554/76‑1 (MQ=255)
tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg > 1:502833/1‑76 (MQ=255)
tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg > 1:15773/1‑76 (MQ=255)
tgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgt > 2:442176/1‑76 (MQ=255)
tGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGc < 2:371228/76‑1 (MQ=255)
tGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGCGAAGTTGTTGc > 1:365284/1‑76 (MQ=255)
aTGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCtt > 1:320217/1‑76 (MQ=255)
aTGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCtn < 1:815438/76‑2 (MQ=255)
ttgTTCTTCTGTTCGACGAACGGCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTc < 2:994786/74‑1 (MQ=255)
tGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTc > 1:1733213/1‑76 (MQ=255)
ggTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCt < 1:239477/76‑1 (MQ=255)
ggTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCt < 2:838055/76‑1 (MQ=255)
ggTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCt > 2:1754968/1‑76 (MQ=255)
gTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTa > 2:181597/1‑76 (MQ=255)
ttCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTAc > 2:887939/1‑76 (MQ=255)
ttCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTAc > 2:966672/1‑76 (MQ=255)
tCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTAcc > 1:1494798/1‑76 (MQ=255)
ctgctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCt < 1:1479695/76‑1 (MQ=255)
ctgctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCt < 1:1479683/76‑1 (MQ=255)
gctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCttt > 2:71635/1‑76 (MQ=255)
ctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTCCCTTTg > 2:1949667/1‑76 (MQ=255)
ctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTg < 2:676825/76‑1 (MQ=255)
ctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTg > 2:134255/1‑76 (MQ=255)
|
CATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTG > REL606/3928771‑3928913
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
