Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A57 F1 I1 R1
|
16 |
65.9 |
4148770 |
97.1% |
4028455 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,161,969 |
C→T |
P372S (CCT→TCT) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,161,969 | 0 | C | T | 100.0%
| 209.5
/ NA
| 56 | P372S (CCT→TCT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (39/17); total (39/17) |
TCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATG > REL606/4161897‑4162041
|
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg > 1:585124/1‑76 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg > 1:986145/1‑76 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg > 1:1333789/1‑76 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:570470/76‑1 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg > 2:979362/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg < 1:92344/76‑1 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:1702698/1‑76 (MQ=255)
gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg > 2:1443350/1‑76 (MQ=255)
aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGa > 1:1865762/1‑76 (MQ=255)
ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgcc > 1:115098/1‑76 (MQ=255)
cTTACGTGCCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccg < 2:898829/76‑1 (MQ=255)
ttACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgc > 2:344307/1‑76 (MQ=255)
ttACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgc > 2:1630926/1‑76 (MQ=255)
tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgcc > 1:1290207/1‑76 (MQ=255)
tgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACt > 1:1076845/1‑76 (MQ=255)
gtCGACCCAACTAACGACCGGCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTc > 2:1536208/1‑76 (MQ=255)
cGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGt > 1:1045477/1‑76 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg > 2:127724/1‑76 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg > 2:267156/1‑76 (MQ=255)
cccccAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg < 2:1199373/74‑1 (MQ=255)
cAACTAACGACCGTCTTAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagc < 1:508490/76‑1 (MQ=255)
cTAACGCCCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagcagc < 1:1169900/76‑1 (MQ=255)
cTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagcagc > 1:1243121/1‑76 (MQ=255)
tAACGACCGTCTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCg < 1:42108/76‑1 (MQ=255)
tAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCg < 2:1712159/76‑1 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 1:2024155/1‑76 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:1233096/1‑76 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:799552/1‑76 (MQ=255)
gACCGTCTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 1:1024480/76‑1 (MQ=255)
gACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGCGCAGCCGCGGaaa > 2:732368/1‑76 (MQ=255)
caCCGTGTGAGCGCACTGGTAGAAATCCCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 2:727371/75‑1 (MQ=255)
cGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGcc > 2:1656965/1‑76 (MQ=255)
gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 1:2048975/76‑1 (MQ=255)
tGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt > 2:720596/1‑76 (MQ=255)
tGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt > 2:1027053/1‑76 (MQ=255)
gAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc < 2:893891/76‑1 (MQ=255)
cgcAGTGGTAGAAATCTCCCACATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag < 2:354800/76‑1 (MQ=255)
cACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGaa > 1:58882/1‑76 (MQ=255)
tGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCt < 2:1699460/76‑1 (MQ=255)
tAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGtt > 2:1491017/1‑76 (MQ=255)
gAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttct < 2:600639/76‑1 (MQ=255)
gAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttct > 2:1735056/1‑76 (MQ=255)
naTCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttcttc > 1:1249609/2‑76 (MQ=255)
aTCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTtcttct > 2:1664132/1‑76 (MQ=255)
tCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTtctc > 1:1106591/1‑76 (MQ=255)
cGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg > 1:599803/1‑76 (MQ=255)
gCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGa < 1:704687/76‑1 (MQ=255)
cATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc < 2:165890/76‑1 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 2:1860539/1‑76 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 1:537980/1‑76 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 2:1319173/1‑76 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 2:1151272/1‑76 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 1:794358/1‑76 (MQ=255)
atgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt > 1:1999617/1‑76 (MQ=255)
tgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTGTTCTCCGAAGACCGTTa < 2:991768/76‑1 (MQ=255)
tgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTa < 2:1339449/76‑1 (MQ=255)
tgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTa > 2:960760/1‑76 (MQ=255)
cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg < 1:1568274/76‑1 (MQ=255)
cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg < 1:1771795/76‑1 (MQ=255)
cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg < 2:841115/76‑1 (MQ=255)
cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg > 1:416629/1‑76 (MQ=255)
cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg < 1:762098/76‑1 (MQ=255)
|
TCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATG > REL606/4161897‑4162041
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
