Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A110 F1 I1 R1
|
14 |
278.2 |
17968888 |
92.5% |
16621221 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,161,969 |
C→T |
P372S (CCT→TCT) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,161,969 | 0 | C | T | 98.5%
| 760.8
/ ‑0.9
| 196 | P372S (CCT→TCT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (157/36); total (158/38) |
| Fisher's exact test for biased strand distribution p-value = 9.67e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 |
Only 100 of 290 total aligned reads displayed.
CTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATG > REL606/4161896‑4162041
|
ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct < 2:7495637/76‑1 (MQ=255)
ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct > 1:1487448/1‑76 (MQ=255)
ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct < 2:6769286/76‑1 (MQ=255)
ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct > 1:5123456/1‑76 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:2119229/76‑1 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 1:8393618/76‑1 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:8306102/76‑1 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:5370964/76‑1 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:4365176/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:409712/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg < 2:8369610/76‑1 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:7969618/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 2:2864676/1‑76 (MQ=255)
gAAACCTTACGTGTCGGCCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg < 1:3342967/76‑1 (MQ=255)
gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg > 2:5951864/1‑76 (MQ=255)
gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg < 2:7422903/76‑1 (MQ=255)
gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCAGGCg < 2:432344/76‑1 (MQ=255)
aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGa < 1:7006206/76‑1 (MQ=255)
aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGa > 2:4407586/1‑76 (MQ=255)
aCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAgc > 1:1081253/1‑76 (MQ=255)
cTTCCGGGTCGCCCAAACTAACGCCCGTCGGAGCGCACTGGTAAAAATCTCCCGCATGATGCGCTCGGGGGAgccg < 2:4624239/76‑1 (MQ=255)
cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccg > 2:1469719/1‑76 (MQ=255)
cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGAGGCGCTCTGGCGAgcca < 2:4212130/76‑2 (MQ=255)
ttACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgc > 1:2526119/1‑76 (MQ=255)
tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgcc > 1:2899108/1‑76 (MQ=255)
cGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGa > 2:7613247/1‑76 (MQ=255)
cGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCAGa > 2:2705907/1‑76 (MQ=255)
tgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACt > 2:6608699/1‑76 (MQ=255)
gtCGACCCAACTAACGACCGTCTGGGCGCACTGGTAAAAATCTACCGCATGATGCGCTCTGGGGAGCCGCCGACTc < 2:865412/76‑1 (MQ=255)
gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTc > 1:56398/1‑76 (MQ=255)
gccGACACAACTAACGACCGTCTGAGCGCACGGTTAGAAACCTACCGCATGTTCCGCTCGGGGGAGCCGCCGACTc < 2:6566645/74‑1 (MQ=255)
gACCCAACTACCGACCGTCGGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTg < 2:4845578/76‑1 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg > 2:5690041/1‑76 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg > 2:616046/1‑76 (MQ=255)
caCCCAATTAAGGACCGCCTGAGCCCGAGGTAAAAAATCTCCGGCATAATGCGCGCGGGGGAGCCGCCGACTCGTg < 2:235153/75‑1 (MQ=255)
cccAACTACCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGaa < 1:1606716/76‑1 (MQ=255)
ccACCTAACGACGGTCTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAag < 2:7860487/76‑1 (MQ=255)
ccAACTAACGACCGTCTGGGCGCCCTGGTAAAAATCTCCCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTGAag < 2:3128273/76‑1 (MQ=255)
cAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagc > 1:6512640/1‑76 (MQ=255)
cAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagc > 1:1247384/1‑76 (MQ=255)
aaCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagca > 2:1619000/1‑76 (MQ=255)
aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagcag > 1:7678235/1‑76 (MQ=255)
aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCACTCTGGCGAGCCGCCGACTCGTGAagcag > 1:211170/1‑76 (MQ=255)
cTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagcagc > 2:6175614/1‑76 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 1:2639948/1‑76 (MQ=255)
cGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaa > 1:7258838/1‑76 (MQ=255)
gcccGTCTGAGCGCCCTGGTAGAAATCTACCGCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 1:5231508/74‑1 (MQ=255)
gcccGTCTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 1:7829139/74‑1 (MQ=255)
gACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 1:3091259/1‑76 (MQ=255)
gACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 2:1563644/1‑76 (MQ=255)
cccGTCTGAGCGCCCTGGTAGAAATCTACCGCATGATGCGTTCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 1:2348395/75‑1 (MQ=255)
cccGTCTGAGCGCATGGGTAGAAATCTCCCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 2:3466271/75‑1 (MQ=255)
ccGTCTGAGCGCCGTGGTAGAAATCTCCCGCATGATGCGCGCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGc < 2:6015129/76‑1 (MQ=255)
cGTCTGCGCGCGTGGGAAGAAATCTCCGGCATGATGCCCTCTGGCGGGCCGCCGACTCGTGAAGCAGCGGAAAGcc < 1:4811943/76‑1 (MQ=255)
cGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGcc > 1:4191984/1‑76 (MQ=255)
gTCTGAGCGCCCTGTTAGAAACCTCCCGCATGATGCGCGAGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:1199202/76‑1 (MQ=255)
gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt > 2:883991/1‑76 (MQ=255)
gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:4362766/76‑1 (MQ=255)
tCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTg > 1:7376714/1‑76 (MQ=255)
cttaGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGt > 2:1233967/4‑76 (MQ=255)
cggAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGt < 1:7235263/74‑1 (MQ=255)
tGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 1:4945144/76‑1 (MQ=255)
tGAGCGCACTGGTAAAATTCTCCCGAATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 1:3697682/76‑1 (MQ=255)
ggAGCCCCCTGGAAGAAATCTACCGCATGATGCGCTTGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 2:3913649/75‑1 (MQ=255)
gAGCGCACTGGTAAAAATCTACCGCAGGAGGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc < 2:2561688/76‑1 (MQ=255)
aGCGCCCGGGTAGAAATCTCCCGCATGAGGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCg < 1:7344929/76‑1 (MQ=255)
cgcACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag > 2:564410/1‑76 (MQ=255)
cgcACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag > 1:1808161/1‑76 (MQ=255)
gcACGGGTAGAAATCTCCCGCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgaga < 1:453785/76‑1 (MQ=255)
ccTGGAAAAAACCTCCCGCATGAGGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAc < 2:8430222/75‑1 (MQ=255)
aCTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAc > 2:1993780/1‑76 (MQ=255)
cTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAcc > 1:8319469/1‑76 (MQ=255)
aGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttc > 1:4605819/1‑76 (MQ=255)
aGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttc > 1:951139/1‑76 (MQ=255)
atttctcCCACATGATGCGCTCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 2:1755571/73‑1 (MQ=255)
aaaTCTCCCGCGTGAGGCGTCTGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 1:5954464/76‑1 (MQ=255)
aaaTCTACCTCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 2:2235395/76‑1 (MQ=255)
aaaTCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt > 1:673652/1‑76 (MQ=255)
aaaTCTACCCCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 1:6469420/76‑1 (MQ=255)
tCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGACGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTtctc > 1:5322092/1‑76 (MQ=255)
cTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTcc > 1:7520039/1‑76 (MQ=255)
tACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCg > 2:691119/1‑76 (MQ=255)
aCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGa > 1:8618722/1‑76 (MQ=255)
aCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGa > 1:6797428/1‑76 (MQ=255)
ccGCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGaa < 2:802356/76‑1 (MQ=255)
ccGAATAATGCCCTCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGaa < 2:3824755/76‑1 (MQ=255)
cGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg < 1:8871808/76‑1 (MQ=255)
cGCATGAGGCCCTGTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg < 2:2457069/76‑1 (MQ=255)
cATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc > 1:295645/1‑76 (MQ=255)
cATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc > 2:5082482/1‑76 (MQ=255)
cATGAGGCCCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc < 1:3394089/76‑1 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 2:6393136/1‑76 (MQ=255)
atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 1:8458615/1‑76 (MQ=255)
gatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGt > 1:868358/1‑76 (MQ=255)
atgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt < 1:3872000/76‑1 (MQ=255)
atgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt > 2:5191574/1‑76 (MQ=255)
agccgcTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt < 1:5688705/73‑1 (MQ=255)
tgCGCTCTGGCGAGCCGCCGACTCGTAAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTa > 2:7283092/1‑76 (MQ=255)
gcgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTAt < 1:102205/76‑1 (MQ=255)
cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg > 2:3217162/1‑76 (MQ=255)
|
CTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATG > REL606/4161896‑4162041
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
