Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A138 F1 I1 R1
|
17 |
163.5 |
10262604 |
95.7% |
9821312 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,941,586 |
G→A |
V396M (GTG→ATG) |
wecF → |
putative enterobacterial common antigen polymerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,941,586 | 0 | G | A | 99.4%
| 690.2
/ ‑6.2
| 164 | V396M (GTG→ATG) | wecF | putative enterobacterial common antigen polymerase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (90/73); total (91/73) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 165 total aligned reads displayed.
CAACCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCATGTC > REL606/3941513‑3941658
|
cAACCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATg < 2:244998/76‑1 (MQ=255)
aaCCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGc < 2:3702383/76‑1 (MQ=255)
aaCCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGc < 2:4970837/76‑1 (MQ=255)
ccGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTg < 2:2684212/76‑1 (MQ=255)
ccGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTg < 1:978327/76‑1 (MQ=255)
cgcgAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTgg < 1:5015861/76‑1 (MQ=255)
cgAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTggag > 2:4371194/1‑74 (MQ=255)
gaCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGt > 2:3909897/1‑76 (MQ=255)
gaCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGt < 2:325668/76‑1 (MQ=255)
gaCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGt > 1:1364067/1‑76 (MQ=255)
aCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTg < 1:1700972/76‑1 (MQ=255)
cTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGa > 2:1247612/1‑76 (MQ=255)
tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa < 1:4936749/76‑1 (MQ=255)
tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa < 1:4356055/76‑1 (MQ=255)
tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa > 2:3814648/1‑76 (MQ=255)
tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa < 2:1529706/76‑1 (MQ=255)
aaTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAg < 1:3090544/76‑1 (MQ=255)
aaTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAg < 2:695900/76‑1 (MQ=255)
aTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAgg < 1:1876887/76‑1 (MQ=255)
aTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAgg > 2:3432852/1‑76 (MQ=255)
cGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGc > 1:2709680/1‑76 (MQ=255)
cGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGc > 2:4031086/1‑76 (MQ=255)
gCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCt < 2:1486057/76‑1 (MQ=255)
gCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCt < 1:3728674/76‑1 (MQ=255)
gCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCt > 2:2838348/1‑76 (MQ=255)
cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg > 1:399729/1‑76 (MQ=255)
cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg < 2:3025130/76‑1 (MQ=255)
cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg < 2:2669298/76‑1 (MQ=255)
cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg < 1:3481290/76‑1 (MQ=255)
cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg > 1:3162498/1‑76 (MQ=255)
cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg > 1:287484/1‑76 (MQ=255)
tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg < 2:239962/76‑1 (MQ=255)
tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg > 1:616032/1‑76 (MQ=255)
tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg > 1:4894828/1‑76 (MQ=255)
tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg < 1:4320095/76‑1 (MQ=255)
ataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGa < 2:3817392/76‑1 (MQ=255)
ataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGa > 1:3880635/1‑76 (MQ=255)
ataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGa > 2:4401655/1‑76 (MQ=255)
taAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGAt > 1:4158648/1‑76 (MQ=255)
taAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGAt > 2:4224225/1‑76 (MQ=255)
aaGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGGtt > 1:431314/1‑76 (MQ=255)
aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc > 1:4680721/1‑76 (MQ=255)
aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc < 1:1052940/76‑1 (MQ=255)
aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc < 1:3621516/76‑1 (MQ=255)
aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc < 1:2745273/76‑1 (MQ=255)
cTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGtt > 1:2816884/1‑76 (MQ=255)
cTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGtt < 2:1185525/76‑1 (MQ=255)
cTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGtt < 2:2397785/76‑1 (MQ=255)
tGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGttt > 1:82195/1‑76 (MQ=255)
gCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTg < 2:2240819/76‑1 (MQ=255)
cGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGt > 2:3482370/1‑76 (MQ=255)
cGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGt > 2:1460366/1‑76 (MQ=255)
atatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtct > 1:3455067/1‑76 (MQ=255)
atatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtct < 2:5016568/76‑1 (MQ=255)
atatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtct > 1:1849446/1‑76 (MQ=255)
tatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtctc < 1:2272595/76‑1 (MQ=255)
atTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCa < 2:775152/76‑1 (MQ=255)
ttACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAc < 2:4508399/76‑1 (MQ=255)
ttACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAc < 2:4237571/76‑1 (MQ=255)
tACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcg < 1:74601/76‑1 (MQ=255)
acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 2:3081705/1‑76 (MQ=255)
acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 2:4728655/1‑76 (MQ=255)
acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 1:2148954/1‑76 (MQ=255)
acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 2:4657447/1‑76 (MQ=255)
cacaGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgcg < 2:129401/76‑1 (MQ=255)
aGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTgg > 2:4498483/1‑76 (MQ=255)
tttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTc > 1:3392659/1‑76 (MQ=255)
ttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCt > 1:2635064/1‑76 (MQ=255)
ttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCt > 1:1805413/1‑76 (MQ=255)
ttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCt < 1:1736620/76‑1 (MQ=255)
cTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCttt > 1:958988/1‑76 (MQ=255)
tGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGGCtttt > 1:1326276/1‑76 (MQ=255)
gCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCttttt > 1:2117193/1‑76 (MQ=255)
gCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCttttt > 2:4972825/1‑76 (MQ=255)
gCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGCCttttt > 1:4540972/1‑76 (MQ=255)
cTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCtttttt < 2:3647609/76‑1 (MQ=255)
cTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCtttttt < 1:1104105/76‑1 (MQ=255)
tCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTAt > 2:3854787/1‑76 (MQ=255)
gCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTgg > 2:2209438/1‑76 (MQ=255)
gATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTc > 2:943843/1‑76 (MQ=255)
gATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTc < 1:1578041/76‑1 (MQ=255)
gATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTc > 2:1783773/1‑76 (MQ=255)
aTCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCt > 2:4399082/1‑76 (MQ=255)
aTCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCt > 1:2940499/1‑76 (MQ=255)
aTCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCt > 1:4661707/1‑76 (MQ=255)
tCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCtt < 2:1723739/76‑1 (MQ=255)
tCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCtt < 1:1154716/76‑1 (MQ=255)
ttCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCg < 2:150844/76‑1 (MQ=255)
ttCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCg > 1:2564779/1‑76 (MQ=255)
ttCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCg > 1:1243065/1‑76 (MQ=255)
tCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCgg < 1:4783501/76‑1 (MQ=255)
tCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCgg > 1:2893292/1‑76 (MQ=255)
tCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCgg < 1:1306213/76‑1 (MQ=255)
cAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGgc > 2:360904/1‑76 (MQ=255)
cAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCATGGTCTTCGgc > 2:2954857/1‑76 (MQ=255)
aaTATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGgcg > 1:4550759/1‑76 (MQ=255)
aaTATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGgcg > 1:4209075/1‑76 (MQ=255)
tatGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCTCa < 1:2045147/76‑1 (MQ=255)
tatGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCa > 1:4236813/1‑76 (MQ=255)
tatGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCa < 2:2209089/76‑1 (MQ=255)
|
CAACCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCATGTC > REL606/3941513‑3941658
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
