breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
JC JC | 784,729 | Δ2 bp :: IS186 (+) +9 bp | coding (598‑606/2262 nt) | ybhJ → | predicted hydratase |
MC JC | 1,304,651 | Δ1,625 bp | [yciU]–cls | [yciU], cls | |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
JC | 2,103,918 | (CCAG)7→8 | coding (185/216 nt) | ECB_01992 → | hypothetical protein |
RA | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,488,791 | A→C | F139C (TTC→TGC) | glpR ← | DNA‑binding transcriptional repressor |
RA | 3,497,638 | A→C | F133C (TTC→TGC) | glgP ← | glycogen phosphorylase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 3,941,586 | G→A | V396M (GTG→ATG) | wecF → | putative enterobacterial common antigen polymerase |
RA | 4,108,375 | C→T | R43H (CGT→CAT) | metJ ← | transcriptional repressor protein MetJ |
RA | 4,163,127 | C→T | R758C (CGT→TGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 664687 | 0 (0.000) | 121 (0.790) | 71/144 | 0.9 | 98.8% | intergenic (‑78/‑48) | gltL/insJ‑2 | ATP‑binding protein of glutamate/aspartate transport system; b0652_1/IS150 hypothetical protein |
? | REL606 | 666134 = | 3 (0.020) | pseudogene (354/387 nt) | gltL | ATP‑binding protein of glutamate/aspartate transport system; b0652_1 |