breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 784,729 Δ2 bp :: IS186 (+) +9 bp coding (598‑606/2262 nt) ybhJ → predicted hydratase
MC JC 1,304,651 Δ1,625 bp [yciU]cls [yciU], cls
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
JC 2,103,918 (CCAG)7→8 coding (185/216 nt) ECB_01992 → hypothetical protein
RA 2,131,466 (A)9→8 intergenic (‑125/+175) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,488,791 A→C F139C (TTC→TGC)  glpR ← DNA‑binding transcriptional repressor
RA 3,497,638 A→C F133C (TTC→TGC)  glgP ← glycogen phosphorylase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,972 (T)7→8 intergenic (‑173/‑150) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 3,941,586 G→A V396M (GTG→ATG)  wecF → putative enterobacterial common antigen polymerase
RA 4,108,375 C→T R43H (CGT→CAT)  metJ ← transcriptional repressor protein MetJ
RA 4,163,127 C→T R758C (CGT→TGT)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 6646870 (0.000)121 (0.790) 71/144 0.9 98.8% intergenic (‑78/‑48) gltL/insJ‑2 ATP‑binding protein of glutamate/aspartate transport system; b0652_1/IS150 hypothetical protein
?REL606 666134 = 3 (0.020)pseudogene (354/387 nt) gltL ATP‑binding protein of glutamate/aspartate transport system; b0652_1