breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 223 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606720,4660GC61.5% ‑4.9 / 11.0 13A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6064330TG54.4% 52.3 / 100.5 68V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL606330,4500AC51.4% 44.5 / 55.1 35V266G (GTT→GGT) cynRDNA‑binding transcriptional dual regulator
*REL6061,854,4010AC49.2% 55.6 / 33.2 62K88N (AAA→AACyeaOhypothetical protein
*REL6062,515,9090AC49.1% 68.0 / 29.8 59V260G (GTG→GGG) ypfIpredicted hydrolase
*REL606895,8560GC45.0% 18.8 / 14.5 20A26P (GCC→CCC) ECB_00836putative Regulatory protein
*REL6063,472,7000CG44.1% 44.7 / 75.0 68A290G (GCG→GGG) yhgApredicted transposase
*REL606720,4640CG42.9% 5.0 / 12.8 14A22G (GCC→GGC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6063,774,9140CG42.0% 171.5 / 128.2 131A20P (GCC→CCC) yicJpredicted transporter
*REL6064,392,5560CT41.9% 96.6 / 73.4 105L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6064,554,8300AG41.5% 58.4 / 30.0 65intergenic (+30/+27)yjiV/mcrCb4486; conserved protein/5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
*REL6061,096,0320CG39.7% 93.2 / 57.8 68G148G (GGC→GGGputPproline:sodium symporter
*REL6061,047,3850CG39.3% 37.3 / 20.6 28S77R (AGC→AGGyccXpredicted acylphosphatase
*REL606691,8520TG38.2% 187.7 / 18.9 110intergenic (+17/‑33)ybfM/ybfNpredicted outer membrane porin/predicted lipoprotein
*REL6061,906,4300AC37.8% 219.8 / 38.9 111V329G (GTG→GGG) ptrBprotease II
*REL6061,149,6930TG37.0% 140.4 / 18.9 81G132G (GGT→GGGflgGflagellar component of cell‑distal portion of basal‑body rod
*REL6063,378,8850TG37.0% 290.3 / 30.2 138T129P (ACC→CCC) rplB50S ribosomal protein L2
*REL6062,376,3480TG36.5% 202.2 / 17.7 104T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL606341,6780TG35.2% 165.8 / 47.6 88V171G (GTG→GGG) mhpA3‑(3‑hydroxyphenyl)propionate hydroxylase
*REL6062,044,3570AC35.1% 301.1 / 69.1 148V284G (GTG→GGG) rfbDdTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2103888 =0 (0.000)47 (0.470) 29/96 3.6 95.0% coding (155/216 nt) ECB_01992 hypothetical protein
?REL606 = 2103891 5 (0.050)coding (158/216 nt) ECB_01992 hypothetical protein
* ? REL606 = 2037696103 (0.650)3 (0.020) 3/134 17.6 2.9% coding (435/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2037796 110 (0.780)coding (335/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 3737289 =165 (1.040)4 (0.030) 3/138 17.9 3.0% coding (538/1254 nt) waaL Lipid A‑core, surface polymer ligase
?REL606 3737758 = 106 (0.730)coding (69/1254 nt) waaL Lipid A‑core, surface polymer ligase
* ? REL606 = 2680757182 (1.150)3 (0.020) 3/138 17.9 2.0% coding (283/897 nt) ECB_02511 ParB family protein
?REL606 = 2681225 130 (0.890)coding (751/897 nt) ECB_02511 ParB family protein
* ? REL606 1219463 =149 (0.940)3 (0.020) 3/138 17.9 2.0% intergenic (+101/‑299) ymgF/ycgH hypothetical protein/predicted protein; b4491
?REL606 1219727 = 153 (1.050)intergenic (+365/‑35) ymgF/ycgH hypothetical protein/predicted protein; b4491
* ? REL606 3298204 =141 (0.890)4 (0.030) 3/146 18.4 2.5% coding (45/876 nt) nanK N‑acetylmannosamine kinase
?REL606 = 4266119 171 (1.110)coding (186/1959 nt) acs acetyl‑coenzyme A synthetase
* ? REL606 3410839 =128 (0.810)3 (0.020) 3/148 18.5 2.3% coding (1646/1914 nt) yheS fused predicted transporter subunits of ABC superfamily: ATP‑binding components
?REL606 3410860 = 130 (0.830)coding (1667/1914 nt) yheS fused predicted transporter subunits of ABC superfamily: ATP‑binding components