breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 223 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 720,466 | 0 | G | C | 61.5% | ‑4.9 / 11.0 | 13 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 433 | 0 | T | G | 54.4% | 52.3 / 100.5 | 68 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 330,450 | 0 | A | C | 51.4% | 44.5 / 55.1 | 35 | V266G (GTT→GGT) | cynR | DNA‑binding transcriptional dual regulator |
* | REL606 | 1,854,401 | 0 | A | C | 49.2% | 55.6 / 33.2 | 62 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,515,909 | 0 | A | C | 49.1% | 68.0 / 29.8 | 59 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 895,856 | 0 | G | C | 45.0% | 18.8 / 14.5 | 20 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
* | REL606 | 3,472,700 | 0 | C | G | 44.1% | 44.7 / 75.0 | 68 | A290G (GCG→GGG) | yhgA | predicted transposase |
* | REL606 | 720,464 | 0 | C | G | 42.9% | 5.0 / 12.8 | 14 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 3,774,914 | 0 | C | G | 42.0% | 171.5 / 128.2 | 131 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 4,392,556 | 0 | C | T | 41.9% | 96.6 / 73.4 | 105 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 4,554,830 | 0 | A | G | 41.5% | 58.4 / 30.0 | 65 | intergenic (+30/+27) | yjiV/mcrC | b4486; conserved protein/5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC |
* | REL606 | 1,096,032 | 0 | C | G | 39.7% | 93.2 / 57.8 | 68 | G148G (GGC→GGG) | putP | proline:sodium symporter |
* | REL606 | 1,047,385 | 0 | C | G | 39.3% | 37.3 / 20.6 | 28 | S77R (AGC→AGG) | yccX | predicted acylphosphatase |
* | REL606 | 691,852 | 0 | T | G | 38.2% | 187.7 / 18.9 | 110 | intergenic (+17/‑33) | ybfM/ybfN | predicted outer membrane porin/predicted lipoprotein |
* | REL606 | 1,906,430 | 0 | A | C | 37.8% | 219.8 / 38.9 | 111 | V329G (GTG→GGG) | ptrB | protease II |
* | REL606 | 1,149,693 | 0 | T | G | 37.0% | 140.4 / 18.9 | 81 | G132G (GGT→GGG) | flgG | flagellar component of cell‑distal portion of basal‑body rod |
* | REL606 | 3,378,885 | 0 | T | G | 37.0% | 290.3 / 30.2 | 138 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
* | REL606 | 2,376,348 | 0 | T | G | 36.5% | 202.2 / 17.7 | 104 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 341,678 | 0 | T | G | 35.2% | 165.8 / 47.6 | 88 | V171G (GTG→GGG) | mhpA | 3‑(3‑hydroxyphenyl)propionate hydroxylase |
* | REL606 | 2,044,357 | 0 | A | C | 35.1% | 301.1 / 69.1 | 148 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2103888 = | 0 (0.000) | 47 (0.470) | 29/96 | 3.6 | 95.0% | coding (155/216 nt) | ECB_01992 | hypothetical protein |
? | REL606 | = 2103891 | 5 (0.050) | coding (158/216 nt) | ECB_01992 | hypothetical protein | |||||
* | ? | REL606 | = 2037696 | 103 (0.650) | 3 (0.020) | 3/134 | 17.6 | 2.9% | coding (435/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2037796 | 110 (0.780) | coding (335/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 3737289 = | 165 (1.040) | 4 (0.030) | 3/138 | 17.9 | 3.0% | coding (538/1254 nt) | waaL | Lipid A‑core, surface polymer ligase |
? | REL606 | 3737758 = | 106 (0.730) | coding (69/1254 nt) | waaL | Lipid A‑core, surface polymer ligase | |||||
* | ? | REL606 | = 2680757 | 182 (1.150) | 3 (0.020) | 3/138 | 17.9 | 2.0% | coding (283/897 nt) | ECB_02511 | ParB family protein |
? | REL606 | = 2681225 | 130 (0.890) | coding (751/897 nt) | ECB_02511 | ParB family protein | |||||
* | ? | REL606 | 1219463 = | 149 (0.940) | 3 (0.020) | 3/138 | 17.9 | 2.0% | intergenic (+101/‑299) | ymgF/ycgH | hypothetical protein/predicted protein; b4491 |
? | REL606 | 1219727 = | 153 (1.050) | intergenic (+365/‑35) | ymgF/ycgH | hypothetical protein/predicted protein; b4491 | |||||
* | ? | REL606 | 3298204 = | 141 (0.890) | 4 (0.030) | 3/146 | 18.4 | 2.5% | coding (45/876 nt) | nanK | N‑acetylmannosamine kinase |
? | REL606 | = 4266119 | 171 (1.110) | coding (186/1959 nt) | acs | acetyl‑coenzyme A synthetase | |||||
* | ? | REL606 | 3410839 = | 128 (0.810) | 3 (0.020) | 3/148 | 18.5 | 2.3% | coding (1646/1914 nt) | yheS | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
? | REL606 | 3410860 = | 130 (0.830) | coding (1667/1914 nt) | yheS | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |