Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F1 I1 R1
|
14 |
40.5 |
2605368 |
96.4% |
2511574 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,705,603 |
G→A |
intergenic (+65/‑26) |
ygaH → / → emrR |
predicted inner membrane protein/DNA‑binding transcriptional repressor of microcin B17 synthesis and multidrug efflux |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,705,603 | 0 | G | A | 93.3%
| 151.8
/ 0.3
| 45 | intergenic (+65/‑26) | ygaH/emrR | predicted inner membrane protein/DNA‑binding transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (24/18); total (27/18) |
| Fisher's exact test for biased strand distribution p-value = 2.64e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGC > REL606/2705530‑2705673
|
aTTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACt > 1:1073107/1‑76 (MQ=255)
atataACCCACAAGAATCATTTTTCTACAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGAGTGaa > 1:1288698/1‑76 (MQ=255)
tataACCCCCAAGAATCATTTTTCTAAAACAATACATTGACTTTGTTTTTCACTGTCGTTACTAGATAGACTGcaa > 1:1178537/1‑76 (MQ=255)
aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTaa < 2:671261/76‑1 (MQ=255)
ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATg < 2:742708/76‑1 (MQ=255)
cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGa < 2:1018726/76‑1 (MQ=255)
acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAg < 2:214812/76‑1 (MQ=255)
aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTc < 1:487767/76‑1 (MQ=255)
gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCa < 2:248839/76‑1 (MQ=255)
gAATCATTTTTCTAAAACAATACATTTACTTTATTTGACACTGTCGTTACTATATCGACTGAAATTAATGAGGTCa > 2:1289763/1‑76 (MQ=255)
tCATATTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATAc > 1:80569/1‑76 (MQ=255)
aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATAccc > 1:291461/1‑76 (MQ=255)
tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCa < 2:1028764/76‑1 (MQ=255)
tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCa > 1:332591/1‑76 (MQ=255)
tttttCTAAAACAATACANTTACTTTATTTGTCACTGTCCTTACTAGATCGACTGAAATTAATGAGGTCATACCCa > 1:1241785/1‑76 (MQ=255)
ntttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAANGAGGTCATACCCaa > 2:184632/2‑76 (MQ=255)
tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCaaa > 1:181533/1‑76 (MQ=255)
cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATgg > 1:538776/1‑76 (MQ=255)
tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGa > 1:527500/1‑76 (MQ=255)
aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACTCAAATGGATa < 1:510235/76‑1 (MQ=255)
aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATa > 1:1003686/1‑76 (MQ=255)
aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATa < 1:510250/76‑1 (MQ=255)
aaCACTACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAg < 1:1284688/76‑1 (MQ=255)
aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAg < 1:537168/76‑1 (MQ=255)
tACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGt < 1:987729/76‑1 (MQ=255)
tttACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTAc > 1:928858/1‑76 (MQ=255)
ttACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACg > 1:831821/1‑76 (MQ=255)
aCTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGcc > 1:585683/1‑76 (MQ=255)
aCTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGcc > 1:281472/1‑76 (MQ=255)
cTTTATTTGTCACTGACGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGccc < 2:37816/76‑1 (MQ=255)
ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAt > 2:1054564/1‑76 (MQ=255)
ttATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAt > 1:1049734/1‑76 (MQ=255)
aTTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg > 2:799317/1‑76 (MQ=255)
tttGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGa > 1:478278/1‑76 (MQ=255)
tttGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGa < 2:1291646/76‑1 (MQ=255)
tGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc > 1:597619/1‑76 (MQ=255)
gTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACANAANTGGATAGTTCGTTTNCGCCCATTGAACa > 2:1174809/1‑76 (MQ=255)
tCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa > 1:725211/1‑76 (MQ=255)
cACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa < 2:245402/76‑1 (MQ=255)
cACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa < 2:245403/76‑1 (MQ=255)
tGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc > 1:997039/1‑76 (MQ=255)
gTCGNTACTATATCGACTGAAATGAATGAGGTCATACCCNAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt < 1:161244/76‑1 (MQ=255)
tCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa > 1:579163/1‑76 (MQ=255)
cGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaa > 1:1076496/1‑76 (MQ=255)
gTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa > 1:839979/1‑76 (MQ=255)
gTTACTATATCGACTGAAANTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa > 1:1216578/1‑76 (MQ=255)
tataTCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc < 2:648806/76‑1 (MQ=255)
tatCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc < 1:1015488/76‑1 (MQ=255)
|
ATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGC > REL606/2705530‑2705673
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
