Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F1 I1 R1
|
15 |
26.4 |
1686296 |
95.9% |
1617157 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,089 |
A→G |
E745G (GAA→GGA) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,089 | 0 | A | G | 94.4%
| 127.6
/ ‑4.0
| 36 | E745G (GAA→GGA) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (13/21); total (13/23) |
| Fisher's exact test for biased strand distribution p-value = 5.25e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGA > REL606/4163016‑4163157
|
ggtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAg < 2:800106/76‑1 (MQ=255)
ggtgTCGTTCAGTACGTGGATGCTTCCNATATCGTNNTCAAAGTTAACGAAGACGAGTTGTATCCTGGGGGAGCAg > 1:826456/1‑76 (MQ=255)
gtgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAgg < 1:574634/76‑1 (MQ=255)
gtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTa > 2:771056/1‑76 (MQ=255)
gtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTa > 1:561486/1‑76 (MQ=255)
cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATc < 2:360474/76‑1 (MQ=255)
cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATc > 2:191908/1‑76 (MQ=255)
tCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGAc > 2:340190/1‑76 (MQ=255)
aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCAGGGTGAAGCAGGTATCGACAt < 1:421597/76‑1 (MQ=255)
gTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATc < 1:442920/76‑1 (MQ=255)
tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCt > 2:24815/1‑76 (MQ=255)
aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTa < 1:373468/76‑1 (MQ=255)
aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTa > 2:503092/1‑76 (MQ=255)
tGGATGCTTCCCGTATCGTAATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACaa > 1:716172/1‑76 (MQ=255)
ggATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAAc < 1:199792/76‑1 (MQ=255)
ggATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAAc < 2:183314/76‑1 (MQ=255)
cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATACGGGTGGAGCAGGTATCGACATCTACAACCTGAc < 1:258134/76‑1 (MQ=255)
ttCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGAcc < 1:407537/76‑1 (MQ=255)
cccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAACACGTATCGACATCTACAACCTGACCac > 1:129630/1‑75 (MQ=255)
ccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCGGGGTGGAGCAGGTATCGACATCTACAACCTGACCaaa < 1:706586/76‑1 (MQ=255)
ccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCaaa > 2:391684/1‑76 (MQ=255)
gTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAAGTCTCGACATCTACAACCTGACCAAAtc > 1:126207/1‑75 (MQ=255)
tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATac < 1:159682/76‑1 (MQ=255)
tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATac < 1:492115/76‑1 (MQ=255)
tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGNATCGACATCTACAACCTGACCAAATac < 2:1426/76‑1 (MQ=255)
tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGANATCTACATCCNGACCAAATac < 2:830025/76‑1 (MQ=255)
gTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACAccc < 2:259029/76‑1 (MQ=255)
ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 2:289881/1‑76 (MQ=255)
tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGt > 1:375889/1‑76 (MQ=255)
aaaGTTAACGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTa < 2:561486/76‑1 (MQ=255)
cGAAGACGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAac > 2:440611/1‑76 (MQ=255)
gACGAGATGTATCCGGGTGGAGCAGGTATCGACATCAACTACCTGACCNAATACACCCGTTCTAACCAGAACACCt < 1:739415/76‑1 (MQ=255)
aCGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg > 1:661725/1‑76 (MQ=255)
cGAGATGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGt < 2:672774/76‑1 (MQ=255)
aTGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCa < 2:548509/76‑1 (MQ=255)
tGTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa < 2:661725/76‑1 (MQ=255)
gTATCCGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACTAGAACACCTGTATCAAc < 1:66766/76‑1 (MQ=255)
ccGGGTGGAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGa < 2:281577/76‑1 (MQ=255)
|
GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGA > REL606/4163016‑4163157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
