breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	1,004,259	(A)5→6	intergenic (‑185/+417)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
RA	1,071,973	C→T	A166T (GCT→ACT)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA	1,290,999	C→T	Q322* (CAA→TAA)	hnr →	response regulator of RpoS
RA	1,305,548	T→C	D231G (GAT→GGT)	cls ←	cardiolipin synthetase
RA	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I
JC	1,733,297	(CGTATGAA)1→2	coding (333/1413 nt)	pykF →	pyruvate kinase
RA	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein
MC JC	3,512,038	Δ415 bp		[yhhX]	[yhhX]
RA	3,762,741	A→T	K662I (AAA→ATA)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA	3,928,343	G→A	A43T (GCA→ACA)	rho →	transcription termination factor Rho
RA	4,163,089	A→G	E745G (GAA→GGA)	rpoB →	DNA‑directed RNA polymerase subunit beta
RA	4,201,969	T→G	L197F (TTA→TTC)	iclR ←	DNA‑binding transcriptional repressor
JC JC	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	589613–590322	593391	3070–3779	8 [5]	[5] 6	[insK‑1]–[fepA]	[insK‑1],[insB‑7],insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
*	*	÷	REL606	593571	619825–619117	25547–26255	6 [5]	[4] 6	[fepA]–[insA‑8]	[fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]