Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
17 |
44.3 |
2973330 |
95.1% |
2827636 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,901 |
G→T |
A229S (GCT→TCT) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,901 | 0 | G | T | 100.0%
| 136.0
/ NA
| 38 | A229S (GCT→TCT) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (23/15); total (23/15) |
GATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCC > REL606/3928828‑3928973
|
gATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCt < 1:665988/76‑1 (MQ=255)
gATGGTTCTGCTGATCAACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCt > 2:923982/1‑76 (MQ=255)
gTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTa < 2:1000253/76‑1 (MQ=255)
tCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTAcc > 1:994575/1‑76 (MQ=255)
ctgctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCt < 2:800661/76‑1 (MQ=255)
ctgctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCt > 1:333560/1‑76 (MQ=255)
tgctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCtt > 2:987333/1‑76 (MQ=255)
tgATCGACNAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGTGGAAGTTGGTTCTTCTAACTTTGa > 1:1428537/1‑76 (MQ=255)
tgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGCGAAGTTGTTTCTTCTACCTTTGa > 1:1096191/1‑76 (MQ=255)
gATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGAc < 1:885819/76‑1 (MQ=255)
gATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGAc < 2:321108/76‑1 (MQ=255)
aTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACg > 1:1307692/1‑76 (MQ=255)
cgaACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAAccc > 2:280379/1‑76 (MQ=255)
cgaACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAAccc > 1:331830/1‑76 (MQ=255)
aCGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCa > 2:1242742/1‑76 (MQ=255)
tCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCAtct > 1:434368/1‑76 (MQ=255)
cGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCg < 2:1100165/76‑1 (MQ=255)
cGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCg > 2:1138188/1‑76 (MQ=255)
gaagaaGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGcc > 2:790968/1‑76 (MQ=255)
aagaagTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCa < 1:46154/76‑1 (MQ=255)
agaagTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCAc < 1:555199/76‑1 (MQ=255)
aagTAACCGAGATGCAGNCTCTGGTAAAAGGGGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATATCGCCACGt > 1:190721/1‑76 (MQ=255)
aaCCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAg > 1:1079627/1‑76 (MQ=255)
cGAGATGCAGCGTCTGGTAAAAGGAGAANGTGTTTCTTCTACCTCTGAAGNACCCGCATCTCGCCACGTTCAGGtt > 1:211850/1‑76 (MQ=255)
cGAGANGGAGCGTCTGGTAAAAGGTGAACATGTTTCTTCTACCTTTGACGAACCCGCATCTNGCCACGTTCAGGtt > 2:1484169/1‑76 (MQ=255)
gagaTGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTg < 2:1311071/76‑1 (MQ=255)
aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCgg > 1:739403/1‑76 (MQ=255)
aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCgg < 1:1020783/76‑1 (MQ=255)
gTCTGGTAAAAGGTGAAGTTGTTTCTTNTANCTNTGGCGAANCCTCATGTCGCCACGTTCAGGTTGCGGAAATGGt < 1:1287634/76‑1 (MQ=255)
cTGGTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGa > 1:999459/1‑76 (MQ=255)
ggTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGATCCCGCATCTCGNCACGTTCAGGTTGCGGAAATGGTGATc < 1:1292244/76‑1 (MQ=255)
gTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCg > 1:1050945/1‑76 (MQ=255)
gTAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCg > 2:1316424/1‑76 (MQ=255)
tAAAAGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCga > 1:1412505/1‑76 (MQ=255)
tAAAAAGTGAAGTTGTTTCTTCTACCTTTGACGAGCCCGCATCTCGCCACGTTCAGGTTGCGGTAATGGTGATCga < 2:55911/76‑1 (MQ=255)
aataGGTGAAGTTGTTTCTTCTACCTTTGACGAGCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCgag > 1:1354361/4‑76 (MQ=255)
aaaaGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCgag < 1:642325/76‑1 (MQ=255)
aGGTGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAg > 1:665479/1‑76 (MQ=255)
tGAAGTTGTTTCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGcc < 2:1394855/76‑1 (MQ=255)
ttgtttCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACg > 1:703302/1‑76 (MQ=255)
ttgtttCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACg < 1:1378164/76‑1 (MQ=255)
gtttCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGcc < 2:177024/76‑1 (MQ=255)
|
GATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCC > REL606/3928828‑3928973
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
